[10] Gangliosides: Structure, isolation, and analysis

Ledeen, Robert W.; Yu, Robert K.

doi:10.1016/0076-6879(82)83012-7

Cited by 655 publications

(311 citation statements)

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“…8 The extract was clarified by centrifugation and dried under a stream of nitrogen. For purification of the gangliosides 9 the dried total lipid extract was partitioned in di-isopropylether, 1-butanol, 50 mmol/1 aqueous sodium chloride (in the ratio 6/4/5 by volume: 3 vol/ml plasma, 30 vol/g tissue).…”

Section: Isolation and Purification Of Gangliosidesmentioning

confidence: 99%

Detection of a Tumour-Associated Ganglioside in Plasma of Patients With Neuroblastoma

Ladisch¹,

Wu²

1985

The Lancet

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SummaryAn abnormal circulating ganglioside was found in patients with neuroblastoma. This ganglioside appeared as a single band by resorcinol-HCl staining of thin-layer chromatograms of purified total gangliosides isolated from as little as 1 ml of patient plasma. It is a major ganglioside of neuroblastoma tumour tissue and was present (250-1500 pmol lipid-bound sialic acid/ml) in the plasma of five patients with widespread neuroblastoma. In contrast, the ganglioside was not detected (<50 pmol/ml) in plasma samples of six patients in complete remission, nor in plasma samples of seventeen healthy children and adults. Measurement of this circulating tumourassociated ganglioside should be clinically useful in neuroblastoma, offering a new approach to the detection of tumour and the evaluation of therapy.

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Section: Isolation and Purification Of Gangliosidesmentioning

confidence: 99%

Detection of a Tumour-Associated Ganglioside in Plasma of Patients With Neuroblastoma

Ladisch¹,

Wu²

1985

The Lancet

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“…This result is promising, as the previously developed TLC separation methods can be coupled to MALDI-FTMS. G angliosides constitute a class of glycolipids that occur in the outer leaflet of the plasma membrane in all eukaryotic cells [1][2][3]. Gangliosides bear one or more N-acyl neuraminic acid (sialic acid) residues as branches on their carbohydrate moiety, an oligosaccharide with a linear backbone that is linked to a ceramide which may have heterogeneity in both its long-chain base and N-linked fatty acyl group [4,5].…”

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confidence: 99%

“…Gangliosides play important roles at the cell surface, serving as antigens and/or receptors, e.g., for adhesion [6,7], attachment of extracellular matrix components, viruses, bacteria and toxins [8,9], and as an important means for cell-cell communication [3,10]. The variations in their structures are especially important during nervous system development and carcinogenesis [2,[11][12][13]. Like most glycoconjugates, gangliosides usually occur as mixtures of structural variants whose specific biological activity at any given time may be based on minor, transient components in the mixture.…”

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confidence: 99%

“…Like most glycoconjugates, gangliosides usually occur as mixtures of structural variants whose specific biological activity at any given time may be based on minor, transient components in the mixture. It is often not possible to obtain enough pure material for NMR structural determinations of the critical epitopes, and even when larger amounts of sample may be available, the progress of the isolation must be monitored by techniques with high specificity and sensitivity [2,9,14]. Due to its high sensitivity and capacity for dealing with complex mixtures, mass spectrometry often plays a critical role in the detection and structural determination of gangliosides.…”

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High pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for minimization of ganglioside fragmentation

O’Connor

Mirgorodskaya

Costello

2002

J. Am. Soc. Mass Spectrom.

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Transiently elevating pressure in a matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) source into the 1-10 mbar range during ionization decreases the metastable fragmentation of gangliosides. This allows detection of the molecular ion species without loss of the highly labile sialic acid residues. In these experiments, gangliosides with up to five sialic acids were ionized by MALDI and detected with the FTMS. In each case, when the high pressure collisional cooling was used, the singly charged molecular ion was the base peak in the spectra, both in the positive and negative ion modes, and minimal metastable fragmentation was observed. This result is promising, as the previously developed TLC separation methods can be coupled to MALDI-FTMS. (J Am Soc Mass Spectrom 2002, 13, 402-407)

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“…Gangliosides are a family of sialylated glycosphingolipids located in higher density in nervous system, especially in axons of neuron (Ledeen & Yu, 1982; Schuster & Haller, 1990). It consists of several subtypes depending on the number and position of sialic acids, the number of glucose molecules, and their synthetic pathways, for example, GM1, GM2, GM3, GD1a, GD1b, GT1b, and GQ1b and so on (Asthana et al., 2016; Yuki, 2012).…”

Section: Introductionmentioning

confidence: 99%

Association of anti‐gangliosides antibodies and anti‐CMV antibodies in Guillain–Barré syndrome

et al. 2017

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IntroductionNumerous types of infection were closely related to GBS, mainly including Campylobacter jejuni, Cytomegalovirus, which may lead to the production of anti‐gangliosides antibodies (AGA). Currently, although there are increased studies on the AGA and a few studies of anti‐CMV antibodies in GBS, the association between them remains poorly documented. Therefore, our research aims to analyze the correlation of anti‐CMV antibodies and AGA in GBS.MethodsA total of 29 patients with GBS were enrolled in this study. The CMV antibodies were tested by the electrochemiluminescence immunoassay “ECLIA” (Roche Diagnostics GmbH). The serum gangliosides were determined by The EUROLINE test kit.ResultsOf the 29 patients with GBS, 9 (31%) were AGA‐seropositive, in which 22 were CMV‐IgG positive in CSF at the same time, but all 29 samples were CMV‐IgM negative in both serum and CSF. In the AGA‐positive group, the rate of both serum and CSF positive was 87.5% (7/8), higher than 50% (7/14) of the negative group, although no statistical significance was found. In addition, we found that there was a trend of higher ratio of men, a younger age onset, less frequent preceding infection, a higher level of CSF proteins, and less frequent cranial nerve deficits, although the data did not reach a statistical significance.ConclusionIn spite of no statistical significance association was found between serum AGA and CMV‐IgG in serum and CSF. However, we found that there was a trend of high positive rate of both serum and CSF‐CMV‐IgG in AGA‐positive than the negative group. So we should further expand the sample size to analyze the association between AGA and CMV or other neurotropic virus antibodies in various diseases, to observe whether they could be serological marker of these diseases (especially GBS) or the underlying pathogenesis.

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[10] Gangliosides: Structure, isolation, and analysis

Cited by 655 publications

References 168 publications

Detection of a Tumour-Associated Ganglioside in Plasma of Patients With Neuroblastoma

Detection of a Tumour-Associated Ganglioside in Plasma of Patients With Neuroblastoma

High pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for minimization of ganglioside fragmentation

Association of anti‐gangliosides antibodies and anti‐CMV antibodies in Guillain–Barré syndrome

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