Purpose Optimizing patient position and needle puncture site are important factors for successful neuraxial anesthesia. Two paramedian approaches are commonly utilized and we sought to determine whether variations of the seated position would increase the chance of puncture success. Methods We simulated paramedian needle passes on three-dimensional lumbar spine models registered to volumetric ultrasound data acquired from ten healthy volunteers in three different positions: 1) prone; 2) seated with thoracic and lumbar flexion; and 3) seated as in position 2, but with a 10°dorsal tilt. Simulated paramedian needle passes from the right side performed on validated models were used to determine L2-3 and L3-4 neuraxial target size and success. We selected two paramedian puncture sites according to standard anesthesia textbook descriptions: 10 mm lateral and 10 mm caudal from inferior edge of the superior spinous process as described