The effect of varying LED light sources and influent carbon/nitrogen ratios on treatment of synthetic sanitary sewage using Chlorella vulgaris

Xu, Bing; Cheng, Pu; Chen, Yan; Pei, Haiyan; Hu, Wenrong

doi:10.1007/s11274-013-1292-6

Cited by 24 publications

(6 citation statements)

References 29 publications

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“…The W or full spectrum (λ = 400-700 nm) tends to cause photoinhibition, inducing cell damage and reducing the overall time of stationary phase as compared to R ( Figure 2). Since the cumulative wavelength of red in R is lower than that in W, it may prevent photoinhibition and consequently lead to higher growth and overall biomass [39]. Similar results have been reported for Chlorella sp.…”

Section: Growth Curve Analysis Of P Tricornutum During Red Light Shiftsupporting

confidence: 81%

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

et al. 2020

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Marine water diatom Phaeodactylum tricornutum is a photosynthetic organism that is known to respond to the changing light environment and adapt to different temperatures to prevent photoinhibition and maintain its metabolic functions. The objective of the present study was to test whether light shift variations in different growth phases impact the growth and lipid metabolism of P. tricornutum. Thus, we investigated R exposure in different growth phases to find the most effective light shift condition. The results showed that substituting white light (W) by red light (R) under autotrophic conditions, a condition called red shift (RS), increased biomass and lipid content compared to levels found under continuous W or R exposure alone. We observed an increase by 2-fold biomass and 2.3-fold lipid content in RS as compared to W. No significant change was observed in the morphology of lipid droplets, but the fatty acid (FA) composition was altered. Specifically, polyunsaturated FAs were increased, whereas monounsaturated FAs decreased in P. tricornutum grown in RS compared to W control. Therefore, we propose that a light shift during the beginning of the stationary phase is a low-cost cultivation strategy to boost the total biomass and lipids in P. tricornutum.Appl. Sci. 2020, 10, 2531 2 of 18 for the high production of desired bioactive molecules. Therefore, light should be provided with specific spectral components, intensity, and duration. Multiple ecophysiological studies have been published reporting on the distinct behaviors of different diatoms and algae in response to variations in light [4,5]. It has been shown that light quality has an impact on chloroplast migration and on the light acclimation reactions of photosynthesis [6,7]. Red light has been linked to stimulating growth, ethylene production [8], lipid accumulation [7], and increasing thylakoid stacking [6]. have suggested the involvement of phytochromes in increasing the intracellular nitrate content on exposure to red light for short periods altering the overall cellular nitrate content. Recent discoveries in genomics have revealed exciting information on phytochromes, blue-light sensing cryptochromes, and aureochromes [10]. In a study by Jungandreas et al. [11], it has been reported that P. tricornutum cells acclimated to both red and blue light have comparable metabolite profiles, but drastic changes in metabolites and carbon partitioning was observed under red to blue light shift. In addition, various interdisciplinary studies have shown that light perceived by the red-light receptor (phytochromes) can regulate nutrient metabolism, cellular events, and signaling cascades [12]. These discoveries are still in progress and much remains unknown, but these studies can be explored for application-based diatom experiments such as developing low-cost cultivation technology or efficient bioreactors.In both culture medium and natural water bodies, light (intensity, distribution, photoperiod) may vary and will not behave similar to the dissolved salts or nu...

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Section: Growth Curve Analysis Of P Tricornutum During Red Light Shiftsupporting

confidence: 81%

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

et al. 2020

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“…These results coincide with those of other authors that found that red light promoted the highest growth rate and produced the highest biomass in C. Vulgaris compared to the other wavelength distributions. Wang et al.…”

Section: Resultssupporting

confidence: 93%

Impact of light quality on a native Louisiana Chlorella vulgaris/Leptolyngbya sp. co‐culture

Barnett

Foy

Malone

et al. 2017

Engineering in Life Sciences

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Impact of light quality on a native Louisiana Chlorella vulgaris/Leptolyngbya sp. co-cultureLight effect on cultures of microalgae has been studied mainly on single species cultures. Cyanobacteria have photosynthetic pigments that can capture photons of wavelengths not available to chlorophylls. A native Louisiana microalgae (Chlorella vulgaris) and cyanobacteria (Leptolyngbya sp.) co-culture was used to study the effects of light quality (blue-467 nm, green-522 nm, red-640 nm and white-narrow peak at 450 nm and a broad range with a peak at 550 nm) at two irradiance levels (80 and 400 μmol m −2 s −1 ) on the growth, species composition, biomass productivity, lipid content and chlorophyll-a production. The co-culture shifted from a microalgae dominant culture to a cyanobacteria culture at 80 μmol m −2 s −1 . The highest growth for the cyanobacteria was observed at 80 μmol μmol m −2 s −1 and for the microalgae at 400 μmol m −2 s −1 . Red light at 400 μmol m −2 s −1 had the highest growth rate (0.41 d −1 ), biomass (913 mg L −1 ) and biomass productivity (95 mg L −1 d −1 ). Lipid content was similar between all light colors. Green light had the highest chlorophyll-a content (1649 μg/L). These results can be used to control the species composition of mixed cultures while maintaining their productivity.

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“…In addition, more photons are usually released by LEDs emitting at longer wavelengths (e.g., red), resulting in higher PPFD/W ratios than for LEDs emitting at shorter wavelengths (e.g., blue) [1], because blue photons are more energetic than red photons. Specifically, red (l e = 660 nm) LEDs can emit double the number of photons that blue LEDs emit, whereas green LEDs emit approximately three times fewer photons than red LEDs do [5,18,[58][59][60]. At face value, these results suggest that 660-nm red LEDs are able to sustain biomass growth with the highest energy efficiency [1].…”

Section: Leds For Microalgal Productionmentioning

confidence: 95%

“…8 wavelengths are located below, between, or above these ranges. Exposure of microalgae to accessory wavelengths (l e % 500-630 nm) alone consistently leads to lower biomass production compared to growth under either blue (l e % 430-470 nm) or red (l e % 660 nm) LEDs [6,12,15,[18][19][20][21][22][23][24]. Green (l e % 525-550 nm) LEDs were often found to be highly unsuitable for microalgae if used without additional light sources (Table S1 in the supplementary material online) [1,22,23,25,26].…”

Section: Reviewmentioning

confidence: 99%

Light emitting diodes (LEDs) applied to microalgal production

Schulze¹,

Barreira²,

Pereira³

et al. 2014

Trends in Biotechnology

289

139

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Light-emitting diodes (LEDs) will become one of the world's most important light sources and their integration in microalgal production systems (photobioreactors) needs to be considered. LEDs can improve the quality and quantity of microalgal biomass when applied during specific growth phases. However, microalgae need a balanced mix of wavelengths for normal growth, and respond to light differently according to the pigments acquired or lost during their evolutionary history. This review highlights recently published results on the effect of LEDs on microalgal physiology and biochemistry and how this knowledge can be applied in selecting different LEDs with specific technical properties for regulating biomass production by microalgae belonging to diverse taxonomic groups.

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The effect of varying LED light sources and influent carbon/nitrogen ratios on treatment of synthetic sanitary sewage using Chlorella vulgaris

Cited by 24 publications

References 29 publications

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

Impact of light quality on a native Louisiana Chlorella vulgaris/Leptolyngbya sp. co‐culture

Light emitting diodes (LEDs) applied to microalgal production

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