“…This review systematically summarizes various types of ratiometric UCL nanoprobes, referring to the primary structures and compositions of the nanoprobe systems, dual-signal ratiometric modes, sensing mechanisms, and application performances. For convenient mastering, previous studies on ratiometric UCL nanoprobes are appropriately classified and regularly arranged based on specific targets and characters, including temperature (Table 1), 28–84 pH (Table 2), 85–99 inorganic anions, cations, free radicals (Table 3), 100–126 gases, small common molecules, biological small molecules and biomacromolecules (Table 4). 127–162 For a better understanding of construction strategies, the following sections provide principle construction methods and ratiometric dual-signal modes of ratiometric UCL nanoprobes by discussing the state-of-the-art related studies.…”