1 Cellular protein TTC4 and its cofactor HSP90 are pro-viral for bovine herpesvirus 1

Thompson, Beth; Dry, Inga; Dalziel, Robert; Gaunt, Eleanor

doi:10.1016/j.virusres.2022.198927

Cited by 3 publications

(1 citation statement)

References 40 publications

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“…Infectious bovine rhinotracheitis virus (IBRV) or bovine herpesvirus type-1 (BoHV-1), is a double-stranded DNA virus belonging to the family Herpesviridae, subfamily Alphaherpesvirinae [ 1 ]. IBRV infection causes various cattle diseases, such as rhinitis, encephalitis, conjunctivitis, and vulvovaginitis [ 2 , 3 ]. It can also lead to abortion and immunosuppression, leading to more serious diseases.…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells

Ma,

Guo,

et al. 2024

BMC Genomics

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Background Studies have confirmed that Infectious bovine rhinotracheitis virus (IBRV) infection induces mitochondrial damage. MicroRNAs (miRNAs) are a class of noncoding RNA molecules, which are involved in various biological processes and pathological changes associated with mitochondrial damage. It is currently unclear whether miRNAs participate in IBRV-induced mitochondrial damage in Madin-Darby bovine kidney (MDBK) cells. Results In the present study, we used high-throughput sequencing technology, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to screen for mitochondria-related miRNAs and messenger RNAs (mRNAs). In total, 279 differentially expressed miRNAs and 832 differentially expressed mRNAs were identified in 6 hours (IBRV1) versus 24 hours (IBRV2) after IBRV infection in MDBK cells. GO and KEGG enrichment analysis revealed that 42 differentially expressed mRNAs and 348 target genes of differentially expressed miRNAs were correlated with mitochondrial damage, and the miRNA-mitochondria-related target genes regulatory network was constructed to elucidate their potential regulatory relationships. Among the 10 differentially expressed miRNAs, 8 showed expression patterns consistent with the high-throughput sequencing results. Functional validation results showed that overexpression of miR-10a and miR-182 aggravated mitochondrial damage, while inhibition of miR-10a and miR-182 alleviated mitochondrial damage. Conclusions This study not only revealed the expression changes of miRNAs and mRNAs in IBRV-infected MDBK cells, but also revealed possible biological regulatory relationship between them. MiR-10a and miR-182 may have the potential to be developed as biomarkers for the diagnosis and treatment of IBRV. Together, Together, these data and analyses provide additional insights into the roles of miRNA and mRNA in IBRV-induced mitochondria damage

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Section: Introductionmentioning

confidence: 99%

Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells

Ma,

Guo,

et al. 2024

BMC Genomics

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Bacterial induced miR-144-5p modulates intestinal inflammatory response of Japanese flounder by targeting Hsp90α-dependent NLR signaling pathway

Lin²,

Guo³

et al. 2023

Aquaculture

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Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells

Guo

et al. 2023

Preprint

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Background Studies have confirmed that Infectious bovine rhinotracheitis virus (IBRV) infection induces mitochondrial damage. MicroRNAs (miRNAs) are a class of noncoding RNA molecules, which are involved in various biological processes and pathological changes associated with mitochondrial damage. It is not known whether miRNAs participate in IBRV-induced mitochondrial damage in Madin-Darby bovine kidney (MDBK) cells. Results In the present study, we used high-throughput sequencing technology, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to screen mitochondria-related miRNAs and messenger RNAs (mRNAs). In total, 279 differentially expressed miRNAs and 832 differentially expressed mRNAs were identified in 6 hours (IBRV1) versus 24 hours (IBRV2) after IBRV infection in MDBK cells. GO and KEGG enrichment analysis revealed that 42 differentially expressed mRNAs and 348 target genes of differentially expressed miRNAs were correlated with mitochondrial damage, and the miRNA-mitochondria-related target genes regulatory network was constructed to elucidate their underlying regulatory relationships. The expression patterns of eight of ten differentially expressed miRNAs were consistent with the high-throughput sequencing results, Functional validation results showed that the overexpression of miR-10a and miR-182 aggravated mitochondrial damage and that the inhibition of miR-10a and miR-182 alleviated mitochondrial damage. Conclusions This study revealed not only the expression changes of miRNAs and mRNAs in IBRV-infected MDBK cells but also the possible biological regulatory relationship between them. MiR-10a and miR-182 have the potential to be developed as biomarkers for IBRV diagnosis and treatment. Together, these data and analyses provide additional insight into the role of miRNAs and mRNAs in IBRV-induced mitochondria damage.

show abstract

1 Cellular protein TTC4 and its cofactor HSP90 are pro-viral for bovine herpesvirus 1

Cited by 3 publications

References 40 publications

Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells

Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells

Bacterial induced miR-144-5p modulates intestinal inflammatory response of Japanese flounder by targeting Hsp90α-dependent NLR signaling pathway

Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells

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