Impacts of Carbon and Flooding on Soil Microbial Communities: Phospholipid Fatty Acid Profiles and Substrate Utilization Patterns

Bossio, Deborah A.; Scow, Kate M.

doi:10.1007/s002489900082

Cited by 1,145 publications

(613 citation statements)

References 34 publications

Supporting

Mentioning

573

Contrasting

Unclassified

Order By: Relevance

“…Soil microbial community composition was analyzed using the phospholipid fatty acid (PLFA) method following Bossio and Scow (1998). Concentrations of individual PLFAs were calculated based on 19:0 internal standard concentrations; the samples were analyzed on a MIDI Sherlock microbial identification system 6.0 (microbial ID, Inc. Newark, DE 19713).…”

Section: Methodsmentioning

confidence: 99%

Effects of temperature, soil substrate, and microbial community on carbon mineralization across three climatically contrasting forest sites

Tang

Sun

Luo

et al. 2017

Ecology and Evolution

View full text Add to dashboard Cite

How biotic and abiotic factors influence soil carbon (C) mineralization rate (R S) has recently emerged as one of the focal interests in ecological studies. To determine the relative effects of temperature, soil substrate and microbial community on R s, we conducted a laboratory experiment involving reciprocal microbial inoculations of three zonal forest soils, and measured R S over a 61‐day period at three temperatures (5, 15, and 25°C). Results show that both R s and the cumulative emission of C (R cum), normalized to per unit soil organic C (SOC), were significantly affected by incubation temperature, soil substrate, microbial inoculum treatment, and their interactions (p < .05). Overall, the incubation temperature had the strongest effect on the R S; at given temperatures, soil substrate, microbial inoculum treatment, and their interaction all significantly affected both R s (p < .001) and R cum (p ≤ .01), but the effect of soil substrate was much stronger than others. There was no consistent pattern of thermal adaptation in microbial decomposition of SOC in the reciprocal inoculations. Moreover, when different sources of microbial inocula were introduced to the same soil substrate, the microbial community structure converged with incubation without altering the overall soil enzyme activities; when different types of soil substrate were inoculated with the same sources of microbial inocula, both the microbial community structure and soil enzyme activities diverged. Overall, temperature plays a predominant role in affecting R s and R cum, while soil substrate determines the mineralizable SOC under given conditions. The role of microbial community in driving SOC mineralization is weaker than that of climate and soil substrate, because soil microbial community is both affected, and adapts to, climatic factors and soil matrix.

show abstract

Section: Methodsmentioning

confidence: 99%

Effects of temperature, soil substrate, and microbial community on carbon mineralization across three climatically contrasting forest sites

Tang

Sun

Luo

et al. 2017

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…Soil PLFAs were extracted using the procedure of Bossio and Scow (1998) and was determined using an Agilent 6890N Gas Chromatograph with MIDI peak identification software ( …”

Section: Soil Sampling and Analysismentioning

confidence: 99%

Responses of absolute and specific soil enzyme activities to long term additions of organic and mineral fertilizer

Zhang

Dong

Dai

et al. 2015

Science of The Total Environment

144

View full text Add to dashboard Cite

• Acid phosphatase activity was negatively correlated with soil pH and P content.• βG, NAG, and LAP activities were positively correlated with SOC and Total N contents.• The four enzyme activities were significantly higher under manure than NPK fertilizer.• Enzyme activities were positively correlated with actinomycete and G + bacterium.• We recommend reducing P fertilizer application rates to subtropical paddy soils. G R A P H I C A L A B S T R A C Ta b s t r a c t a r t i c l e i n f o Long-term phosphorus (P) and nitrogen (N) applications may seriously affect soil microbial activity. A long-term field fertilizer application trial was established on reddish paddy soils in the subtropical region of southern China in 1998. We assessed the effects of swine manure and seven different rates or ratios of NPK fertilizer treatments on (1) the absolute and specific enzyme activities per unit of soil organic carbon (SOC) or microbial biomass carbon (MBC) involved in C, N, and P transformations and (2) their relationships with soil environmental factors and soil microbial community structures. The results showed that manure applications led to increases in the absolute and specific activities of soil β-1,4-glucosidase(βG), β-1,4-N-acetylglucosaminidase (NAG), and leucine aminopeptidase (LAP). The absolute and specific acid phosphatase (AP) activities decreased as mineral P fertilizer application rates and ratios increased. Redundancy analysis (RDA) showed that there were negative correlations between absolute and specific AP activities, pH, and total P contents, while there were positive correlations between soil absolute and specific βG, NAG, and LAP enzyme activities, and SOC and total N contents. RDA showed that the contents of actinomycete and Gram-positive bacterium PLFA biomarkers are more closely related to the absolute and specific enzyme activities than the other PLFA biomarkers (P b 0.01). Our results suggest that both the absolute and specific enzyme activities could be used as sensitive soil quality indicators that provide useful linkages with the microbial community structures and environmental factors. To maintain microbial activity and to minimize environmental impacts, P should be applied as a combination of inorganic and organic forms, and total P fertilizer application rates to subtropical paddy soils should not exceed 44 kg P ha −1 year −1 .

show abstract

“…In addition to the standard GN plates, a set of carbon sources relevant to hydrocarbon degradation was used in order to determine whether this would improve the sensitivity or selectivity of the sole carbon source utilization pro®ling. Previous work has shown that addition of ecologically-relevant carbon sources to Biolog GN sources can improve the discrimination between microbial communities (Campbell et al 1997). Secondly, phospholipid fatty acid (PLFA) pro®ling of the microbial community using phospholipids extracted directly from the soil is a well characterized technique for providing information on the microbial community structure that is not based on culturing of microbes (Tunlid and White 1992;Zelles 1999).…”

Section: Introductionmentioning

confidence: 99%

Microbial communities in different soil types do not converge after diesel contamination

2002

View full text Add to dashboard Cite

Impacts of Carbon and Flooding on Soil Microbial Communities: Phospholipid Fatty Acid Profiles and Substrate Utilization Patterns

Cited by 1,145 publications

References 34 publications

Effects of temperature, soil substrate, and microbial community on carbon mineralization across three climatically contrasting forest sites

Effects of temperature, soil substrate, and microbial community on carbon mineralization across three climatically contrasting forest sites

Responses of absolute and specific soil enzyme activities to long term additions of organic and mineral fertilizer

Microbial communities in different soil types do not converge after diesel contamination

Contact Info

Product

Resources

About