1. The Navy’s R&amp;D Program

Harwood, Julius J.

doi:10.1007/bf03378009

Cited by 2 publications

(3 citation statements)

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“…After 15 min incubation at 3 7 T , lysate was obtained by microfugation (1 1 OOO g, 5 min). The assay monitors the conversion of NADP to NADPH as a change in absorbance at 340 nm, with isocitrate as the substrate (Harwood et al 1990). Cell lysate or supernatant fluid (420 pl) is mixed with 100 mmol/l potassium phosphate buffer (pH 8.0; 500 PI), 100 mmol/l isocitrate (pH adjusted to 7.0 with NaOH; 10 pl), 250 mmol/l MgCI, (20 pl) and 8 mmol/l NADP in phosphate buffer (50 pl).…”

Section: Test For C E L L L Y S I Smentioning

confidence: 99%

Protein export during growth of Bacillus subtilis: the effect of extracellular protease deficiency

Coxon

Harwood

Archibald

1991

Lett Appl Microbiol

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Exponential phase cultures of a protease‐deficient and a protease‐proficient strain of Bacillus subtilis, growing in minimal medium with a glucose carbon source, were labelled with 14C‐phenylalanine. Subsequent export of labelled protein was detected in trichloroacetic acid‐precipitated supernatant fluid samples. During exponential growth the strains exhibited substantially similar protein export kinetics. However, as confirmed by the presence of a cytoplasmic enzyme in the culture supernatant fluid, protease deficiency was associated with an increased tendency of cells to lyse as they approached stationary phase.

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Section: Test For C E L L L Y S I Smentioning

confidence: 99%

Protein export during growth of Bacillus subtilis: the effect of extracellular protease deficiency

Coxon

Harwood

Archibald

1991

Lett Appl Microbiol

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“…The timber is suitable for building poles, pilings, posts, heavy construction and for pulp (CLARK et al, 1999;HARWOOD, 2001;MARCAR and CRAW-FORD, 2004). It has a narrow natural distribution in southern wheatbelt and subcoastal areas of Western Australia at an elevation of 50-300 m and occupies a wide variety of habitats, from riparian areas, including swamps and salt lakes, to xeric landscapes (CHIPPEN-DALE, 1973;BROOKER and KLEINIG, 1990).…”

Section: Eucalyptus Occidentalis Endlmentioning

confidence: 99%

“…This species has moderate to high tolerance to salt and waterlogging (PEPPER and CRAIG, 1986;MARCAR et al, 1995MARCAR et al, , 2003BENYON et al, 1999) and there is interest in planting E. occidentalis for rehabilitation and reclamation of saline areas especially for agricultural land suffering from waterlogging and increasing dryland salinity. Eucalyptus occidentalis was selected as one of six species for tree improvement by the Australian Low Rainfall Tree Improvement Group (HARWOOD et al, 2001 E. occidentalis would facilitate its deployment as a suitable plant for management of saline agricultural land in southern Australia.…”

Section: Eucalyptus Occidentalis Endlmentioning

confidence: 99%

Effect of genetic relatedness among parents on gain in salt tolerance in progeny of crosses ofEucalyptus occidentalis

Hendrati

Byrne

Barbour³

2011

Silvae Genetica

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Genetic diversity of a Eucalyptus occidentalis breeding population screened for salt and waterlogging tolerance was examined using eight microsatellite loci. Mating using an immature style 'one stop pollination' method between parents was carried out to produce progeny for testing under 500 mM salt waterlogging. The effect of parental genetic distance on seed production and early seedling survival was examined and inheritance of salt/or waterlogging tolerance was assessed by testing performance of progeny in comparison to parents. Diversity was moderate among the nine provenances and the families, and most of the diversity was distributed within rather than between families. Genetic relationships showed no structure in relation to provenance indicating any adaptation to saline environments has not affected genetic similarity. Breeding for salt and water tolerance may be achieved without decline in genetic diversity. There was a significant correlation between capsule production and parental genetic distance and a positive trend between increasing parental genetic distance and increasing number of germinated seeds/capsule, and seedling survival at 2 weeks and 9 months. These trends indicate expression of inbreeding depression in crosses between genetically similar parents. Heritability values, under 500 mM saltwaterlogging treatment, indicated that height had moderate heritability (h 2 = 0.5). Crosses with the widest parental genetic distance produced progeny with considerable height increase above parents and this trend was evident even with moderate genetic distance indicating crossing at this level of differentiation may achieve optimum breeding gain.

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1. The Navy’s R&D Program

Cited by 2 publications

References 0 publications

Protein export during growth of Bacillus subtilis: the effect of extracellular protease deficiency

Protein export during growth of Bacillus subtilis: the effect of extracellular protease deficiency

Effect of genetic relatedness among parents on gain in salt tolerance in progeny of crosses ofEucalyptus occidentalis

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