Meat Safety

Dasenaki, Marilena E.; Τhomaidis, Νikolaos S.

doi:10.1016/b978-0-08-100694-8.00018-2

Cited by 7 publications

(7 citation statements)

References 43 publications

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“…Internal NAT addition has also been used for LC/MS‐based quantitation of small molecules in various tissues and in postmortem blood . A recent multiplexed MRM study used standard addition, for the quantitative analysis of 115 veterinary drugs in a variety of food matrices.…”

Section: Introductionmentioning

confidence: 99%

ExSTA: External Standard Addition Method for Accurate High‐Throughput Quantitation in Targeted Proteomics Experiments

Mohammed

Pan

Zhang³

et al. 2017

Proteomics Clinical Apps

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PurposeTargeted proteomics using MRM with stable‐isotope‐labeled internal‐standard (SIS) peptides is the current method of choice for protein quantitation in complex biological matrices. Better quantitation can be achieved with the internal standard‐addition method, where successive increments of synthesized natural form (NAT) of the endogenous analyte are added to each sample, a response curve is generated, and the endogenous concentration is determined at the x‐intercept. Internal NAT‐addition, however, requires multiple analyses of each sample, resulting in increased sample consumption and analysis time.Experimental designTo compare the following three methods, an MRM assay for 34 high‐to‐moderate abundance human plasma proteins is used: classical internal SIS‐addition, internal NAT‐addition, and external NAT‐addition—generated in buffer using NAT and SIS peptides. Using endogenous‐free chicken plasma, the accuracy is also evaluated.ResultsThe internal NAT‐addition outperforms the other two in precision and accuracy. However, the curves derived by internal vs. external NAT‐addition differ by only ≈3.8% in slope, providing comparable accuracies and precision with good CV values.Conclusions and clinical relevanceWhile the internal NAT‐addition method may be “ideal”, this new external NAT‐addition can be used to determine the concentration of high‐to‐moderate abundance endogenous plasma proteins, providing a robust and cost‐effective alternative for clinical analyses or other high‐throughput applications.

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Section: Introductionmentioning

confidence: 99%

ExSTA: External Standard Addition Method for Accurate High‐Throughput Quantitation in Targeted Proteomics Experiments

Mohammed

Pan

Zhang³

et al. 2017

Proteomics Clinical Apps

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“…An LC-electrospray ionization mass spectrometry (ESI-MS) system (Shimadzu LCMS-2020; Japan) was used to confirm the identity of the analytes in the extract. The chromatographic conditions were the same used for the DAD analysis, while for ESI-MS, they were based on a study by Dasenaki and Thomaidis, 12 as follows: positive ESI mode; interface temperature, 350 o C; heat block, 200 o C; desolvation line (DL) temperature, 250 o C; nebulizing gas flow, 1.5 L min -1 ; drying gas flow, 15 L min -1 ; and capillary voltage, 4500 V.…”

Section: Sample Analysismentioning

confidence: 99%

“…2,[8][9][10] These antibiotics have been widely used for prophylactic and therapeutic purposes, to treat bacterial infections, as well as used in the veterinary practice to promote animal growth. [10][11][12][13][14][15][16][17] The misuse of antibiotics, such as overdoses or insufficient time gaps between the drug application and the honey commercialization, may result in drug residues and, therefore, making their consumption inappropriate for humans. 13 The presence of such residues in honey entails risks to the consumer's health, may cause depreciation of the product in the international market, gives rise to adverse effects in hypersensitive individuals, such as allergic reactions, as well as carcinogenicity and development of resistance in pathogenic bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…30,32 However, the extraction and pre-concentration steps of samples, such as in liquid-liquid extraction (LLE), 17 usually require long times of sample preparation and large amounts of sample and solvents, as well as present limitations for obtaining high enrichment factor and recovery. 12,33 On the other way, the solid phase extraction, which was used in several works, 6,7,13,14,16,21,23 despite its advantages demands in general long times of sample preparation, brings high costs of commercially available cartridges and manifolds and, eventually, difficulties for selecting the more appropriate solvent for the desired preparation. 34 In recent years, microextraction techniques have been successfully applied to the analysis of antibiotics and other types of analytes in honey.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Method Based on DLLME and UFLC-DAD for the Determination of Antibiotics in Honey Samples and the Study of Their Degradation Kinetics

Santana¹,

Santana²,

Pereira³

2018

J. Braz. Chem. Soc.

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In this work, a method was developed, based on dispersive liquid-liquid microextraction (DLLME) and analysis by ultra-fast liquid chromatography coupled to diode array detector (UFLC-DAD), to simultaneously determine ten antibiotics of two different classes in honey, as well as to evaluate their degradation kinetics in this matrix. The extraction was optimized by a Box-Behnken design and the method provided good linearity and precision, limits of detection (LODs) in the range of 3.1-6.8 µg kg-1 and recoveries between 82.9 and 105.7%. The decay velocities along the storage period have followed a first order model for nine antibiotics, while one has fitted a zero order model. In the analysis of 33 samples, sulfadimethoxine and tetracycline were found in four samples of the same year and type of blooming, freshly produced in different places, at concentrations, respectively, below and 79% higher than the established by the Brazilian legislation. This suggests an application of those drugs to the beehives, probably shortly before the samples were taken.

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“…Various classes of veterinary medications are widely and legally used to treat and prevent diseases in livestock . In principle, all drugs administered to livestock can leave residues in edible food products, including meat, milk, eggs, liver, kidney, and fat .…”

Section: Introductionmentioning

confidence: 99%

Determination of residual levels of naloxone, yohimbine, thiophanate, and altrenogest in porcine muscle using QuEChERS with liquid chromatography and triple quadrupole mass spectrometry

Zhang

Park

Kim

et al. 2016

J of Separation Science

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A quick, easy, cheap, effective, rugged, and safe QuEChERS (method) was used for the simultaneous detection of four veterinary drug residues, namely naloxone, yohimbine, thiophanate, and altrenogest, in porcine muscle, using liquid chromatography with electrospray ionization triple quadrupole tandem mass spectrometry. Because of the unavailability of a suitable internal standard, matrix-matched calibrations were used for quantification, with determination coefficients ≥ 0.9542. The accuracy (expressed as recovery %) ranged from 60.53 to 83.25%, and the intra- and interday precisions (expressed as relative standard deviations) were <12%. The limits of quantification were 5, 0.5, 2, and 5 ng/g for naloxone, yohimbine, thiophanate, and altrenogest, respectively. Samples purchased from local markets in Seoul, Republic of Korea, revealed no traces of the target analytes. The developed method described herein is sensitive and reliable and can be applied to quantify the tested veterinary drugs in animal tissues.

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Meat Safety

Cited by 7 publications

References 43 publications

ExSTA: External Standard Addition Method for Accurate High‐Throughput Quantitation in Targeted Proteomics Experiments

ExSTA: External Standard Addition Method for Accurate High‐Throughput Quantitation in Targeted Proteomics Experiments

Development of a Method Based on DLLME and UFLC-DAD for the Determination of Antibiotics in Honey Samples and the Study of Their Degradation Kinetics

Determination of residual levels of naloxone, yohimbine, thiophanate, and altrenogest in porcine muscle using QuEChERS with liquid chromatography and triple quadrupole mass spectrometry

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