Liquid chromatography tandem mass spectrometry method for determination of bisoprolol in human plasma using d5‐bisoprolol as the internal standard

Liu, Gangyi; Wang, Wei; Jia, Jingying; Lü, Chuan; Liu, Yanmei; Mengqi, Zhang; Liu, Yun; Li, Shuijun; Chen, Yu

doi:10.1002/bmc.1329

Cited by 9 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Protein precipitation technique was applied for the separation of bisoprolol in human plasma, with a recovery rate of about 94.03, 100.3 and 96.29% for 1.5, 40 and 80 ng/mL of bisoprolol, respectively. After analyzing the samples no interference normally present in plasma was detected [13].…”

Section: Protein Precipitation Techniquementioning

confidence: 99%

“…These methods include liquid chromatography coupled with mass spectrometry (LC-MS/MS) [7][8][9][10][11][12][13][14][15][16][17], liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS) [18,19], gas chromatography coupled with mass spectrometry (GC-MS) [3,[20][21][22], high performance liquid chromatography (HPLC) [23,24], high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) [25,26], high performance liquid chromatography with fluorescence detection (HPLC-FLD) [2,27,28], high performance liquid chromatography coupled with ultraviolet detection (HPLC-UV) [29], reversed-phase ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS) [30].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

Corina¹,

Hăncianu²,

Agoroaei³

et al. 2015

Acta Medica Marisiensis

View full text Add to dashboard Cite

Arterial hypertension is a complex disease with many serious complications, representing a leading cause of mortality. Selective beta-blockers such as metoprolol and bisoprolol are frequently used in the management of hypertension. Numerous analytical methods have been developed for the determination of these substances in biological fluids, such as liquid chromatography coupled with mass spectrometry, gas chromatography coupled with mass spectrometry, high performance liquid chromatography. Due to the complex composition of biological fluids a biological sample pre-treatment before the use of the method for quantitative determination is required in order to remove proteins and potential interferences. The most commonly used methods for processing biological samples containing metoprolol and bisoprolol were identified through a thorough literature search using PubMed, ScienceDirect, and Willey Journals databases. Articles published between years 2005-2015 were reviewed. Protein precipitation, liquid-liquid extraction and solid phase extraction are the main techniques for the extraction of these drugs from plasma, serum, whole blood and urine samples. In addition, numerous other techniques have been developed for the preparation of biological samples, such as dispersive liquid-liquid microextraction, carrier-mediated liquid phase microextraction, hollow fiber-protected liquid phase microextraction, on-line molecularly imprinted solid phase extraction. The analysis of metoprolol and bisoprolol in human plasma, urine and other biological fluids provides important information in clinical and toxicological trials, thus requiring the application of appropriate extraction techniques for the detection of these antihypertensive substances at nanogram and picogram levels.

show abstract

Section: Protein Precipitation Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

Corina¹,

Hăncianu²,

Agoroaei³

et al. 2015

Acta Medica Marisiensis

View full text Add to dashboard Cite

show abstract

“…Recently, Liu et al reported a liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the quantitation of bisoprolol in human plasma with a linearity range of 0.5-100 ng/ml. Plasma aliquots spiked with bisoprolol were processed using simple protein precipitation extraction method [5] followed by chromatographic separation of bisoprolol achieved on Capcell Pak C18 MG III column (100 mm × 2.0 mm, 5 μ), using gradient elution. Similarly, Hemavanthi G. et al reported an LC-electrospray ionization (ESI)-MS for determination of bisoprolol and triamterene in human plasma.…”

