1′-Acetoxychavicol Acetate from Alpinia galanga Represses Proliferation and Invasion, and Induces Apoptosis via HER2-signaling in Endocrine-Resistant Breast Cancer Cells

Pradubyat, Nalinee; Giannoudis, Athina; Elmetwali, Taha; Mahalapbutr, Panupong; Palmieri, Carlo; Mitrpant, Chalermchai; Ketchart, Wannarasmi

doi:10.1055/a-1307-3997

Cited by 6 publications

(8 citation statements)

References 47 publications

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“…The resulting IC 50 value was 2 g/ml [31]. ACA can also be an alternative inhibitor for uPA and FGF2 because it can bind to them through a hydrogen formation pattern [32].…”

Section: Resultsmentioning

confidence: 99%

Systematic Review: Anticancer Potential of Active Compounds from Galangal (Alpinia galanga)

Suciati¹,

Maryati²

2022

Atlantis Highlights in Chemistry and Pharmaceutical Sciences

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Galangal (Alpinia galanga) is a plant that is widely spread in Southeast Asia and is widely used for cooking spices. Research related to the effect of A. galanga on cancer cells has been carried out. The purpose of this systematic review is to examine the potential of A. galanga as an anticancer agent and co-chemotherapy. The literature search was carried out through the Pubmed, Science Direct, Springer Link, DOAJ, and Garuda Portal databases. The keywords used were "Alpinia Galanga and Anticancer", "Alpinia Galanga and Cytotoxicity", "Lengkuas dan Sitotoksik", "Lengkuas dan Kanker", and "Alpinia Galanga and Cancer". Of the 965 articles found at the beginning of the search, 31 articles were obtained which had been filtered and discussed further. The results showed that the ethanolic extract of A. galanga had a cytotoxic effect because it contained antioxidant polyphenols that inhibited cell proliferation by stopping the cell cycle. The most abundant compounds in A. galanga as anticancer agents are 1'-Acetoxychavicol Acetate (ACA) and Galangin. ACA works to inhibit cancer cells by inducing apoptosis through mitochondrial depolarization and DNA fragmentation in the cell cycle phase. Meanwhile, Galangin works as a chemopreventive agent that reduces oxidative stress and inflammation by suppressing the MAPKs pathway.

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“…The resulting IC 50 value was 2 g/ml [31]. ACA can also be an alternative inhibitor for uPA and FGF2 because it can bind to them through a hydrogen formation pattern [32].…”

Section: Resultsmentioning

confidence: 99%

Systematic Review: Anticancer Potential of Active Compounds from Galangal (Alpinia galanga)

Suciati¹,

Maryati²

2022

Atlantis Highlights in Chemistry and Pharmaceutical Sciences

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“…specimen CP-Ag-29) . Pradubyat et al (2022) and the team approved the extraction and purification of ACA in 2022 and resisted the patent (Pub. No.…”

Section: ′-Acetoxychavicol Acetate Bioactive Compoundmentioning

confidence: 99%

“…The techniques mentioned above were replicated in three separate experiments to confirm the reliability of the results. The cell viability was calculated from the ratio of mean absorbance treated cells to mean absorbance control cells (Pradubyat et al, 2022).…”

Section: The Cytotoxicity Activities Testing (Viability Assay)mentioning

confidence: 99%

“…Previous studies discovered synergistic anticancer effects by combining ACA with standard chemotherapy drugs on cervix carcinoma cells ( Phuah et al , 2017) . Pradubyat et al (2022) and team found the effects of ACA on breast cancer cell lines ( MCF7, MCF7/ LCC2, MCF7/ LCC9) included inhibiting cell proliferation through the downregulation of HER2 receptor, estrogen receptor coactivator ( NCOA3) , pERK1/ 2, pAKT, CCND1, C-myc (Pradubyat et al, 2022). ACA was shown to inhibit cell proliferation of SW480 colorectal adenocarcinoma with an IC50 of 80 µM after 48 hours.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of 1'-acetoxychavicol Acetate on A549 Human Non-small Cell Lung Cancer

