[1] Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids

Omaye, Stanley T.; Turnbull, John D.; Sauberlich, Howerde E.

doi:10.1016/0076-6879(79)62181-x

Cited by 1,454 publications

(722 citation statements)

References 13 publications

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“…The analysis of each i.e. catalase (method of Maehly and Chance) [5], glutathione (DTNB method) [6], albumin (BCG method) [7], ascorbic acid (2, 6-dichlorophenolindophenol method) [8] and malondialdehyde (method of Buege et al) [9] was done using standard methods. The statistical analysis was performed using Software Package for Social Sciences (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Status and Oxidative Stress in the Circulation of Younger and Elderly Human Subjects

et al. 2012

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Extensive research has demonstrated the protective properties of antioxidants, which scavenge reactive oxygen species and their precursors, as well as up-regulate enzymes involved in the repair of cellular damage. Several case-control studies have showed higher blood levels of antioxidants and decreased oxidative stress in younger individuals when compared with older ones. Cell damage caused by free radicals appears to be a major contributor in aging and degenerative diseases of aging such as cancer, cardiovascular disease, cataracts, compromised immune system, rheumatoid arthritis and brain dysfunction. The objective of this study was to determine the variation of Circulating levels of selected antioxidants (enzymic and non enzymic) and oxidative stress marker in younger and older humans. The results showed that a majority of the younger age group participants showed a significant increase in enzymic and nonenzymic antioxidant status and a decrease in oxidative stress when compared with the older age group.

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Section: Methodsmentioning

confidence: 99%

Antioxidant Status and Oxidative Stress in the Circulation of Younger and Elderly Human Subjects

et al. 2012

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“…22 Tissue ascorbate levels were estimated using the 2,4-dinitrophenylhydrazine method. 23 Catalase activity was assayed by the method of Aebi and expressed as mmol of H 2 O 2 consumed per min per mg protein (MEC ¼ 44.1 mM -1 cm -1 ). 24 Superoxide dismutase activity was measured by monitoring the inhibition of quercetin autoxidation.…”

Section: Methodsmentioning

confidence: 99%

Evidence of oxidative stress and mitochondrial dysfunctions in the testis of prepubertal diabetic rats

Chandrashekar

Muralidhara

2009

Int J Impot Res

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Earlier, we have shown the occurrence of oxidative impairments and their progression in the testis of diabetic adult rats. This study investigated the vulnerability of immature testis to oxidative stress and mitochondrial dysfunctions in a prepubertal (PP) diabetic rat model. PP male rats (4/6-weekold) rendered diabetic by an acute dose of streptozotocin were monitored for induction of oxidative stress in testis cytosol/mitochondria. Diabetic rats of both age groups showed severe hyperglycemia, testicular atrophy and marked oxidative damage as evidenced by enhanced generation of reactive oxygen species, hydroperoxide and malondialdehyde levels (4 week46 week). Mitochondrial dysfunctions manifested as reduction in the activities of aldehyde dehydrogenase, tricarboxylic acid cycle enzymes, enhanced activities of oxidative phosphorylation enzymes, perturbations in calcium homeostasis and membrane potential. These evidences suggest that an immature testis is vulnerable to oxidative stress under diabetes, which may play a significant role in the development of testicular degeneration, leading to impaired fertility in adulthood.

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“…Assay of vitamin E was performed in plasma (Baker & Frank, 1968). Glutathione (GSH) and vitamin C (ascorbic acid), a water soluble antioxidant were estimated in the whole blood as described in Moron et al (1979) and Omaye et al (1979).…”

Section: Groupingmentioning

confidence: 99%

Protective role of vitamin E on the oxidative stress in Hansen's disease (Leprosy) patients

et al. 2005

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Background: A constellation of reactive oxygen species (ROS) capable of damaging cellular constituents generated in excess during the chronic, inflammatory, neurodegenerative disease process of leprosy. The consequences of this leads to enhanced oxidative stress and lower antioxidant status. Enzymatic antioxidants provide first line defense against ROS. We have measured the levels of oxidative stress indices like lipid peroxidation (LPO), protein carbonyls together with enzymatic antioxidants in the blood samples of control and leprosy patients. Nutritional rehabilitation by way of exogenous supplementation of functionally efficient antioxidants like vitamin E reactivates the enzymatic antioxidant system and guards against the insult caused by ROS during the pathogenesis of the disease and antileprosy chemotherapy. Design: Untreated leprosy patients were selected on the basis of clinical examination and skin smear. All diagnosed untreated leprosy patients received multi drug therapy (MDT) consisting of rifampicin, dapsone and clofazimine as recommended by World Health Organization. A small number of untreated cases were selected for co-supplementation of vitamin E along with MDT. Oxidative stress indices, enzymatic and nonenzymatic antioxidant status were assayed in untreated, MDT treated and those supplemented vitamin E along with MDT. Statistical methods: We have compared the significance in the mean7s.d. values of the oxidative stress indices and the levels of antioxidants using one way analysis of variance (ANOVA) between control, untreated, MDT treated and those supplemented vitamin E with MDT and the results were significant at Po0.05. Statistical analysis of the results suggests that oral administration of vitamin E lowers oxidative stress and augments antioxidant status in affected individuals. Results: Enhanced oxidative stress as evidenced by increased LPO and protein carbonyl in leprosy cases lowers the antioxidant status. Treatment with MDT has a limited impact on increased oxidative stress and decreased antioxidant status. Coadministration of vitamin E along with MDT decreases oxidative stress and activate the antioxidant status. Discussion: The excess production of ROS as seen in leprosy cases could lead to degeneration of tissues and derangement of internal organs. The possible reason for the decreased antioxidant status in leprosy cases may be increased production of ROS, deranged liver function, and the free radical producing ability of drugs used in MDT of leprosy. Intervention with antioxidant supplementation like vitamin E prevents oxidative stress mediated through ROS and activates the net antioxidant status during the chronic course of the disease and antileprosy chemotherapy.

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[1] Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids

Cited by 1,454 publications

References 13 publications

Antioxidant Status and Oxidative Stress in the Circulation of Younger and Elderly Human Subjects

Antioxidant Status and Oxidative Stress in the Circulation of Younger and Elderly Human Subjects

Evidence of oxidative stress and mitochondrial dysfunctions in the testis of prepubertal diabetic rats

Protective role of vitamin E on the oxidative stress in Hansen's disease (Leprosy) patients

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