[1] Immobilized active coenzymes

Månsson, Mats-Olle; Mosbach, Klaus

doi:10.1016/s0076-6879(87)36003-3

Cited by 15 publications

(11 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mixture in the upper compartment was filtrated using quartz glass wool (A grade), and a 200 μL sample was taken for the gel filtration chromatography. The concentration of the bound nucleotides was determined by using the UV spectroscopic method as described elsewhere (1, 9, 12, 14), assuming ϵ = 15 000 1/(M·cm) at 260 nm.…”

Section: Methodsmentioning

confidence: 99%

“…The biosyntheses that require ATP need a continuous regeneration of ATP to make feasible production economic. Of the methods available for the regeneration of ATP, the enzymatic method has been chosen to be the most suitable route for industrial applications (1–4).…”

Section: Introductionmentioning

confidence: 99%

“…Several water‐soluble polymers have been used for the enlargement of adenine nucleotides. Such polymers are dextran (9–12), polyethyleneimine (1), acrylic copolymer (13), acrylamide (14, 15), and poly(ethylene glycol) (2). Although these polymer‐bound adenine nucleotides are useful for specific applications, the synthetic procedures are often complicated.…”

Section: Introductionmentioning

confidence: 99%

“…These include high branching density, regular structures with practically no defects, and a large number of terminal functional groups and are available in different generations with different molecular masses (16). In addition, it was reported that the coenzymatic activity of an adenine nucleotide bound on a polymer chain depended on its position in the polymer (near or far from the polymer backbone), the polymer hydrophilic/hydrophobic character, and the polymer size (1). In the case of the PAMAM dendrimers the adenine nucleotides could be coupled with the terminal functional groups directly with allowing adequately far distance between the dendrimer central core and the adenine moieties.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Synthesis of New Polymer‐Bound Adenine Nucleotides Using Starburst PAMAM Dendrimers

Abdelmoez

Yasuda

Ogino

et al. 2002

Biotechnology Progress

View full text Add to dashboard Cite

Two types of new polymer-bound adenine nucleotides were synthesized by coupling adenine nucleotides (ATP and ADP) with starburst polyamidoamine (PAMAM) dendrimers. The first type was obtained by coupling native adenine nucleotides directly with a carboxy-terminated PAMAM dendrimer. In the second type, the nucleotides were modified by introducing a spacer arm containing a carboxylic end group (N(6)-R-ATP and N(6)-R-ADP) and coupled with an amine-terminated PAMAM dendrimer. Both types of the dendrimers were coupled with native or the modified nucleotides using the well-known carbodiimide activation technique. The optimum coupling pH and temperature were 4 and 30 degrees C, respectively, for preparing the carboxy-terminated PAMAM-bound ATP or ADP, and were 9 and 50 degrees C, respectively, for preparing the amine-terminated PAMAM-bound N(6)-R-ATP or N(6)-R-ADP. The ATP or ADP contents in the synthesized polymers were found to be 4 mol of ATP or of ADP/mol of carboxy-terminated PAMAM-bound ATP or ADP and 25 mol of ATP or of ADP/mol of amine-terminated PAMAM-bound N(6)-R-ATP or N(6)-R-ADP. The coenzymatic activities relative to the native ATP of the carboxy-terminated PAMAM-bound ATP against glucokinase and hexokinase were 16 and 7%, respectively, and those of the amine-terminated PAMAM-bound N(6)-R-ATP 2 and 1%, respectively. The coenzymatic activities relative to the native ADP of the carboxy-terminated PAMAM-bound ADP and the amine-terminated PAMAM-bound N(6)-R-ADP against acetate kinase were 24 and 3.5%, respectively.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Synthesis of New Polymer‐Bound Adenine Nucleotides Using Starburst PAMAM Dendrimers

Abdelmoez

Yasuda

Ogino

et al. 2002

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…This was suggested from the analysis of enzyme-coenzyme-substrate complexes [84]. Tables with chemically-modified coenzymes are given in [85,86] for both NAD + and ATP. One approach to obtaining this type of compound was to prepare water-soluble (because they show less diffusional hindrance) macromolecular derivatives of the cofactors and to attach them to high-molecular weight soluble polymers through a suitable linkage in order to assure coenzymic activity and stability of the cofactor-polymer bond.…”

Section: Nad(p)(h) Derivativesmentioning

confidence: 99%

Redox Enzymes Used in Chiral Syntheses Coupled to Coenzyme Regeneration

Leonida¹

2001

CMC

View full text Add to dashboard Cite

Due to their ability to perform reactions in a stereo- and regiospecific manner, while functioning under mild conditions of temperature and pH, enzymes are increasingly used for chiral synthesis in the pharmaceutical industry, in medicinal chemistry, agriculture, cosmetic and food industry. The oxidoreductases as catalysts require a coenzyme (cofactor) which functions as an electron carrier. If used in stoichiometric amounts the cost of coenzymes makes synthetic applications of redox enzymes prohibitively expensive for industrial applications. The present review updates previous discussions about the objectives of cofactor regeneration as a requirement for the applicability of enzymatic redox reactions, and about the strategies customarily used to this end. Different types of chiral syntheses coupled successfully to coenzyme regeneration (amino acid synthesis, lactone synthesis, synthetic reactions involving alcohols, hydroxy acid and steroid synthesis, deracemization) are then discussed. New catalysts, cross-linked enzyme crystals, prepared from redox enzyme are presented in a small paragraph together with some of their applications. Special attention is given to whole cells used in this type of synthesis and their advantages and disadvantages are presented in one paragraph. Finally, different reactors, within which were conducted chiral syntheses catalyzed by oxireductases and coupled to cofactor regeneration, are briefly discussed.

show abstract