Ketkomol,

Songsak,

Jongrungruangchok

et al. 2024

JCST

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Lung cancer, a global leading cause of cancer mortality, requires safer and more effective treatments. 1'-acetoxychavicol acetate (ACA) is notable for its wide-ranging therapeutic properties, spanning anticancer, anti-inflammatory, and anti-obesity effects. While its efficacy has been explored in various cell lines, its potential against lung cancer remains few reported. This research aimed to investigate the impact of ACA on cytotoxicity, anti-proliferation, and antimigration potential in A549 human non-small cell lung cell lines. The investigation utilized MTT assays to assess cell viability and determined the IC50 values of ACA at 24, 48, and 72 hours to be 50.42 µM, 33.22 µM, and 21.66 µM, respectively. These results reveal a concentration- and time-dependent reduction in cell viability following ACA treatment. In addition, anti-proliferation was performed using colony-forming assays, and ACA showed notable efficacy in significantly decreasing colony formation, highlighting its strong impact on cell proliferation and viability. In the final part, ACA had promising inhibitory effects on migration, which varied depending on the dose used in the scratch assay. In conclusion, ACA highlighted cytotoxic and anti-proliferation properties of A549 cell line, supporting its potential role in lung cancer therapy pending further investigation and development.

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“…In particular, the inhibitory effect of ACA on carcinogenesis has been demonstrated in several animal models of various tumor types, including colon, liver, esophageal, skin, and prostate cancers [ 17 , 18 , 19 , 20 , 21 ]. Moreover, ACA has been shown to induce apoptosis and cell cycle arrest and inhibit angiogenesis and invasion in several cancer cell lines [ 22 , 23 ]. However, little is known regarding the effects of 1′-acetoxyeugenol acetate (AEA), another active compound in A. galanga , on human cancer.…”

Section: Introductionmentioning

confidence: 99%

1′-Acetoxyeugenol Acetate Isolated from Thai Ginger Induces Apoptosis in Human Ovarian Cancer Cells by ROS Production via NADPH Oxidase

et al. 2022

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The rhizomes of Alpinia galanga (Thai ginger) have been used extensively as a spice in Southeast Asian and Arabian cuisines and reported to possess a wide range of biological properties, such as antioxidant, antimicrobial, and antibacterial. However, the specific molecular and cellular mechanisms underlying the anti-tumor effects induced by Thai ginger and its corresponding active compounds have been poorly characterized. We found that upon EtOH extraction, Thai ginger extract exhibits cytotoxic activity (IC50 < 10 μg/mL) and triggers cell death via caspase-dependent apoptosis in human ovarian cancer cells. Among the three major compounds isolated from the extract, 1′-acetoxyeugenol acetate (AEA) exhibited potent cytotoxic activity in human ovarian cancer cells, SKOV3 and A2780. AEA induced apoptotic cell death through the activation of caspases-3 and -9. Notably, AEA enhanced the intracellular levels of reactive oxygen species (ROS), and the application of an antioxidant markedly reversed AEA-induced apoptosis of ovarian cancer cells. The knockdown of p47phox, a subunit of NADPH oxidase, suppressed both the pro-apoptotic and ROS-inducing effects of AEA. Additionally, the activation of the mitogen-activated protein kinase (MAPK) pathway by AEA through ROS regulation was found to be involved in AEA-induced apoptosis. Altogether, these results suggest that AEA exhibits potent apoptosis-inducing activity through the activation of the intrinsic pathway via ROS-mediated MAPK signaling in human ovarian cancer cells.

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1′-Acetoxychavicol Acetate from Alpinia galanga Represses Proliferation and Invasion, and Induces Apoptosis via HER2-signaling in Endocrine-Resistant Breast Cancer Cells

Cited by 6 publications

References 47 publications

Systematic Review: Anticancer Potential of Active Compounds from Galangal (Alpinia galanga)

Systematic Review: Anticancer Potential of Active Compounds from Galangal (Alpinia galanga)

The Effect of 1'-acetoxychavicol Acetate on A549 Human Non-small Cell Lung Cancer

1′-Acetoxyeugenol Acetate Isolated from Thai Ginger Induces Apoptosis in Human Ovarian Cancer Cells by ROS Production via NADPH Oxidase

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