Gardnerella vaginalis-associated bacterial vaginosis in Bulgarian women

Gergova, Raina; Mitov, Ivan

doi:10.1016/j.bjid.2012.10.026

Cited by 18 publications

(14 citation statements)

References 30 publications

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“…The high load of this synergistic bacterial combination causes a more severe infection and poses a significant risk of preterm birth (7,30). A combination of BV with TV coinfection was found in 17.93% of the examined patients, which was more than the incidence of 13.69% reported in a previous Bulgarian study (8). In most of these samples, all 3 pathogens (G. vaginalis, A. vaginae, and Mobiluncus spp.)…”

Section: Resultsmentioning

confidence: 75%

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Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women

Tosheva-Daskalova¹,

Mitov²,

Gergova³

2017

Turk J Med Sci

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Background/aim: This study aimed to investigate the correlation between the prevalence of problematic pathogens and the clinical status of women with bacterial vaginosis (BV).Materials and methods: Gardnerella vaginalis, Atopobium vaginae, and Mobiluncus spp. were detected using a multiplex PCR assay, and their role in the infection of Bulgarian women with clinically heterogeneous BV was evaluated. Results:The predominant BV-associated pathogen identified was G. vaginalis with an incidence of 98.39%, followed by A. vaginae (68.05%) and Mobiluncus spp. at 17.01%. The coexistence of A. vaginae and G. vaginalis was more common in women with discharge (in 72.04%) and in patients with chronic recurrent BV than among asymptomatic or newly diagnosed BV cases (P < 0.05). Mobiluncus spp. was detected mostly in coinfections, in association with Trichomonas vaginalis. The coinfections were predominantly related to recurrent BV and with complications (P < 0.05). Conclusion:This is the first study about the correlation between problematic pathogens and clinically heterogeneous BV in Bulgarian women. High frequency of infection with key BV-related pathogens was observed in childbearing women. The incidence was shown to often correlate with coexistent T. vaginalis, with severity of infection, and with complicated and recurrent BV after unsuccessful treatments. Screening should be considered in reproductive health programs.

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Section: Resultsmentioning

confidence: 75%

“…Bacteria such as G. vaginalis, Atopobium vaginae, and Mobiluncus spp. are recognized as predominant vaginal pathogens and as sensitive indicators in the diagnosis of BV, although many other BV-related pathogens have been found with variable frequency (7)(8)(9)(10)(11). A. vaginae and Mobiluncus spp.…”

Section: Introductionmentioning

confidence: 99%

Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women

Tosheva-Daskalova¹,

Mitov²,

Gergova³

2017

Turk J Med Sci

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“…Microorganisms mostly detected in BV were Gardnerella vaginalis and Atopobium vaginae, with prevalence in BV ranging between 47.8-99% (Gardnerella vaginalis) and 75-95% (Atopobium vaginae) without significant difference in prevalence between pregnant and non-pregnant women [4,8,9]. In addition, the coexistence of these two microbes was documented in 78-96% of samples with BV [10].…”

Section: Introductionmentioning

confidence: 90%

“…Proper diagnosis of BV is demanding in terms of sensitivity and specificity for precise outlining of the group of patents in need of treatment. The majority of studies have agreed on the fact that is not possible to cultivate microaerophilic or anaerobic residents of the vagina with complete efficiency [3][4][5][6]. With the introduction of molecular detection -polymerase chain reaction (PCR) -of the aforementioned bacteria, this problem has been surpassed.…”

Section: Introductionmentioning

confidence: 99%

Molecular diagnosis of bacterial vaginosis: Prevalence of Gardnerella vaginalis and Atopobium vaginae in pregnant women

