A robust KASP marker for selection of four pairs of linked leaf rust and stripe rust resistance genes introgressed on chromosome arm 5DS from different wheat genomes

Bansal, Mitaly; Adamski, Nikolai M; Toor, Puneet Inder; Kaur, Satinder; Sharma, Achla; Srivastava, Puja; Bansal, Urmil; Uauy, Cristóbal; Chhuneja, Parveen

doi:10.1007/s11033-021-06525-4

Cited by 3 publications

(2 citation statements)

References 40 publications

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“…Markers have been developed for many ASR genes conditioning resistance to leaf rust; Lr23 [ 40 ], Lr24 [ 41 ], Lr42 [ 42 ], Lr52 [ 21 ], Lr53 [ 43 ], Lr57 [ 44 ], Lr76 [ 44 ], Lr80 [ 14 ] and APR genes Lr34 [ 45 ], Lr48 [ 23 ], Lr49 [ 46 ], Lr67 [ 47 ] and Lr68 [ 48 ]. These ASR and APR genes can be pyramided in different combinations to combat evolution in Pt populations.…”

Section: Discussionmentioning

confidence: 99%

Discovery of the New Leaf Rust Resistance Gene Lr82 in Wheat: Molecular Mapping and Marker Development

Bariana

Babu

Forrest

et al. 2022

Genes

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Breeding for leaf rust resistance has been successful worldwide and is underpinned by the discovery and characterisation of genetically diverse sources of resistance. An English scientist, Arthur Watkins, collected pre-Green Revolution wheat genotypes from 33 locations worldwide in the early part of the 20th Century and this collection is now referred to as the ‘Watkins Collection’. A common wheat genotype, Aus27352 from Yugoslavia, showed resistance to currently predominating Australian pathotypes of the wheat leaf rust pathogen. We crossed Aus27352 with a leaf rust susceptible wheat selection Avocet S and a recombinant inbred line (RIL) F6 population of 200 lines was generated. Initial screening at F3 generation showed monogenic segregation for seedling response to leaf rust in Aus27352. These results were confirmed by screening the Aus27352/Avocet S RIL population. The underlying locus was temporarily named LrAW2. Bulked segregant analysis using the 90K Infinium SNP array located LrAW2 in the long arm of chromosome 2B. Tests with molecular markers linked to two leaf rust resistance genes, Lr50 and Lr58, previously located in chromosome 2B, indicated the uniqueness of LrAW2 and it was formally designated Lr82. Kompetitive allele-specific polymerase chain reaction assays were developed for Lr82-linked SNPs. KASP_22131 mapped 0.8 cM proximal to Lr82 and KASP_11333 was placed 1.2 cM distal to this locus. KASP_22131 showed 91% polymorphism among a set of 89 Australian wheat cultivars. We recommend the use of KASP_22131 for marker assisted pyramiding of Lr82 in breeding programs following polymorphism check on parents.

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Section: Discussionmentioning

confidence: 99%

Discovery of the New Leaf Rust Resistance Gene Lr82 in Wheat: Molecular Mapping and Marker Development

Bariana

Babu

Forrest

et al. 2022

Genes

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“…Additionally, precision genome editing, using the CRISPR-Cas9A technique is rapidly becoming popular. In wheat, it has been used to improve drought tolerance ( 157 ) and herbicide tolerance ( 158 ). As the acceptance of the use of CRISPR-Cas9 increases, it can become the method of choice for mirror alien genes of interest to have in wheat.…”

Section: Benefits Of Traditional and Modern Breeding Methodologies Fo...mentioning

confidence: 99%

Alien introgression to wheat for food security: functional and nutritional quality for novel products under climate change

Johansson,

Lan,

Olalekan

et al. 2024

Front. Nutr.

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Crop yield and quality has increased globally during recent decades due to plant breeding, resulting in improved food security. However, climate change and shifts in human dietary habits and preferences display novel pressure on crop production to deliver enough quantity and quality to secure food for future generations. This review paper describes the current state-of-the-art and presents innovative approaches related to alien introgressions into wheat, focusing on aspects related to quality, functional characteristics, nutritional attributes, and development of novel food products. The benefits and opportunities that the novel and traditional plant breeding methods contribute to using alien germplasm in plant breeding are also discussed. In principle, gene introgressions from rye have been the most widely utilized alien gene source for wheat. Furthermore, the incorporation of novel resistance genes toward diseases and pests have been the most transferred type of genes into the wheat genome. The incorporation of novel resistance genes toward diseases and pests into the wheat genome is important in breeding for increased food security. Alien introgressions to wheat from e.g. rye and Aegilops spp. have also contributed to improved nutritional and functional quality. Recent studies have shown that introgressions to wheat of genes from chromosome 3 in rye have an impact on both yield, nutritional and functional quality, and quality stability during drought treatment, another character of high importance for food security under climate change scenarios. Additionally, the introgression of alien genes into wheat has the potential to improve the nutritional profiles of future food products, by contributing higher minerals levels or lower levels of anti-nutritional compounds into e.g., plant-based products substituting animal-based food alternatives. To conclude, the present review paper highlights great opportunities and shows a few examples of how food security and functional-nutritional quality in traditional and novel wheat products can be improved by the use of genes from alien sources, such as rye and other relatives to wheat. Novel and upcoming plant breeding methods such as genome-wide association studies, gene editing, genomic selection and speed breeding, have the potential to complement traditional technologies to keep pace with climate change and consumer eating habits.

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Postulation of leaf rust resistance genes in Indian and exotic wheat germplasm using near-isogenic lines (NILs) and molecular markers

Kaur,

Bala

et al. 2023

Crop Protection

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A robust KASP marker for selection of four pairs of linked leaf rust and stripe rust resistance genes introgressed on chromosome arm 5DS from different wheat genomes

Cited by 3 publications

References 40 publications

Discovery of the New Leaf Rust Resistance Gene Lr82 in Wheat: Molecular Mapping and Marker Development

Discovery of the New Leaf Rust Resistance Gene Lr82 in Wheat: Molecular Mapping and Marker Development

Alien introgression to wheat for food security: functional and nutritional quality for novel products under climate change

Postulation of leaf rust resistance genes in Indian and exotic wheat germplasm using near-isogenic lines (NILs) and molecular markers

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