Determination of urine tumor necrosis factor, IL-6, IL-8, and serum IL-6 in patients with hemorrhagic fever with renal syndrome

Fan, Wanhu; Liu, Xiaojing; Yue, Jinsheng

doi:10.1016/j.bjid.2012.10.002

Cited by 13 publications

(10 citation statements)

References 6 publications

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“…Finally, a multivariate stepwise forward and reverse logistic regression model was constructed, confirming the relevance of the IL-6 levels at the time of patient hospitalization in the prediction of the outcome of HCPS ( Table 5 ). It is well documented that IL-6 levels are significantly elevated in the serum of patients with HCPS, epidemic nephropathy (EP), and HFRS [ 5 , 12 , 35 ]. Consistent with our findings, other reports show that IL-6 levels are even higher in severe-disease patients compared with mild-disease patients [ 13 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Serum levels of interleukin-6 are linked to the severity of the disease caused by Andes Virus

et al. 2017

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Andes virus (ANDV) is the etiological agent of hantavirus cardiopulmonary syndrome in Chile. In this study, we evaluated the profile of the pro-inflammatory cytokines IL-1β, IL-12p70, IL-21, TNF-α, IFN-γ, IL-10 and IL-6 in serum samples of ANDV-infected patients at the time of hospitalization. The mean levels of circulating cytokines were determined by a Bead-Based Multiplex assay coupled with Luminex detection technology, in order to compare 43 serum samples of healthy controls and 43 samples of ANDV-infected patients that had been categorized according to the severity of disease. When compared to the controls, no significant differences in IL-1β concentration were observed in ANDV-infected patients (p = 0.9672), whereas levels of IL-12p70 and IL-21 were significantly lower in infected cases (p = <0.0001). Significantly elevated levels of TNF-α, IFN-γ, IL-10, and IL-6 were detected in ANDV-infected individuals (p = <0.0001, 0.0036, <0.0001, <0.0001, respectively). Notably, IL-6 levels were significantly higher (40-fold) in the 22 patients with severe symptoms compared to the 21 individuals with mild symptoms (p = <0.0001). Using multivariate regression models, we show that IL-6 levels has a crude OR of 14.4 (CI: 3.3–63.1). In conclusion, the serum level of IL-6 is a significant predictor of the severity of the clinical outcome of ANDV-induced disease.

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Section: Discussionmentioning

confidence: 99%

Serum levels of interleukin-6 are linked to the severity of the disease caused by Andes Virus

et al. 2017

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“…HFRS has the characteristics of sudden onset and fast progression. Laboratory tests can only asses for indicators of platelet decrease, proteinuria, lymphocyte increase, and a CD4:CD8 ratio decrease . Many previous studies have demonstrated that high concentrations of IFN‐γ, IL‐10, CCL2, IL‐12p70, and sIL‐2R can be found in patient serum .…”

Section: Discussionmentioning

confidence: 99%

Cytokine response to Hantaan virus infection in patients with hemorrhagic fever with renal syndrome

Guo

Wang

et al. 2017

Journal of Medical Virology

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Hantaan virus (HTNV) infection of the human body causes a severe acute infectious disease known as hemorrhagic fever renal syndrome (HFRS). The aim of this study was to correlate patient cytokine profiles to HFRS severity. In this study, we discuss the clinical significance of evaluating HFRS treatment outcomes using cytokine information. The levels of 18 cytokines were quantitatively determined in three groups: 34 HTNV IgM+ cases, 63 HTNV IgM- negative cases, and 78 healthy volunteers. The level of 14 serum cytokines were higher in the patient group than that in the healthy control group. In the 34 HTNV IgM+ patient sera, a set of 27 cytokines was further assessed. The cytokines of TNF-β, IL-1ra, and IL-6 were detected at higher level in the IgM+ group than that in the IgM- group. The deterioration of HFRS was accompanied with multiple cytokines increased, such as IL-1ra, IL-12p70, IL-10, IP-10, IL-17, IL-2, and IL-6. Our data indicate that serum cytokine levels are associated with the progression of HFRS.

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“…Several previous studies reported an association of pro-inflammatory cytokines and severity of hantavirus infections [ 14 , 23 - 26 ]. There are only few reports about the role of anti-inflammatory and suppressive cytokines such as TGF-β in hantavirus infection.…”

Section: Discussionmentioning

confidence: 99%

Association of low serum TGF-β level in hantavirus infected patients with severe disease

et al. 2015

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BackgroundHantaviruses are emerging zoonotic pathogens which cause hemorrhagic fever with renal syndrome, an immune-mediated pathogenesis is discussed. The aim of the present study was to investigate the role of TGF-β expression in acute hantavirus infection.ResultsWe retrospectively studied 77 patients hospitalised with acute Puumala infection during a hantavirus epidemic in Germany in 2012. Hantavirus infection was confirmed by positive anti-Puumala hantavirus IgG and IgM. Plasma levels of transforming growth factor (TGF)-β1 and TGF-β2 were analysed. Based on glomerular filtration rate on admission, patients were divided in mild and severe course of disease. Puumala virus RNA was detected by PCR amplification of the viral L segment gene. Out of 77 Puumala virus infected patients, 52 (68%) were male. A seasonal distribution was detected in our cohort with a peak in summer 2012, the highest incidence was observed in the age group of 30–39 years. Puumala virus RNA was detectable in 4/77 cases. Patients with severe disease had a significant longer hospital stay than patients with mild disease (6.2 vs 3.6 days). Thrombocyte count (186 vs 225 per nl), serum TGF-β1 (74 vs 118 ng/l) and TGF-β2 (479 vs 586 pg/l) were significantly lower in severe compared to mild disease. However, C-reactive protein (CRP) was significantly higher in patients with severe disease (62 vs 40 mg/l). TGF-β1/Cr was the most sensitive and specific marker associated with renal dysfunction.ConclusionHigh serum CRP and low serum TGF-β in the early phase of hantavirus infection is associated with a severe course of disease. Our results support the hypothesis of an immune-mediated pathogenesis in hantavirus infection.

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Determination of urine tumor necrosis factor, IL-6, IL-8, and serum IL-6 in patients with hemorrhagic fever with renal syndrome

Cited by 13 publications

References 6 publications

Serum levels of interleukin-6 are linked to the severity of the disease caused by Andes Virus

Serum levels of interleukin-6 are linked to the severity of the disease caused by Andes Virus

Cytokine response to Hantaan virus infection in patients with hemorrhagic fever with renal syndrome

Association of low serum TGF-β level in hantavirus infected patients with severe disease

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