Section: Introductionmentioning

confidence: 99%

A HPLC-MS/MS Method Development and Validation for the Simultaneous Determination of Bisoprolol and Enalapril in the Present of Enalaprilat in Human Plasma

et al. 2018

View full text Add to dashboard Cite

Objective: A highly specific, sensitive and rapid HPLC-MS/MS method has been developed and validated for the simultaneous quantification of bisoprolol and enalapril in the present of enalaprilat in human plasma.Methods: Analytes were extracted from plasma using a protein precipitation extraction method. Chromatography was achieved on Discovery C18, 50 × 2.1 mm, 5 μm column. Samples were chromatographed in a gradient mode (eluent A (acetonitrile-water–formic acid, 5: 95: 0.1 v/v), eluent B (acetonitrile–formic acid, 100: 0.1 v/v)). The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and to 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.400 ml/min into the mass spectrometer ESI chamber. The sample volume was 5 μl.Results: The total chromatographic run time was 2.0 min and the elution of bisoprolol, enalapril, enalaprilat and IS (verapamil) occurred at ~1.01, 1.03, 0.96 and 1.09 min, respectively. A linear response function was established at 0.5-50 ng/ml for bisoprolol fumarate, 2-200 ng/ml for enalapril maleate, 1-100 ng/ml for enalaprilat dehydrate in human plasma. The intraday and interday accuracy and precisions were in the range of 0.311 %-0.647 % and 0.364 %-0.572 % for bisoprolol, 0.321 %-0.747 % and 0.390 %-0.673 % for enalapril, 0.221 %-0.547 % and 0.264 %-0.773 % for enalaprilat, respectively.Conclusion: A new rapid method was developed for simultaneous determination of bisoprolol and enalapril in the present of enalaprilat in human plasma. The method was strictly validated according to the ICH guidelines. The information thus obtained from the study can be used for the full pharmacokinetic profiling in individuals.

show abstract

“…Recently, Liu et al reported a liquid chromatographytandem mass spectrometric (LC-MS/MS) method for the quantitation of bisoprolol in human plasma with a linearity range of 0.5-100 ng/ml. Plasma aliquots spiked with bisoprolol were processed using simple protein precipitation extraction method [3] followed by chromatographic separation of bisoprolol achieved on Capcell Pak C 18 MG III column (100 mm × 2.0 mm, 5 µ), using gradient elution.…”

Section: Introductionmentioning

confidence: 99%

Sensitive LC-MS/MS Method for the Simultaneous Determination of Bisoprolol and Triamterene in human plasma

Hemavathi

Hipparagi

2017

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: A highly sensitive, specific, and rapid liquid chromatography-electrospray ionization-mass spectrometry (MS)/MS method has been developed and validated for the simultaneous quantification of bisoprolol and triamterene in human plasma using metoprolol as an internal standard (IS) as per regulatory guidelines.Methods: Both the analytes and IS were extracted from plasma using a protein precipitation extraction method. Chromatography was achieved on Welchrom XB C18, 50 mm×4.6 mm, 5 µm column using an isocratic mobile phase (2 mM ammonium formate acetonitrile, 70:30 v/v) at a flow rate of 0.60 ml/minute.Results: The total chromatographic run time was 3.5 minute and the elution of bisoprolol, triamterene, and IS occurred at ~2.57, 1.30 and 1.57 minute, respectively. A linear response function was established at 2.04-210 ng/ml for both the analytes in human plasma. The intra- and inter-day accuracy and precisions were in the range of 1.12-7.87 and 1.26-6.36%; 1.46-6.13 and 1.65-7.34% for bisoprolol and triamterene, respectively.Conclusion: A new robust method was developed for simultaneous determination of Bisoprolol and Triamterene in human plasma. The method was strictly validated according to the ICH [1] guidelines. The information thus obtained from the study can be used for the full pharmacokinetic profiling in individuals.Keywords: Bisoprolol, Triamterene, Liquid chromatography-mass spectrometry/mass spectrometry, Method validation, Human plasma,Pharmacokinetics.

show abstract

Liquid chromatography tandem mass spectrometry method for determination of bisoprolol in human plasma using d5‐bisoprolol as the internal standard

Cited by 9 publications

References 15 publications

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

A HPLC-MS/MS Method Development and Validation for the Simultaneous Determination of Bisoprolol and Enalapril in the Present of Enalaprilat in Human Plasma

Sensitive LC-MS/MS Method for the Simultaneous Determination of Bisoprolol and Triamterene in human plasma

Contact Info

Product

Resources

About