1-Aminocyclopropane-1-Carboxylate (ACC) Deaminase Genes in Rhizobia from Southern Saskatchewan

Duan, Jingliang; Müller, Kirsten M.; Charles, Trevor C.; Vesely, Susanne; Glick, Bernard R.

doi:10.1007/s00248-008-9407-6

Cited by 177 publications

(66 citation statements)

References 57 publications

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“…Thus, biofilm forming bacteria may protect plants using the protective biofilm layer by mechanisms such as niche exclusion [25], [26], [27]. This ability of bacteria to protect both themselves and their plant hosts may be synergistically strengthened by inducing the basic protection of enhancing homeostasis via ACC breakdown [12], [14], [28], [29].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Distribution in the Rhizosphere of Wild Barley under Contrasting Microclimates

et al. 2011

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BackgroundAll plants in nature harbor a diverse community of rhizosphere bacteria which can affect the plant growth. Our samples are isolated from the rhizosphere of wild barley Hordeum spontaneum at the Evolution Canyon (‘EC’), Israel. The bacteria which have been living in close relationship with the plant root under the stressful conditions over millennia are likely to have developed strategies to alleviate plant stress.Methodology/Principal FindingsWe studied distribution of culturable bacteria in the rhizosphere of H. spontaneum and characterized the bacterial 1-aminocyclopropane-1-carboxylate deaminase (ACCd) production, biofilm production, phosphorus solubilization and halophilic behavior. We have shown that the H. spontaneum rhizosphere at the stressful South Facing Slope (SFS) harbors significantly higher population of ACCd producing biofilm forming phosphorus solubilizing osmotic stress tolerant bacteria.Conclusions/SignificanceThe long-lived natural laboratory ‘EC’ facilitates the generation of theoretical testable and predictable models of biodiversity and genome evolution on the area of plant microbe interactions. It is likely that the bacteria isolated at the stressful SFS offer new opportunities for the biotechnological applications in our agro-ecological systems.

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Section: Discussionmentioning

confidence: 99%

Bacterial Distribution in the Rhizosphere of Wild Barley under Contrasting Microclimates

et al. 2011

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“…AcdS , the structural gene encoding ACCD was amplified by polymerase chain reaction (PCR) using universal primers (Duan et al, 2009). Sequences of primers were: (F) 5′-GGCAAGGTCGACATCTATC-3′ and (R) 5′-GGCTTGCCATTCAGCTATG-3′.…”

Section: Methodsmentioning

confidence: 99%

A Halotolerant Bacterium Bacillus licheniformis HSW-16 Augments Induced Systemic Tolerance to Salt Stress in Wheat Plant (Triticum aestivum)

Singh¹,

Jha²

2016

Front. Plant Sci.

113

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Certain plant growth promoting bacteria can protect associated plants from harmful effects of salinity. We report the isolation and characterization of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase bacterium Bacillus licheniformis HSW-16 capable of ameliorating salt (NaCl) stress in wheat plants. The bacterium was isolated from the water of Sambhar salt lake, Rajasthan, India. The presence of ACC deaminase activity was confirmed by enzyme assay and analysis of AcdS gene, a structural gene for ACC deaminase. Inoculation of B. licheniformis HSW-16 protected wheat plants from growth inhibition caused by NaCl and increased plant growth (6-38%) in terms of root length, shoot length, fresh weight, and dry weight. Ionic analysis of plant samples showed that the bacterial inoculation decreased the accumulation of Na+ content (51%), and increased K+ (68%), and Ca2+ content (32%) in plants at different concentration of NaCl. It suggested that bacterial inoculation protected plants from the effect of NaCl by decreasing the level of Na+ in plants. Production of exopolysaccharide by the B. licheniformis HSW-16 can also protect from Na+ by binding this ion. Moreover, application of test isolate resulted in an increase in certain osmolytes such as total soluble sugar, total protein content, and a decrease in malondialdehyde content, illustrating their role in the protection of plants. The ability of B. licheniformis HSW-16 to colonize plant root surface was examined by staining the bacterium with acridine orange followed by fluorescence microscopy and polymerase chain reaction-based DNA finger printing analysis. These results suggested that B. licheniformis HSW-16 could be used as a bioinoculant to improve the productivity of plants growing under salt stress.

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“…The result of lowering the level of ethylene in legume hosts is that both the number of nodules and the biomass of the plant may be increased by 25–40% [32, 33]. In the field, approximately 1–10% of rhizobial strains naturally possess ACC deaminase [34] thus it is possible to increase the nodulation efficiency of Rhizobia strains that lack ACC deaminase by engineering these strains with Rhizobia ACC deaminase genes (and regulatory regions) isolated from other strains. In one instance, insertion of an ACC deaminase gene from R. leguminosarum bv.…”

Section: Direct Mechanismsmentioning

confidence: 99%

Plant Growth-Promoting Bacteria: Mechanisms and Applications

2012

Self Cite

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The worldwide increases in both environmental damage and human population pressure have the unfortunate consequence that global food production may soon become insufficient to feed all of the world's people. It is therefore essential that agricultural productivity be significantly increased within the next few decades. To this end, agricultural practice is moving toward a more sustainable and environmentally friendly approach. This includes both the increasing use of transgenic plants and plant growth-promoting bacteria as a part of mainstream agricultural practice. Here, a number of the mechanisms utilized by plant growth-promoting bacteria are discussed and considered. It is envisioned that in the not too distant future, plant growth-promoting bacteria (PGPB) will begin to replace the use of chemicals in agriculture, horticulture, silviculture, and environmental cleanup strategies. While there may not be one simple strategy that can effectively promote the growth of all plants under all conditions, some of the strategies that are discussed already show great promise.

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1-Aminocyclopropane-1-Carboxylate (ACC) Deaminase Genes in Rhizobia from Southern Saskatchewan

Cited by 177 publications

References 57 publications

Bacterial Distribution in the Rhizosphere of Wild Barley under Contrasting Microclimates

Bacterial Distribution in the Rhizosphere of Wild Barley under Contrasting Microclimates

A Halotolerant Bacterium Bacillus licheniformis HSW-16 Augments Induced Systemic Tolerance to Salt Stress in Wheat Plant (Triticum aestivum)

Plant Growth-Promoting Bacteria: Mechanisms and Applications

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