Morphine promotes migration and lung metastasis of mouse melanoma cells

Vaseghi, Golnaz; Dana, Nasim; Gharehbaghian, Ahmad; Abediny, Reza; Laher, Ismail; Javanmard, Shaghayegh Haghjooy

doi:10.1016/j.bjane.2021.10.019

Cited by 5 publications

(3 citation statements)

References 42 publications

(47 reference statements)

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“…The Klf1 (K74R) mutation also protected the mice from metastasis of melanoma cells and reduced melanoma growth in the subcutaneous cancer cell inoculation assay ( Shyu et al, 2022 ). Here, we used the pulmonary melanoma foci assay, which has been commonly used for quick analysis of early metastasis ( Vaseghi et al, 2023 ), to further characterize the higher cancer resistance of the Klf1 (K74R) mice with respect to the effects of gender/age/genetic background of the mice and the requirement for the homozygosity of the K74R mutation. The cultured melanoma B16-F10 cells used in this assay are poorly immunogenic and highly aggressive, thus being useful for monitoring early metastasis in various immunotherapy studies ( Vaseghi et al, 2023 ; Díaz-García et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Long-term hematopoietic transfer of the anti-cancer and lifespan-extending capabilities of a genetically engineered blood system by transplantation of bone marrow mononuclear cells

Wang

Hung

Liou

et al. 2024

eLife

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A causal relationship exists among the aging process, organ decay and dis-function, and the occurrence of various diseases including cancer. A genetically engineered mouse model, termed Eklf K74R/K74R or Eklf(K74R), carrying mutation on the well-conserved sumoylation site of the hematopoietic transcription factor KLF1/ EKLF has been generated that possesses extended lifespan and healthy characteristics including cancer resistance. We show that the high anti-cancer capability of the Eklf(K74R) mice are gender-, age-and genetic background-independent. Significantly, the anti-cancer capability and extended lifespan characteristics of Eklf(K74R) mice could be transferred to wild-type mice via transplantation of their bone marrow mononuclear cells. Targeted/global gene expression profiling analysis has identified changes of the expression of specific proteins and cellular pathways in the leukocytes of the Eklf(K74R) that are in the directions of anti-cancer and/or anti-aging. This study demonstrates the feasibility of developing a novel hematopoietic/ blood system for long-term anti-cancer and, potentially, for anti-aging.

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Section: Resultsmentioning

confidence: 99%

Long-term hematopoietic transfer of the anti-cancer and lifespan-extending capabilities of a genetically engineered blood system by transplantation of bone marrow mononuclear cells

Wang

Hung

Liou

et al. 2024

eLife

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show abstract

“…The Eklf (K74R) mutation also protected the mice from metastasis of melanoma cells and it reduced melanoma growth in the subcutaneous cancer cell inoculation assay 21 . Here we used the pulmonary melanoma foci assay, which has been commonly used for quick analysis of early metastasis 22 , to further characterize the higher cancer resistance of the Eklf (K74R) mice with respect to the effects of gender/age/genetic background of the mice and the requirement for the homozygosity of the K74R mutation. The cultured melanoma B16-F10 cells used in this assay are poorly immunogenic and highly aggressive thus being useful for monitoring early metastasis in various immunotherapy studies 22,23 .…”

Section: Resultsmentioning

confidence: 99%

Long-term Hematopoietic Transfer of the Anti-Cancer and Lifespan-Extending Capabilities of A Genetically Engineered Blood System by Transplantation of Bone Marrow Mononuclear Cells

Wang

Hung

Liou

et al. 2023

Preprint

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A causal relationship exists among the aging process, organ decay and dis-function, and the occurrence of various diseases including cancer. A genetically engineered mouse model, termedEklfK74R/K74RorEklf(K74R), carrying mutation on the well-conserved sumoylation site of the hematopoietic transcription factor KLF1/ EKLF has been generated that possesses extended lifespan and healthy characteristics including cancer resistance. We show that the high anti-cancer capability of theEklf(K74R) mice are gender-, age- and genetic background-independent. Significantly, the anti-cancer capability and extended lifespan characteristics ofEklf(K74R) mice could be transferred to wild-type mice via transplantation of their bone marrow mononuclear cells. Targeted/global gene expression profiling analysis has identified changes of the expression of specific proteins and cellular pathways in the leukocytes of theEklf(K74R) that are in the directions of anti-cancer and/or anti-aging. This study demonstrates the feasibility of developing a novel hematopoietic/ blood system for long-term anti-cancer and, potentially, for anti-aging.

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“…Several studies have investigated the effects of morphine on cancer patients, although the exact role of this agent in the regulation of tumor cell growth remains uncertain, which is also true in the case of signaling pathways. [ 15 24 ] Several reports have indicated the pro-apoptotic effects of opioids on cancer cells in vitro . [ 25 26 ] The anti-apoptotic effects of morphine on tumor cells have also been reported.…”

Section: Discussionmentioning

confidence: 99%

Effects of the co-administration of morphine and lipopolysaccharide on toll-like receptor-4/nuclear factor kappa β signaling pathway of MDA-MB-231 breast cancer cells

et al. 2023

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Background: The Toll-like receptor 4 (TLR4) gene promotes migration in adenocarcinoma cells. Morphine is an agonist for TLR4 that has a dual role in cancer development. The promoter or inhibitor role of morphine in cancer progression remains controversial. This study aims to evaluate the effects of morphine on the TLR4, myeloid differentiation primary response protein 88-dependent (MyD88), and nuclear factor-kappa B (NF-κB) expressions in the human MDA-MB-231 breast cancer cell line. Materials and Methods: The cells were examined after 24 hours of incubation with morphine using the Boyden chamber system. TLR4, MyD88, and NF-κB mRNA expressions were assessed using quantitative real-time polymerase chain reaction (RT-PCR). The concentration of interleukin-2 beta was also measured using the ELISA assay. Results: According to the findings, three doses of morphine (0.25, 1.25, and 0.025 μM) increased the expression of the TLR4 and NF-κB genes, whereas no significant change was observed in the mRNA expression of MyD88. Furthermore, treatment with morphine and lipopolysaccharide (LPS) significantly decreased the expression of TLR4, MyD88, and NF-κB. However, no significant change was observed in interleukin 2 beta concentration. Conclusions: These findings confirmed the excitatory effects of morphine on TRL4 expression and the MYD88 signaling pathway in vitro .

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Morphine promotes migration and lung metastasis of mouse melanoma cells

Cited by 5 publications

References 42 publications

Long-term hematopoietic transfer of the anti-cancer and lifespan-extending capabilities of a genetically engineered blood system by transplantation of bone marrow mononuclear cells

Long-term hematopoietic transfer of the anti-cancer and lifespan-extending capabilities of a genetically engineered blood system by transplantation of bone marrow mononuclear cells

Long-term Hematopoietic Transfer of the Anti-Cancer and Lifespan-Extending Capabilities of A Genetically Engineered Blood System by Transplantation of Bone Marrow Mononuclear Cells

Effects of the co-administration of morphine and lipopolysaccharide on toll-like receptor-4/nuclear factor kappa β signaling pathway of MDA-MB-231 breast cancer cells

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