10.5937/racter6-4032 = Xenobiotics-induced hepatotoxicity and an influence of HLA typisation

Žunić, Miodrag Vojislav; Zunic, D; Žunić, Marko; Jesic, Rada

doi:10.5937/racter6-4032

Racion ter

2014

DOI: 10.5937/racter6-4032

|View full text |Cite

10.5937/racter6-4032 = Xenobiotics-induced hepatotoxicity and an influence of HLA typisation

Miodrag Vojislav Žunić¹,

D Zunic²,

Marko Žunić³

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2022

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liposuction is the second most popular aesthetic procedure performed in the United States 1 . It is commonly assisted by mechanical suction, also known as suction-assisted liposuction, power-assisted liposuction, ultrasound, or laser energy 2–4 . Laser-assisted liposuction (LAL) is a cosmetic, minimally invasive surgical procedure that allows rapid, comfortable postoperative recovery and quick return to daily activities 5 .…”

mentioning

confidence: 99%

High-Quality Lipoaspirate Following 1470-nm Radial Emitting Laser-Assisted Liposuction

et al. 2022

View full text Add to dashboard Cite

Background: Laser-assisted liposuction (LAL) has been used to maximize viable adipocyte yields in lipoaspirates, although optimizing tissue processing methods is still a challenge. A high-quality lipoaspirate has been a key factor for extended graft longevity. Objective: To assess the viability and potency of stromal vascular fraction (SVF) cells and adipose-derived stem cells (ASCs) in fat samples from lipoaspirates harvested with a novel 1470-nm diode, radial emitting LAL platform. Two processing methods, enzymatic and nonenzymatic, were compared. Methods: Laser-assisted liposuction lipoaspirates harvested from 10 subjects were examined for cell viability after processing by enzymatic or nonenzymatic methods. Isolated SVF cells were cultured with an ASC-permissive medium to assess their viability and proliferation capacity by cell proliferation assay. Flow cytometric analysis with ASC-specific markers, gene expression levels, and immunofluorescence for ASC transcription factors were also conducted. Results: Lipoaspirates showed high SVF cell viability of 97% ± 0.02% and 98% ± 0.01%, averaged SVF cell count of 8.7 Â 10 6 ± 3.9 Â 10 6 and 9.4 Â 10 6 ± 4.2 Â 10 6 cells per mL, and averaged ASC count of 1 Â 10 6 ± 2.2 Â 10 5 and 1.2 Â 10 6 ± 5 Â 10 5 cells per mL in nonenzymatic and enzymatic methods, respectively. The ASC-specific markers, gene expression levels, and immunofluorescence for ASC transcription factors confirmed the adipose origin of the cells. Conclusions: The laser lipoaspirates provide a high yield of viable and potent SVF cells and ASCs through both nonenzymatic and enzymatic processes. Improved purity of the harvested lipoaspirate and high ASC content are expected to result in extended graft longevity. Furthermore, eliminating enzymatic digestion may provide advantages, such as reducing process time, cost, and regulatory constraints.

show abstract

mentioning

confidence: 99%

High-Quality Lipoaspirate Following 1470-nm Radial Emitting Laser-Assisted Liposuction

et al. 2022

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

10.5937/racter6-4032 = Xenobiotics-induced hepatotoxicity and an influence of HLA typisation

Cited by 1 publication

References 29 publications

High-Quality Lipoaspirate Following 1470-nm Radial Emitting Laser-Assisted Liposuction

High-Quality Lipoaspirate Following 1470-nm Radial Emitting Laser-Assisted Liposuction

Contact Info

Product

Resources

About