Inhibition of ANGPT2 activates autophagy during hypertrophic scar formation via PI3K/AKT/mTOR pathway

Chen, Hongxin; Xu, Kai; Sun, Chao; Gui, Si; Wu, Juanjuan; Wang, Song

doi:10.1016/j.abd.2021.12.005

Cited by 6 publications

(4 citation statements)

References 48 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the dermis layer a crucial role is played by the large blood vessels for the delivery of nutrients to the cells, and new blood vessel formation is an essential event after inflammation or injuries. Fibroblasts, when appropriately stimulated, can release into their environment factors which are able to stimulate endothelial cells during the angiogenic process [ 27 ]. Our results demonstrated that SS administration indirectly supports the recruitment of molecular pathways leading to new blood vessel formation.…”

Section: Discussionmentioning

confidence: 99%

Snail Slime Extracted by a Cruelty Free Method Preserves Viability and Controls Inflammation Occurrence: A Focus on Fibroblasts

et al. 2023

View full text Add to dashboard Cite

Snail slime (SS) is a viscous secretion obtained from different snail species. SS composition is variable according to factors such as the extraction method. Even if several papers have been published regarding this topic, the molecular mechanisms at the base of SS biological effects remain unexplored. Thus, the aim of this study is to evaluate the capability of SS, extracted with the cruelty-free Muller method, to promote viability and angiogenesis processes and, in parallel, to counteract inflammation occurrence on skin cell populations. SS was administered to keratinocytes, macrophages and fibroblasts, then cell viability, through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, cytotoxicity by lactate dehydrogenase (LDH) assay, morphology by haematoxylin-eosin staining, gene and protein expression through real-time polymerase chain reaction (PCR) and Western blot, cell cycle phases by flow cytometry, and collagen secretion using an enzyme-linked immunosorbent assay (ELISA) test, were measured. Our results evidence SS capability to promote fibroblast viability and to trigger recovery mechanisms by activating the Erk protein. Moreover, an appreciable anti-inflammatory effect due to the significant reduction in cyclooxygenase-2 expression, and a positive modulation of new blood vessel formation demonstrated by increased Angiopoietin 1 gene expression and a higher matrix deposition (evidenced by the augmented amount of released collagen I) can be identified. This evidence led us to assume that the Muller method extracted-SS represents a valuable and promising natural product suitable for cosmetic and skin care formulations.

show abstract

Section: Discussionmentioning

confidence: 99%

Snail Slime Extracted by a Cruelty Free Method Preserves Viability and Controls Inflammation Occurrence: A Focus on Fibroblasts

et al. 2023

View full text Add to dashboard Cite

show abstract

“…It has been shown that the inhibition of ANGPT2 activates autophagy during hypertrophic scar formation through the PI3K/AKT/mTOR pathway ( 18 ). Autophagy plays a pivotal regulatory role in UC.…”

Section: Discussionmentioning

confidence: 99%

Silencing ANGPT2 alleviates ulcerative colitis by regulating autophagy-mediated NLRP3 inflammasome inactivation via the mTOR signaling pathway

Wang,

Huang,

Liu

et al. 2024

Braz J Med Biol Res

View full text Add to dashboard Cite

Ulcerative colitis (UC) is a difficult intestinal disease characterized by inflammation, and its mechanism is complex and diverse. Angiopoietin-like protein 2 (ANGPT2) plays an important regulatory role in inflammatory diseases. However, the role of ANGPT2 in UC has not been reported so far. After exploring the expression level of ANGPT2 in serum of UC patients, the reaction mechanism of ANGPT2 was investigated in dextran sodium sulfate (DSS)-induced UC mice. After ANGPT2 expression was suppressed, the clinical symptoms and pathological changes of UC mice were detected. Colonic infiltration, oxidative stress, and colonic mucosal barrier in UC mice were evaluated utilizing immunohistochemistry, immunofluorescence, and related kits. Finally, western blot was applied for the estimation of mTOR signaling pathway and NLRP3 inflammasome-related proteins. ANGPT2 silencing improved clinical symptoms and pathological changes, alleviated colonic inflammatory infiltration and oxidative stress, and maintained the colonic mucosal barrier in DSS-induced UC mice. The regulatory effect of ANGPT2 on UC disease might occur by regulating the mTOR signaling pathway and thus affecting autophagy-mediated NLRP3 inflammasome inactivation. ANGPT2 silencing alleviated UC by regulating autophagy-mediated NLRP3 inflammasome inactivation via the mTOR signaling pathway.

show abstract

“…During this process, damaged proteins or organelles are enclosed in autophagic vesicles and transported to lysosomes for degradation and recycling. While autophagy is constitutively active in all cell types under normal conditions, it is rapidly upregulated in response to stress 68 . LC3B‐II, a marker of autophagosomes, is present in the autophagosome membrane and undergoes degradation upon fusion with lysosomes.…”

Section: Autophagy Mitochondrial Pathwaymentioning

confidence: 99%

ZnO nanomaterials target mitochondrial apoptosis and mitochondrial autophagy pathways in cancer cells

Li,

et al. 2024

Cell Biochemistry & Function

View full text Add to dashboard Cite

In recent years, the application of engineering nanomaterials has significantly contributed to the development of various biomedical fields. Zinc oxide nanomaterials (ZnO NMts) have gained wide popularity due to their biocompatibility, unique physical and chemical properties, stability, and cost‐effectiveness for large‐scale production. They have emerged as potential materials for anticancer applications. This article provides a comprehensive review of the synthesis methods of ZnO NMts and highlights the advantages of combining ZnO NMts with anticancer drugs as a nano platform for cancer treatment. Additionally, the article briefly explains the mechanism of action of ZnO NMts in tumor cells, focusing on the mitochondrial pathways that target cell apoptosis and autophagy. It is observed that these pathways are primarily influenced by reactive oxygen species generated through oxidative stress. The article discusses the promising prospects of ZnO NMts combined with anticancer drugs in the field of cancer medicine and emphasizes the need for further in‐depth research on the mitochondrial apoptosis and mitochondrial autophagy pathways.

show abstract

Inhibition of ANGPT2 activates autophagy during hypertrophic scar formation via PI3K/AKT/mTOR pathway

Cited by 6 publications

References 48 publications

Snail Slime Extracted by a Cruelty Free Method Preserves Viability and Controls Inflammation Occurrence: A Focus on Fibroblasts

Snail Slime Extracted by a Cruelty Free Method Preserves Viability and Controls Inflammation Occurrence: A Focus on Fibroblasts

Silencing ANGPT2 alleviates ulcerative colitis by regulating autophagy-mediated NLRP3 inflammasome inactivation via the mTOR signaling pathway

ZnO nanomaterials target mitochondrial apoptosis and mitochondrial autophagy pathways in cancer cells

Contact Info

Product

Resources

About