Comparative immunohistochemical assessment of the effect of repetitive anesthesia with isoflurane and sevoflurane on rat liver

Ruxanda, Flavia; Gal, Adrian Florin; Rațiu, Cristian; Mitre, Viorel; Rus, Vasile; Oană, Liviu

doi:10.1016/j.bjane.2015.02.003

Cited by 6 publications

(6 citation statements)

References 23 publications

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“…1A–D ), while only high concentrations of sevoflurane lead to significant damage to normal liver cells ( Supplementary Fig. 1E, F ), which is in accordance with previous studies 20 , 21 . These results suggest that sevoflurane may be more toxic to tumor cells with high proliferative capacity due to some mechanism, which is not fully understood at present.…”

Section: Resultssupporting

confidence: 92%

“…Translational findings based on a clinical trial revealed that sevoflurane protects hepatocytes from ischemic injury by reducing reactive oxygen species–associated signaling in hematopoietic stem cells 27 . Also, 2% sevoflurane does not trigger hepatocyte apoptosis in rat; it has no significant hepatotoxicity 20 . Even after 3 days of 3% sevoflurane exposure, there was no evidence of hepatic or renal toxicity both from histologic studies and from serum biomarkers 21 .…”

Section: Discussionmentioning

confidence: 94%

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Sevoflurane Inhibits Metastasis in Hepatocellular Carcinoma by Inhibiting MiR-665-Induced Activation of the ERK/MMP Pathway

Zhu

Peng

et al. 2022

Cell Transplant

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Recent evidence has indicated that inhalational anesthetics may affect the growth and malignant potential of tumor cells and ultimately influence tumor recurrence after surgery. Sevoflurane, a volatile anesthetic, is used extensively in hepatectomy. However, the effect of sevoflurane on the growth of hepatocellular carcinoma (HCC) cells remains unknown. The aim of this study was to explore the effects of sevoflurane on HCC metastasis and its potential mechanisms in the human HCC cell lines, HepG2 and SMMC7721. HepG2 and SMMC7721 cells were treated with 1.7%, 3.4%, and 5.1 % sevoflurane for 6 h. Cell migration was analyzed using invasion, migration, and scratch assays. Based on previous literature, several microRNAs (miRNAs) were screened to determine regulatory miRNA targets of sevoflurane in HepG2 and SMMC7721 cells; miR-665 was detected as a potential target and overexpressed or inhibited in HepG2 and SMMC7721 cells by a lentiviral system. The p-ERK/MMP pathway was also measured by western blotting. Sevoflurane inhibited the migration and invasion of HCC cells in a dose-dependent manner. It also inhibited miR-665 expression in HCC cells. We further observed that sevoflurane inhibited HCC metastasis via miR-665. Sevoﬂurane-induced downregulation of miRNA-665 led to phosphorylation of ERK and matrix metalloproteinase (MMP-9) via suppression of SPRED1. These results demonstrated that sevoflurane may inhibit invasion and migration via the p-ERK/MMP-9 signaling pathway in HCC cells.

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Section: Resultssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 94%

Sevoflurane Inhibits Metastasis in Hepatocellular Carcinoma by Inhibiting MiR-665-Induced Activation of the ERK/MMP Pathway

Zhu

Peng

et al. 2022

Cell Transplant

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“…The mechanism underlying isoflurane anesthesia may be associated with the action of the human glycine receptor ( 32 ). In recent years, additional functions of isoflurane have been reported in various types of disease ( 33 – 35 ). In the present study, the additional functions of isoflurane as an anesthetic for mice with myocardial ischemia during heart bypass surgery were analyzed.…”

Section: Discussionmentioning

confidence: 99%

Isoflurane reduces pain and inhibits apoptosis of myocardial cells through the phosphoinositide 3-kinase/protein kinaseï¿½B signaling pathway in mice during cardiac surgery

Lin

Yang

2018

Mol Med Report

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Heart bypass surgery is the most common treatment for myocardial ischemia. Clinical investigations have revealed that isoflurane anesthesia is efficient to alleviate pain during cardiac surgery, including heart bypass surgery. Previous studies have revealed the protective effects of isoflurane on myocardial cells of patients with myocardial ischemia during the perioperative period. The present study aimed to investigate the mechanism underlying the protective effects of isoflurane on myocardial cells in mice with myocardial ischemia. ELISA, flow cytometry, immunofluorescence and western blotting were used to analyze the effects of isoflurane anesthesia on myocardial cells. Briefly, myocardial cell apoptosis and viability, pain, phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) signaling pathway expression and the pharmacodynamics of isoflurane were studied in mice treated with isoflurane for heart bypass surgery. The results demonstrated that isoflurane anesthesia efficiently attenuated pain in mice during surgery. Viability and apoptosis of myocardial cells was also improved by isoflurane in vitro and in vivo. The PI3K/AKT pathway was upregulated in myocardial cells on day 3 post-operation. Mechanistically, isoflurane promoted PI3K/AKT activation, upregulated B-cell lymphoma 2 (Bcl-2)-associated X protein and Bcl-2 expression levels, and reduced the expression levels of caspase-3 and caspase-8 in myocardial cells. In conclusion, the findings indicated that isoflurane is beneficial for pain attenuation and inhibits apoptosis of myocardial cells via the PI3K/AKT signaling pathway in mice during cardiac surgery.

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“…In a previous study, the effect of repeated isoflurane and sevoflurane anesthesia on rat hepatocytes was investigated. The results suggested that the anesthetics do not present considerable hepatotoxicity [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

A case report of multiple anesthesia for pediatric surgery: 80 anesthesia applications in a period of 6 years

Oba

Türk

2018

BMC Anesthesiol

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BackgroundThe side and adverse effects of anesthesia and its neurotoxicity to children have become major concerns of anesthesiologists in recent years.Currently, no clinical trials have provided clear evidence indicating the suitable minimum age for a patient’s first anesthetic application, importance of anesthesia duration, number of anesthetic applications or interval between two consecutive anesthesia applications.A very rare case concerning the side, adverse and neurotoxic effects of multiple anesthesia in a child is presented.Case presentationA case of a 9-year-old child who received 80 applications of anesthesia in 6 years because of corrosive esophagitis is presented. The commonly used anesthetic agents were propofol, fentanyl, rocuronium and sevoflurane.ConclusionIn our case, there were no permanent side or adverse effects due to multiple anesthesia. The minimal psychological and scholastic problems of our case were tied to frequent hospitalization by the pediatric psychiatry consultation.

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Comparative immunohistochemical assessment of the effect of repetitive anesthesia with isoflurane and sevoflurane on rat liver

Abstract: The results suggest that the anesthetics do not present a considerable hepatotoxicity. The comparative assessment of the two anesthetics shows that sevoflurane is superior to isoflurane.

Cited by 6 publications

References 23 publications

Sevoflurane Inhibits Metastasis in Hepatocellular Carcinoma by Inhibiting MiR-665-Induced Activation of the ERK/MMP Pathway

Sevoflurane Inhibits Metastasis in Hepatocellular Carcinoma by Inhibiting MiR-665-Induced Activation of the ERK/MMP Pathway

Isoflurane reduces pain and inhibits apoptosis of myocardial cells through the phosphoinositide 3-kinase/protein kinaseï¿½B signaling pathway in mice during cardiac surgery

A case report of multiple anesthesia for pediatric surgery: 80 anesthesia applications in a period of 6 years

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