MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes

Steuernagel, Burkhard; Witek, Kamil; Jones, Jonathan D. G.; Wulff, Brande B. H.

doi:10.1007/978-1-4939-7249-4_19

Cited by 25 publications

(11 citation statements)

References 14 publications

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“…In line with these data, research will have to fully consider comparative genomics within the Ma locus to identify the resistance determinants (sequences and/or motifs) and in particular, those involved in the resistance specificity. Methods and model studies published recently open the way in this objective [75][76][77]. The noteworthy C-terminal part of the gene with a huge post-LRR region (=5 repeated PL domains) makes it the longest R gene ever cloned.…”

Section: Discussionmentioning

confidence: 99%

Deciphering Resistance to Root-Knot Nematodes in Prunus for Rootstock Breeding: Sources, Genetics and Characterization of the Ma Locus

Esmenjaud¹

2021

Horticulturae

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Root-knot nematode (RKN) species are predominant pests of crops, attacking stone fruit crops Prunus spp. under Mediterranean climate conditions worldwide. Natural resistance for rootstock breeding is a control method that is gaining interest as an alternative to the highly toxic nematicides. This review first reports an outline of the root-knot nematodes parasitizing stone fruit crops and the Prunus species and rootstocks. It then describes the main sources of resistance detected among the Prunus germplasm and focuses on the major resistance genes identified and their characteristics (spectrum, durability, histological mechanism, effect of temperature, interaction with other pests and diseases, etc.). In peach, besides the RMia reference gene, the new genes PkMi and Mf, also located on chromosome 2, need to be characterized regarding their spectrum and relationship. The two other Prunus reference genes, Ma from plum (complete spectrum) and RMja from almond (more restricted spectrum), are orthologs that belong to a TIR-NB-LRR (TNL) cluster on chromosome 7. The review finally summarizes the positional cloning of the Ma gene and the characterization of its unique TNL structure, encompassing a five-times repeated post-LRR domain. Deciphering how this structure is functionally involved in Ma’s remarkable biological properties is a real challenge for the future.

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Section: Discussionmentioning

confidence: 99%

Deciphering Resistance to Root-Knot Nematodes in Prunus for Rootstock Breeding: Sources, Genetics and Characterization of the Ma Locus

Esmenjaud¹

2021

Horticulturae

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“…Illumina libraries with an average insert size of 700□bp were enriched by Arbor Biosciences, Michigan, USA, as previously described 26 , and sequenced on an Illumina HiSeq with either 150 or 250 PE reads at Novogene, China to generate an average of 3.82 Gb per accession (Table S2). The raw reads were trimmed using Trimmomatic v0.2 27 and de novo assembled with the CLC Assembly Cell (http://www.clcbio.com/products/clc-assembly-cell/) using word size (-w□=□64) with standard parameters.…”

Section: Methodsmentioning

confidence: 99%

A wheat kinase and immune receptor form the host-specificity barrier against the blast fungus

Arora

Steed

Goddard

et al. 2022

Preprint

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Since emerging in Brazil in 1985, wheat blast has spread throughout South America and recently appeared in Bangladesh and Zambia. We show that two wheat resistance genes, Rwt3 and Rwt4, acting as host-specificity barriers against non-Triticum blast pathotypes encode a nucleotide-binding leucine-rich repeat immune receptor and a tandem kinase, respectively. Molecular isolation of these genes allowed us to develop assays that will ensure the inclusion of these two genes in the wheat cultivars to forestall the recurrence of blast host jumps.

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“…4). Another promising option would be to integrate genomic selection with rapid disease-resistance gene discovery and cloning technologies 48,49 . While marker-assisted selection can be used to transfer resistance genes with large effects, coupling the approach with genomic selection could help to accumulate and maintain minor gene variants that contribute to effective resistance.…”

Section: Review Articlementioning

confidence: 99%