1-ethyl-3-methyl imidazolium acetate, hemicellulolytic enzymes and laccase-mediator system: Toward an integrated co-valorization of polysaccharides and lignin from Miscanthus

Revisiting organosolv strategies for sustainable extraction of valuable lignin: the CoffeeCat process

“…They also support the current idea in the literature that enzymatic digestibility is not solely related to the lignin content, but also to the disruption of the hydrogen bond network and to some extent to the cellulose crystallinity 42 or to the presence of impurities and potential inhibitors. To this end, ionic liquids can be used 103–105 to overcome the persistent recalcitrance of the biomass. 25–28 It was thus chosen to perform enzymatic hydrolysis after an additional mild pretreatment, the classically used 1-ethyl-3-methylimidazolium acetate ([Emim][OAc])-based pretreatment.…”

A novel green biorefinery strategy for corn stover by pretreatment with weak alkali-assisted deep eutectic solvents

“…This difference is attributed to the removal of most p-coumarate esters ( pCE) attached to the γ-site of the lignin molecule. 66 Previous studies have shown that an increase in phenolic hydroxyl groups in lignin could improve unproductive binding and inhibition of enzymes. 62,67 Because of their higher electrophilicity and lower spatial site resistance, H-OH groups are the dominant phenolic hydroxyl groups and exhibit the highest inhibition and unproductive adsorption.…”

Upstream and Downstream Bioprocessing in Enzyme Technology

“…Depending on the polarity of the substrates, green solvents that can dissolve the substrates can be designed. Among green solvents are ionic liquids (ILs) [ 60 , 61 ], DESs [ 62 , 63 ], and bio-based solvents [ 64 , 65 , 66 ]. ILs are formed by a cation and an anion, are complex to prepare, and are expensive.…”