1,3-β-D glucan binding protein (BGBP) from the white shrimp, Penaeus vannamei, is also a heparin binding protein

Biophysical Characterization of the Non-Fusogenic Interaction Between Liposomes and the Shrimp Bifunctional Lipoprotein-β-Glucan Binding Protein HDL/BGBP

“…In previous work, HDL-BGBP was purified by heparin affinity chromatography [15], however by this combined method we obtained good yields of HDL-BGBP and the buoyant density was 1.19 g/ml as reported before. Purified HDL-BGBP was observed as a band of ~ 100 kDa detected by SDS-PAGE, Fig.…”

“…Plasma was centrifuged at 1,000 x g to remove blood cells (hemocytes) and precipitated by dialysis against distilled water as previously described [15]. The pellet was resuspended in buffer 50 mM Tris-HCl (pH 7.5) and subjected to density gradient ultracentrifugation in a similar procedure as previously described [16].…”

“…HDL-BGBP was purified by a combination of protein precipitation [15] and ultracentrifugation [1,16] methods. In previous work, HDL-BGBP was purified by heparin affinity chromatography [15], however by this combined method we obtained good yields of HDL-BGBP and the buoyant density was 1.19 g/ml as reported before.…”

Discovery of genes expressed in response to Perkinsus marinus challenge in Eastern (Crassostrea virginica) and Pacific (C. gigas) oysters

“…Surprisingly, no lectin-coding gene was identified in C. gigas libraries but one gene, beta-1,3-glucan binding protein, was identified. This protein is thought to play an important role in the innate immune response of crustaceans and insects because binding of lipopolysaccharide and beta-1,3-glucan binding protein activates the prophenoloxidase (proPO) cascade (Jimenez-Vega et al, 2002). This beta-1,3-glucan binding protein gene is also known to be up-regulated by bacterial and fungal infections in shrimp and the protein produced could be an inducible acute-phase protein that may play a critical role in host-parasite interactions (Roux et al, 2002).…”

Purification and characterization of a β-glucan binding protein from the haemolymph of freshwater prawnMacrobrachium rosenbergii

“…βGBP from M. rosenbergii serum was purified by affinity chromatography on heparin column following the method of Jimenez‐Vega, Sotelo‐Mundo, Ascencio and Vargas‐Albores () with minor modifications. In brief, 2 mL of HEPARIN HYPER D sorbent (PALL Life Sciences, Port Washington, NY, USA) was packed in a small glass column (C1708, Genei, Bengaluru, India) and equilibrated with Tris citrate buffer (0.05 M Tris citrate with 0.15 M NaCl, pH 7.4).…”