1,7-Dihydroxy-3,4-Dimethoxyxanthone Inhibits Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages by Suppressing TLR4/NF-κB Signaling Cascades

Tao, Meng-Qing; Ji, Cong‐Lan; Wu, Youliang; Dong, Jiyang; Li, Yan; Olatunji, Opeyemi Joshua; Zuo, Jian

doi:10.1007/s10753-020-01256-3

Cited by 17 publications

(7 citation statements)

References 29 publications

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“…3A, while LPS stimulation led to a significant increase in PGE 2 production, this effect was inhibited by pretreatment with FC. Likewise, we observed that LPS stimulation increased the expression of COX-2, a key enzyme of PGE biosynthesis [17], while FC significantly suppressed LPS-stimulated up-regulation of COX-2 at both mRNA and protein levels (Fig. 3B, C).…”

Section: Effects Of Fc On Lps-elicited Cox-2 Expression and Pge Productionsupporting

confidence: 60%

Formosanin C attenuates lipopolysaccharide-induced inflammation through nuclear factor-κB inhibition in macrophages

Liu

Shi

Zhang

et al. 2021

Korean J Physiol Pharmacol

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Section: Effects Of Fc On Lps-elicited Cox-2 Expression and Pge Productionsupporting

confidence: 60%

Formosanin C attenuates lipopolysaccharide-induced inflammation through nuclear factor-κB inhibition in macrophages

Liu

Shi

Zhang

et al. 2021

Korean J Physiol Pharmacol

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“…In 2008, Hirata et al showed that LPS can induce an increase in inflammatory and anti-inflammatory cytokines as long as there is a synergy between TLR-4 ligands, such as LPS, and TLR-2 ligands [84], and possibly there is an interaction in their signalling pathways. Alternatively, in a way that is still unknown, BB extract could inhibit TLR-4 and consequently the activity of LPS, as described for other plant extracts [85].…”

Section: Discussionmentioning

confidence: 96%

Blueberry Counteracts BV-2 Microglia Morphological and Functional Switch after LPS Challenge

et al. 2020

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Microglia, the innate immune cells of the CNS, respond to brain injury by activating and modifying their morphology. Our study arises from the great interest that has been focused on blueberry (BB) for the antioxidant and pharmacological properties displayed by its components. We analyzed the influence of hydroalcoholic BB extract in resting or lipopolysaccharide (LPS)-stimulated microglia BV-2 cells. BB exerted a protective effect against LPS-induced cytotoxicity, as indicated by cell viability. BB was also able to influence the actin cytoskeleton organization, to recover the control phenotype after LPS insult, and also to reduce LPS-driven migration. We evaluated the activity of Rho and Rac1 GTPases, which regulate both actin cytoskeletal organization and migratory capacity. LPS caused an increase in Rac1 activity, which was counteracted by BB extract. Furthermore, we demonstrated that, in the presence of BB, mRNA expression of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α decreased, as did the immunofluorescence signal of iNOS, whereas that of Arg-1 was increased. Taken together, our results show that, during the inflammatory response, BB extract shifts the M1 polarization towards the M2 phenotype through an actin cytoskeletal rearrangement. Based on that, we might consider BB as a nutraceutical with anti-inflammatory activities.

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“…The TLR4/NF-κB signaling pathway is a significant control center for inflammation regulation and an essential target for the discovery of new drugs to treat various inflammatory diseases. Tao et al (2020) identified that inhibition of the TLR4/NF-κB signaling pathway suppressed LPS-induced inflammation in RAW264.7 macrophages. To investigate the deeper mechanism of the anti-inflammatory activity of LBP, the expression of the deeper mechanisms of TLR4, NF-κB P65, and iNOS proteins were investigated using Western blotting and RT-PCR.…”

Section: Discussionmentioning

confidence: 99%

Lycium barbarum polysaccharides ameliorate LPS‐induced inflammation of RAW264.7 cells and modify the behavioral score of peritonitis mice

Liu

Zhou

et al. 2021

J Food Biochem

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In the present study, the anti-inflammatory effect of Lycium barbarum polysaccharide (LBP) and the possible molecular mechanism thereof were examined, so as to perceive the pharmacological action of LBP. With acute peritonitis in mice as the inflammatory model, the protective effect of LBP on peritonitis mice was evaluated by recording the effect of behavioral scores, studying the pathological damage of intestine and liver, and detecting the levels of inflammatory cytokines. Additionally, by establishing an lipopolysaccharide (LPS)-induced RAW264.7 macrophage model, the effect of LBP on RAW264.7 cell phenotype and culture supernatant inflammatory markers was observed. Finally, the activation of inflammation-related target genes, such as iNOS, Toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB) p65, and IκBα, were further detected. The results reveal that pretreatment with LBP could decrease the behavioral score of inflammatory mice, inhibit the secretion of pro-inflammatory factors, and reduce liver and intestine injury. LBP can regulate the effect of lipopolysaccharide on the polarization of RAW264.7 cells, and reduce the production of NO and cytokines (TNFα, IL-1β, IL-6). Further, LBP pretreatment was found to be able to significantly reduce the expression of iNOS, TLR4, NF-κB p65, and IκBα in macrophages. The present research provides evidence that LBP exerts potential antiinflammatory activity in LPS-induced RAW264.7 macrophages via inhibiting TLR4 and NF-κB inflammatory sites and improving the behavior score of peritonitis mice. Practical applicationsIn recent years, the number of deaths worldwide has continued to rise as a result of inflammation. Despite said rise in deaths, many synthetic drugs with anti-inflammatory properties are significantly expensive and also have a host of side effects. Thus, the development of new anti-inflammatory drugs derived from medicinal plants has broad application potential. As such, in the present study, lipopolysaccharide (LPS)induced macrophages were used to establish inflammatory cell models to verify the anti-inflammatory effect of Lycium barbarum polysaccharides (LBP). Findings were made that LBP could reduce the expression levels of inflammatory cytokines and NO by regulating macrophage polarization and NF-κB translocation, and thus, could exert anti-inflammatory activity.

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1,7-Dihydroxy-3,4-Dimethoxyxanthone Inhibits Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages by Suppressing TLR4/NF-κB Signaling Cascades

Cited by 17 publications

References 29 publications

Formosanin C attenuates lipopolysaccharide-induced inflammation through nuclear factor-κB inhibition in macrophages

Formosanin C attenuates lipopolysaccharide-induced inflammation through nuclear factor-κB inhibition in macrophages

Blueberry Counteracts BV-2 Microglia Morphological and Functional Switch after LPS Challenge

Lycium barbarum polysaccharides ameliorate LPS‐induced inflammation of RAW264.7 cells and modify the behavioral score of peritonitis mice

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