10⁻⁷ m 17β‐oestradiol enhances odonto/osteogenic potency of human dental pulp stem cells by activation of the NF‐κB pathway

Abstract

ObjectivesOestrogen has been proven to significantly enhance osteogenic potency, while oestrogen deficiency usually leads to impaired osteogenic differentiation of mesenchymal stem cells. However, little is known concerning direct effects of oestrogen on differentiation of human dental pulp stem cells (DPSCs).Materials and methodsIn this study, human DPSCs were isolated and treated with 10−7 m 17β-oestradiol (E2). Alkaline phosphatase (ALP) assay and alizarin red staining were performed.ResultsAlkaline phosphatase and alizarin red showed that E2 treatment significantly enhanced ALP activity and mineralization ability of DPSCs, but had no effect on cell proliferation. Real-time RT-PCR and western blot assay demonstrated that odonto/osteogenic markers (ALP, RUNX2/RUNX2, OSX/OSX, OCN/OCN and DSPP/DSP) were significantly upregulated in the cells after E2 treatment. Moreover, phosphorylation of cytoplasmic IκBα/P65 and expression of nuclear P65 were enhanced in a time-dependent manner following E2 treatment, suggesting activation of NF-κB signaling. Conversely, inhibition of the NF-κB pathway suppressed E2-mediated upregulation of odonto/osteogenic markers, indicating that the NF-κB pathway was pivotal for E2-mediated differentiation.ConclusionThese findings provide evidence that 10−7 m 17β-oestradiol promoted odonto/osteogenic differentiation of human DPSCs via activation of the NF-κB signaling pathway.

Oestrogen retains human periodontal ligament stem cells stemness in long‐term culture

“…E2 promotes hematopoietic stem cells (HSCs) self‐renewal and erythropoiesis in the spleen, inhibits BMSCs apoptotic rate, and increase colony‐forming via modulating Bcl‐2 and Bcl‐xL proteins . Furthermore, E2 can enhance proliferation and odonto/osteogenic differentiation of human dental pulp stem cells and stem cells from apical papilla . We found that short‐term E2 treatment significantly improved the ratio of (G2/M+S) phases and the expression of cyclin D1 (Figures and ).…”

“…Oestrogen (E2) is a hormone that plays a key role in body metabolism, affecting biological processes that include bone formation, angiogenesis and autoimmunity . Recently, studies reported that exogenous E2 enhanced the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), dental pulp stem cells and stem cells from apical papilla . It suggests that E2 might have the potential to enhance the proliferation and osteogenic differentiation of hPDLSCs during in vitro culture.…”

Human Dental Pulp Stem Cells via the NF-κB Pathway

“…BMS-345541 (a highly selective inhibitor of NF- κ B) was used to block the phosphorylation of IκBα and p65 [20,21,22], following which ALP activity and expression levels of several gene markers of differentiation were assessed. As expected, BMS-345541 significantly inhibited the increase of ALP activity and the mRNA expression of ALP, OCN, DSPP, Col I and RUNX2 induced by ZBTB20 (Fig.…”

“…Lentivirus production and infection were performed as previously described [22]. HDPSCs were infected with recombinant lentivirus-transducing units in the presence of 8 µg/mL polybrene.…”

Dental Pulp Stem Cell Niche

“…TNF-α (an activator of NF-κB pathway) enhances the odontoblastic phenotype of DPSCs [104]. 17β-estradiol promotes the osteo/odontogenic potency of human DPSCs by activation of NF-κB signaling pathway [105]. Moreover, DPSCs from the injured pulp present a lower proliferative capacity, upregulated osteogenic potential and weakened odontogenic capacity than healthy DPSCs and this process is also associated with NF-κB signaling pathway [106].…”