1-Hydroxypyrene as a biomarker for assessing the effects of exposure to polycyclic aromatic hydrocarbons on semen quality and sperm DNA integrity

Jeng, Hueiwang Anna; Pan, Chih-Hong; Chao, Mu‐Rong

doi:10.1080/03601234.2012.716741

Cited by 27 publications

(14 citation statements)

References 43 publications

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“…As previously reported, DEPs disrupt male reproductive function resulting in reduction of daily sperm production and motility, increased morphological sperm abnormalities, ultrastructural changes in Leydig cells, elevation of serum testosterone, and reduction of LH level [Watanabe and Oonuki, ; Yoshida et al, ; Tsukue et al, ; Yoshida and Takeda, ; Izawa et al, ; Li et al, , 2012; Ramdhan et al, ]. Human exposure to PAHs causes modification to male sperm quality, including morphology, concentration, and vitality, as well as causing DNA damage in sperm, thus affecting male reproductive function [Gaspari et al, ; Meeker et al, ; Han et al, ; Jeng et al, ]. Some PAHs, such as benzo( a )pyrene, fluoranthene, or benzo( ghi )perylene (chemically reactive and nonvolatile), are emitted at greater levels in biodiesel than diesel exhaust [Kado et al, ] and are well‐known endocrine disrupters [Raychoudhury and Kubinski, ].…”

Section: Discussionmentioning

confidence: 88%

“…Most of the particles from these engine exhausts are of nanoscale and potentially have hundreds of chemicals absorbed onto their surfaces, including known and suspected mutagens and carcinogens, for example, polycyclic aromatic hydrocarbons (PAH) and nitrated PAH (nPAH) [Mermelstein et al, 1981;Ohe, 1984;Rivedal et al, 2003;B€ unger et al, 2012]. Recently, it has been shown that human exposure to PAHs causes alterations in male sperm quality, including morphology, concentration, and vitality, as well as DNA damage, thus affecting male reproductive function [Gaspari et al, 2003;Meeker et al, 2007;Han et al, 2011;Jeng et al, 2013].…”

Section: Introductionmentioning

confidence: 99%

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Abnormalities in the male reproductive system after exposure to diesel and biodiesel blend

Kisin

Yanamala

Farcas

et al. 2014

Environ and Mol Mutagen

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Altering the fuel source from petroleum-based ultra-low sulfur diesel to biodiesel and its blends is considered by many to be a sustainable choice for controlling exposures to particulate material. As the exhaust of biodiesel/diesel blends is composed of a combination of combustion products of polycyclic aromatic hydrocarbons and fatty acid methyl esters, we hypothesize that 50% biodiesel/diesel blend (BD50) exposure could induce harmful outcomes because of its ability to trigger oxidative damage. Here, adverse effects were compared in murine male reproductive organs after pharyngeal aspiration with particles generated by engine fueled with BD50 or neat petroleum diesel (D100). When compared with D100, exposure to BD50 significantly altered sperm integrity, including concentration, motility, and morphological abnormalities, as well as increasing testosterone levels in testes during the time course postexposure. Serum level of luteinizing hormone was significantly depleted only after BD50 exposure. Moreover, we observed that exposure to BD50 significantly increased sperm DNA fragmentation and the upregulation of inflammatory cytokines in the serum and testes on Day 7 postexposure when compared with D100. Histological evaluation of testes sections from BD50 exposure indicated more noticeable interstitial edema, degenerating spermatocytes, and dystrophic seminiferous tubules with arrested spermatogenesis. Significant differences in the level of oxidative stress assessed by accumulation of lipid peroxidation products and depletion of glutathione were detected on exposure to respirable BD50 and D100. Taken together, these results indicate that exposure of mice to inhalable BD50 caused more pronounced adverse effects on male reproductive function than diesel.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Abnormalities in the male reproductive system after exposure to diesel and biodiesel blend

Kisin

Yanamala

Farcas

et al. 2014

Environ and Mol Mutagen

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“…Urinary 1-OHP has served as a reliable biomarker for PAH exposure and its biological metabolism in the entire biological system. However, a recent study has suggested that urinary 1-OHP may not be sufficient to assess the effect of PAHs on sperm DNA integrity and associated reproductive capacity [55]. …”

