Efficacy of enzymatic mouth rinses for immobilisation of protective enzymes in the in situ pellicle

Hannig, Christian; Spies, Benedikt Christopher; Spitzmüller, Bettina; Hannig, Matthias

doi:10.1016/j.archoralbio.2009.10.004

Cited by 18 publications

(13 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, high intra-and interindividual variability of enzyme activities was shown. All these observations correspond to previous studies in adults and seem to be characteristic for initial bioadhesion processes in the oral cavity (3,4,11,27,32,33). Unexpectedly, despite clear definition of cariesfree and caries-active children, neither caries activity nor the specific oral milieu in children had any impact on enzyme activities in the acquired pellicle.…”

Section: Discussionsupporting

confidence: 89%

“…Glucosyltransferase activity was detected in the 30‐min pellicle of all children. The enzyme activities obtained in the children's in‐situ pellicle were in the same range as formerly recorded in adults . In general, high intra‐ and interindividual variability of enzyme activities was shown.…”

Section: Discussionsupporting

confidence: 77%

“…adults (4,12,18,27). In general, high intra-and interindividual variability of enzyme activities was shown.…”

Section: Discussionmentioning

confidence: 95%

See 2 more Smart Citations

Enzymes in the in‐situ pellicle of children with different caries activity

Grychtol

Viergutz

Pötschke

et al. 2015

European J Oral Sciences

View full text Add to dashboard Cite

The present study investigated, for the first time, enzymes in the in-situ pellicle of children. Furthermore, it was purposed to detect glucosyltransferase (GTF) isoforms in each child's pellicle. Twenty-four children (5-9 yr of age) participated in the study. Twelve were caries free with no decayed, missing, and filled teeth (dmft), whereas 12 had active caries (dmft ≥ 2, indicating at least two untreated carious lesions). Bovine enamel slabs, fixed on individual upper jaw splints, were utilized for pellicle formation in situ. After 3 and 30 min, samples were tested for amylase, lysozyme, and peroxidase activities; total GTF activity was examined only in the 30-min pellicle. Gold-immunolabelling was used to quantify the GTF B, C, and D isoforms in the pellicle by transmission electron microscopy (TEM). All enzymes tested were detected in the children's in-situ pellicle in an active conformation, and there were no significant differences in their levels of activity between caries-free and caries-active children. All GTF isoforms were found to be randomly distributed within all pellicle layers, althoug GTF B was only detected very sporadically. A significantly higher amount of GTF D was detected in the pellicle of caries-active children. Pellicle formation in children is characterized by uniformity and selectivity. Glucosyltransferase D might represent a possible biomarker for high caries risk in children.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 77%

See 1 more Smart Citation

Enzymes in the in‐situ pellicle of children with different caries activity

Grychtol

Viergutz

Pötschke

et al. 2015

European J Oral Sciences

View full text Add to dashboard Cite

show abstract

“…This group of proteins along with those involved in tissue regeneration account for around 52% of the identified pellicle proteins in total, and has the potential to be protective against oral infective pathologies like periodontal disease. Interestingly, there were proteins found, like myeloperoxidase, which maintain their defensive properties upon adsorption to the hydroxyapatite of enamel [23]. Along with myeloperoxidase, albumin and other identified pellicle proteins are known to have fluctuating concentrations in the gingival crevicular fluid, an exudate present between the periodontal tissue and the tooth, and in the AEP, depending on the degree of inflammatory response as a result of periodontal disease [24].…”

Section: Resultsmentioning

confidence: 99%

Proteome and Peptidome of Human Acquired Enamel Pellicle on Deciduous Teeth

Zimmerman

Custódio

Hatibović-Kofman

et al. 2013

IJMS

View full text Add to dashboard Cite

Understanding the composition and structure of the acquired enamel pellicle (AEP) has been a major goal in oral biology. Our lab has conducted studies on the composition of AEP formed on permanent enamel. The exhaustive exploration has provided a comprehensive identification of more than 100 proteins from AEP formed on permanent enamel. The AEP formed on deciduous enamel has not been subjected to the same biochemical characterization scrutiny as that of permanent enamel, despite the fact that deciduous enamel is structurally different from permanent enamel. We hypothesized that the AEP proteome and peptidome formed on deciduous enamel may also be composed of unique proteins, some of which may not be common with AEP of permanent enamel explored previously. Pellicle material was collected from 10 children (aged 18–54 months) and subjected to mass spectrometry analysis. A total of 76 pellicle proteins were identified from the deciduous pellicle proteome. In addition, 38 natural occurring AEP peptides were identified from 10 proteins, suggesting that primary AEP proteome/peptidome presents a unique proteome composition. This is the first study to provide a comprehensive investigation of in vivo AEP formed on deciduous enamel.

show abstract

“…Earlier research on these products showed that it was questionable whether these proteins can be immobilized in the acquired pellicle [Hannig et al, 2005]. However, recent studies on the efficacy of enzymatic toothpastes and mouthrinses showed that the immobilization of enzymes in an in situ pellicle can, indeed, be achieved by using toothpaste [Hannig et al, 2010b] but not by using a mouthrinse [Hannig et al, 2010a]. In vitro (no pellicle): boxplots showing the reduction of loss of calcium and phosphate after the use of the three toothpastes compared to water.…”

Section: Discussionmentioning

confidence: 99%

Reduction of Erosion by Protein-Containing Toothpastes

Jager¹,

Vissink

Timmer³

et al. 2012

Caries Res

View full text Add to dashboard Cite

Aim: To assess the effect of protein-containing toothpastes on the progression of dental erosion in situ (with pellicle) and in vitro (without pellicle). Methods: A combined split-mouth (extraoral water or toothpaste brushing) and crossover (type of toothpaste) setup was used. Two protein-containing (high/low concentrations of colostrum) and one nonprotein (placebo) toothpaste were investigated. Sixteen volunteers wore intraoral appliances containing 2 human enamel samples on 3 afternoons for pellicle growth during 90 min. One enamel sample was brushed for 5 s with one of the three toothpastes and subsequently exposed to a slurry of the corresponding toothpaste for 2 min. The other sample was exposed to water. Both samples were subsequently exposed to citric acid (extraorally). Loss of calcium and inorganic phosphate were determined. The same sequence of exposures was applied to 16 enamel samples in an in vitro setup without pellicle. Results: With the in situ-formed pellicle, all toothpastes significantly reduced calcium loss compared to water brushing, although no significant differences were found among toothpastes (p = 0.073). For the loss of phosphate, a significant reduction could be found with the use of the high-protein toothpaste compared to the nonprotein toothpaste. Overall there were only slight differences between the toothpastes. Toothpaste effects were less clear in the in vitro experiment. Conclusion: The addition of proteins to toothpaste shows some promise for the prevention of erosion. Further research is needed to investigate the performance of the protein-containing toothpastes in longer in situ studies with regard to erosive wear.

show abstract

Efficacy of enzymatic mouth rinses for immobilisation of protective enzymes in the in situ pellicle

Cited by 18 publications

References 35 publications

Enzymes in the in‐situ pellicle of children with different caries activity

Enzymes in the in‐situ pellicle of children with different caries activity

Proteome and Peptidome of Human Acquired Enamel Pellicle on Deciduous Teeth

Reduction of Erosion by Protein-Containing Toothpastes

Contact Info

Product

Resources

About