Preparation and characterization of ciprofloxacin‐loaded alginate/chitosan sponge as a wound dressing material

Abstract

ABSTRACT:The aim of this study is preparation and characterization of alginate/chitosan sponges including a model antibiotic (i.e., ciprofloxacin) to use in wound and/or burn treatment. Sponges were prepared firstly by the gelation of sodium alginate followed by lyophilization, crosslinking with calcium chloride, and finally coating with chitosan. Sponges were characterized with respect to morphology, water uptake, in vitro drug release behavior, and antimicrobial activity. Investigated and evaluated parameters in all of these studies were selected as the concentration of calcium chloride, alginate viscosity, drug content, and molecular weight of chitosan. Drug release and water uptake were found to be greatly influenced by these parameters. Water uptake and drug release rate were decreased by increasing the crosslinking density, chitosan molecular weight, and alginate viscosity. In the antimicrobial tests, it was obtained that the antimicrobial activity is directly proportional with the release rates and water uptake. Morphological studies showed a highly porous structure with interconnected pores.

Alginate–lavender nanofibers with antibacterial and anti-inflammatory activity to effectively promote burn healing

“…15 It has been also demonstrated that films of this polysaccharide and chitosan in combination with laser therapy help the epithelization and vascularization of dermal burns. 16 Furthermore, since burn wounds are highly exposed to the risk of microbial colonization that possibly determines local and systemic infections and delays the healing process, 17,18 alginate dressings containing silver 19,20 and other antimicrobial compounds 21 have been developed. Despite the benefits offered by Ca-alginate based biomedical devices, studies in literature have pointed out that the release of Ca 2+ ions from the dressing to the wound site stimulates the production of pro-inflammatory cytokines, such as Interleukin-6 (IL-6), Interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and the chemotactic cytokine IL-8.…”

Composite poly(vinyl alcohol)/poly(vinyl acetate) electrospun nanofibrous mats as a novel wound dressing matrix for controlled release of drugs

“…4,5 Furthermore, a proper wound dressing needs to have wound healing properties, including the essential swelling capability for absorbing excess exudates and oxygen permeability for respiring. 5,6 Modern dressings as vehicles for delivering therapeutic agents to wound sites have been applied in the form of hydrogels, 2 films, 7 sponges, 8 foams, 9 and recently in the form of nanofibers. 6,10 However, little research has been published on the controlled delivery of drugs from polymeric dressing, especially in the form of nanofibers.…”

“…As a result, the high degree of swelling along with the dissolution of the PVA nanofiber mats, which is attributed to its poor stability in aqueous mediums, causes a quick burst release, as reported previously. 8,20,18 According to these profiles, increasing the drug content in PVA nanofiber mats from 5% to 10% w/w lowered initial drug release and improved the control of the release profile. According to Figure 4A, at the initial experiment stages, a higher drug content (10% w/w) in PVA nanofiber mats results in a lower degree of swelling.…”

Recent Trends in Alginate, Chitosan and Alginate-Chitosan Antimicrobial Systems

“…Alginate-chitosan sponge represent a tridimensional porous scaffold, prepared by freeze-drying technique of mixture [83] or consecutively deposited one above the other layers of alginate and chitosan [84][85][86]. Pore sizes range from <100 μm [85,86], 200-400 μm [83] and approximately 500 μm [84], as a function of various factors such as cross-linking time with Ca 2+ ions -with its increasing pore sizes being reduced [83] (Figure 8).…”