Section: Discussionmentioning

confidence: 99%

Sperm DNA oxidative damage and DNA adducts

Jeng

Pan

Chao

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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The objective of this study was to investigate DNA damage and adducts in sperm from coke oven workers who have been exposed to polycyclic aromatic hydrocarbons. A longitudinal study was conducted with repeated measurements during spermatogenesis. Coke-oven workers (n=112) from a coke-oven plant served the PAH-exposed group, while administrators and security personnel (n=67) served the control. Routine semen parameters (concentration, motility, vitality, and morphology) were analyzed simultaneously; the assessment of sperm DNA integrity endpoints included DNA fragmentation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGuo). The degree of sperm DNA fragmentation was measured using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and sperm chromatin structure assay (SCSA). The PAH-exposed group had a significant increase in bulky DNA adducts and 8-oxo-dGuo compared to the control subjects (Ps = 0.002 and 0.045, respectively). Coke oven workers' percentages of DNA fragmentation and denaturation from the PAH-exposed group were not significantly different from those of the control subjects (Ps = 0.232 and 0.245, respectively). Routine semen parameters and DNA integrity endpoints were not correlated. Concentrations of 8-oxo-dGuo were positively correlated with percentages of DNA fragmentation measured by both TUNEL and SCSA (Ps = 0.045 and 0.034, respectively). However, the concentrations of 8-oxo-dGuo and percentages of DNA fragmentation did not correlate with concentrations of bulky DNA adducts. In summary, coke oven workers with chronic exposure to PAHs experienced decreased sperm DNA integrity. Oxidative stress could contribute to the degree of DNA fragmentation. Bulky DNA adducts may be independent of the formation of DNA fragmentation and oxidative adducts in sperm. Monitoring sperm DNA integrity is recommended as a part of the process of assessing the impact of occupational and environmental toxins on sperm.

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“…[28]. The later two studies performed among the same group of Taiwanese workers found that urinary 1-OHP does not significantly correlate with semen quality; however, PAHs with heavy molecular weight, e.g., benzo(g,h,i)perylene and benzo(k)fluoranthene, negatively correlate with morphology and motility of sperm cells (p = 0.02 and 0.002, p = 0.04 and 0.04, respectively) [39]. The second study by did not find any significant differences in sperm concentrations, vitality and DNA fragmentation between the topside oven workers (exposed) and the side oven workers (unexposed) [40].…”

Section: Ijomeh 2013;26(5) 797mentioning

confidence: 99%

Association between a biomarker of exposure to polycyclic aromatic hydrocarbons and semen quality

Jurewicz¹,

Radwan²,

Sobala³

et al. 2013

International Journal of Occupational Medicine and Environmental Health

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Objectives: Growing evidence supports the reproductive and developmental toxicity of polycyclic aromatic hydrocarbons (PAHs) from prenatal and postnatal exposure, but the results of epidemiological studies regarding harmful effects of PAHs exposure on male reproductive system still remain limited and inconclusive. The aim of the present study was to investigate the relationship between 1-hydroxypyrene, a biomarker of polycyclic aromatic hydrocarbons exposure and semen quality. Materials and Methods:The study population consisted of 277 men attending an infertility clinic for diagnostic purposes and having normal semen concentration of 20-300 mln/ml or slight oligozoospermia (semen concentration: 15-20 mln/ml) (WHO 1999). All the men were healthy and under 45 years of age. All participants were interviewed and provided a semen sample. The interview included questions concerning demographics, socio-economic status, medical history related to past diseases which may have an impact on semen quality, lifestyle factors and occupational information. Concentrations of 1-hydroxypyrene (1-OHP) in the urine samples were analyzed using high performance liquid chromatography (HPLC). Results: A positive association was found between the level of 1-OHP in urine and sperm neck abnormalities as well as the percentage of static sperm cells (p = 0.001, p = 0.018, respectively). Additionally, exposure to PAHs measured by 1-OHP in urine decreased semen volume and the percentage of motile sperm cells (p = 0.014, p = 0.0001, respectively). Conclusions: Presented findings indicate that the environmental level of PAHs exposure adversely affects male semen quality. The future large-scale studies should incorporate different biomarkers to generate a more accurate and full assessment of the effects of PAHs exposure on male fertility.

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1-Hydroxypyrene as a biomarker for assessing the effects of exposure to polycyclic aromatic hydrocarbons on semen quality and sperm DNA integrity

Cited by 27 publications

References 43 publications

Abnormalities in the male reproductive system after exposure to diesel and biodiesel blend

Abnormalities in the male reproductive system after exposure to diesel and biodiesel blend

Sperm DNA oxidative damage and DNA adducts

Association between a biomarker of exposure to polycyclic aromatic hydrocarbons and semen quality

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