et al. 2018

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Introduction/Objective Bacterial vaginosis (BV) is defined as disequilibrium of vaginal microbiota due to proliferation of Gram-negative/variable anaerobes and reduction/depletion of vaginal lactobacilli. Difficulties in interpreting microscopically categorized findings in diagnosis of BV need a molecular analysis of bacteria present in vaginal discharge of patients. In this regard, we performed real-time qPCR analysis of vaginal discharge samples with the goal to explore in which extent prevalence and amount of anaerobes, Gardnerella vaginalis and Atopobium vaginae, are related to findings obtained by microscopy. Methods This study enrolled 111 asymptomatic pregnant women between 24 and 28 weeks of pregnancy. Gram-stained vaginal smears were evaluated microscopically. Afterwards, DNA of bacteria was extracted from Gram slides and real-time qPCR was performed with the aim to detect and quantify G. vaginalis and A. vaginae. Results The data of our study showed that 53.2% of patients had normal results, while 20.7% and 26.1% of patients had intermediary (IMD) and BV results, respectively. G. vaginalis and A. vaginae were more frequently found in IMD and BV than in healthy patients; also, the average bacterial number of G. vaginalis and A. vaginae were significantly higher in BV and IMD than in the group with normal findings (p = 0.000). Comparing mutual relation of G. vaginalis and A. vaginae, the prevalence and number of G. vaginalis were in all groups significantly higher than A. vaginae. Conclusion The data of our study have shown that in distinguishing normal from BV findings, quantification of bacteria may be more important than just molecular detection of bacteria.

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“…Of these infections BV is the most common infection found in vaginal tract which causes vaginal discharge and thus leading to vaginal disorder in women of reproductive age. 5,6 The present study was intended to perform the comparative evaluation of Nugent and Amsel criteria in diagnosis of various types of female genital tract infections in a tertiary care hospital, Karnataka.…”

Section: Sexually Transmitted Disease (Std) Organisms Whichmentioning

confidence: 99%

Analysis of Nugent and Amsel Criteria for the Detection of Female Genital Tract Infections in a Tertiary Care Hospital, Coastal Karnataka

Kumari¹,

Antony²

2018

jemds

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BACKGROUND Female genital tract infections are polymicrobial in nature. Among the genital infections, bacterial vaginosis is the most common infection caused by anaerobic and microaerophilic organisms such as Bacteroides fragilis group, Mobiluncus species, Porphyromonas species, Prevotella group and Gardnerella vaginalis. Objective-Comparative evaluation of Nugent and Amsel criteria in diagnosis of various types of female genital tract infections. MATERIALS AND METHODS The present descriptive study was conducted in a tertiary care hospital in coastal Karnataka, South India during the period from July 2013 to June 2016. Of the 1217 women under study, 1054 cases were taken with complaints of vaginal discharge and 163 were included as control, which belongs to normal vaginal flora, confirmed by microscopy in the Dept. of Microbiology, Father Muller Medical College, Mangalore. High vaginal swabs from women of reproductive age group (15-45 years) and 163 age matched control group were examined by preliminary examination. RESULTS By employing Amsel and Nugent criteria, out of 1054 vaginal samples 415 (39.4%) were Normal vaginal flora, Vaginal Lactobacillosis 117 (9.3%), Bacterial vaginosis 315 (29.9%), Intermediate Bacterial vaginosis 11 (1.0%), Vulvovaginitis 6 (0.6%), Cervicitis 4 (0.4%), HIV 2 (0.2%), Candidiasis 106 (10.1%), Group B Streptococcus with Antenatal cases 73 (6.9%) and Trichomoniasis 5 (0.5%). No vaginal infections were detected from the control group. CONCLUSION BV, Candidiasis, Trichomoniasis and Vulvovaginitis are the most common conditions present among female genital tract infections. Nugent and Amsel criteria are the most cost effective and less time-consuming tests for the diagnosis of vaginal infections.

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Gardnerella vaginalis-associated bacterial vaginosis in Bulgarian women

Abstract: This is the first comparative investigation on the epidemiology of G. vaginalis-associated BV in Bulgaria. The established highest frequency in the young Bulgarian women (21-30 years) is alarming and should be considered in prophylaxis and reproductive programmes.

Cited by 18 publications

References 30 publications

Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women

Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women

Molecular diagnosis of bacterial vaginosis: Prevalence of Gardnerella vaginalis and Atopobium vaginae in pregnant women

Analysis of Nugent and Amsel Criteria for the Detection of Female Genital Tract Infections in a Tertiary Care Hospital, Coastal Karnataka

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