1,3‐Propanediol

Sun, Yaqin; Ma, Chengwei; Fu, Hongxin; Mu, Ying; Xiu, Zhilong

doi:10.1002/9781118845394.ch11

Cited by 8 publications

(3 citation statements)

References 133 publications

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“…An interesting example is 2-amino-4-hydroxybutanoic acid (i.e., homoserine), a noncanonical amino acid homologous to l -serine by insertion of an additional methylene unit into the side chain and an important metabolic intermediate . It also constitutes a valuable building block for the synthesis of 3-hydroxypropionic acid and 1,3-propanediol, important starting materials in the manufacture of biocompatible plastic and polytrimethylene terephthalate (PTT), among other industrial products . Furthermore, l -homoserine lactone derivatives are useful compounds for studying the quorum sense signaling (QSS) mechanism (i.e., cell to cell communication) and indeed as potential QSS inhibitors in new antimicrobial strategies against pathogenic Gram-negative and Gram-positive bacteria (antivirulence intervention strategy). , …”

Section: Introductionmentioning

confidence: 99%

Combining Aldolases and Transaminases for the Synthesis of 2-Amino-4-hydroxybutanoic Acid

et al. 2017

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Amino acids are of paramount importance as chiral building blocks of life, for drug development in modern medicinal chemistry, and for the manufacture of industrial products. In this work, the stereoselective synthesis of (S)-and (R)-2-amino-4-hydroxybutanoic acid was accomplished using a systems biocatalysis approach comprising a biocatalytic one-pot cyclic cascade by coupling of an aldol reaction with an ensuing stereoselective transamination. A class II pyruvate aldolase from E. coli, expressed as a soluble fusion protein, in tandem with either an S-or R-selective, pyridoxal phosphate dependent transaminase was used as a catalyst to realize the conversion, with formaldehyde and alanine being the sole starting materials. Interestingly, the class II pyruvate aldolase was found to tolerate formaldehyde concentrations of up to 1.4 M. The cascade system was found to reach product concentrations for (S)-or (R)-2-amino-4-hydroxybutanoic acid of at least 0.4 M, rendering yields between 86% and >95%, respectively, productivities of >80 g L −1 d −1 , and ee values of >99%.

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Section: Introductionmentioning

confidence: 99%

Combining Aldolases and Transaminases for the Synthesis of 2-Amino-4-hydroxybutanoic Acid

et al. 2017

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“…Furthermore, a 1,3-PDO concentration of 28.26 g L –1 was achieved after 16 h of glycerol fermentation in a 5 L batch bioreactor. The separation of 1,3-PDO poses a formidable challenge in scaling up its industrial production due to the complexity of fermentation broths, which can contain residual amounts of glycerol, macromolecules (such as proteins, polysaccharides, and nucleic acids), and products of cellular metabolism. − …”

Section: Introductionmentioning

confidence: 99%

Salting-out as a Fast and Simple Method for First-Step Separation of Biobased 1,3-Propanediol

Liberato,

Buarque,

Ferreira

et al. 2024

J. Chem. Eng. Data

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The cost of 1,3-propanediol (1,3-PDO) microbial production is very much dependent on the separation of 1,3-PDO from the fermentation broth. The use of byproducts as nutrient sources has significant economic and environmental advantages. This specific molecule can be produced from the fermentation of raw glycerol. Crude glycerol is a primary byproduct in the biodiesel production process, and its large surplus blocks the development of the biodiesel industry. This study focused on separating 1,3-PDO through salting-out extraction (SOE), employing ethanol and 2propanol, along with five different salts, for screening. Subsequently, an experimental design was implemented to determine optimal alcohol and salt concentrations, maximizing key variables such as the partition coefficient and recovery yield. The entire quantity of 1,3-PDO was successfully recovered in the ethanolic phase, exhibiting a selectivity of 4.87 concerning glycerol. This achievement was realized by using 39 wt % KH 2 PO 4 and 19 wt % ethanol. According to the remarkable results, salting-out extraction based on ethanol and K 2 HPO 4 can be considered as an alternative and efficient platform for separating 1,3-PDO from glycerol, selectively obtaining two compounds with a high added value.

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“…l -Homoserine, synthesized in this work, is a noncanonical amino acid, a precursor of essential amino acids such as l -threonine, l -methionine, and l -isoleucine. − It is also a valuable building block for the synthesis of 3-hydroxypropionaldehyde and 1,3-propanediol − and has potential applications as a fertilizer and feed additive. , Several examples in the literature − reported the metabolic engineering of microorganisms for the production of l -homoserine. Metabolic engineering implies the use of growing cells as catalysts, which basically means that the biocatalyst is produced simultaneously as the reaction is carried out .…”

Section: Introductionmentioning

confidence: 99%

Cascade Synthesis of l-Homoserine Catalyzed by Lyophilized Whole Cells Containing Transaminase and Aldolase Activities: The Mathematical Modeling Approach

Katulić

Sudar

Hernández

et al. 2021

Ind. Eng. Chem. Res.

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Aldolase and transaminase coexpressed in Escherichia coli cells and lyophilized (i.e., lyophilized whole-cell biocatalyst (LWCB)) were used as biocatalysts for the one-pot cascade synthesis of L-homoserine with substrate cycling. The kinetic analysis of enzymes within lyophilized cells was performed to evaluate the behavior of the system. The best result among the performed fed-batch reactor experiments achieved was 640.8 mM (76.3 g L −1 ) of L-homoserine with a volume productivity of 2.6 g L −1 h −1 . This is comparable with the results of the same cascade synthesis using cell-free extracts (CFEs) and significantly better than the reports in the literature applying fermentation technology. The approach applied here can serve as guidance for the design of microbial cells with an optimal ratio of expressed enzymes that act as biocatalysts in the cascade, resulting in lower biocatalyst cost, no need for the addition of expensive coenzymes, and enhanced enzyme stability as compared with cell-free extracts.

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1,3‐Propanediol

Cited by 8 publications

References 133 publications

Combining Aldolases and Transaminases for the Synthesis of 2-Amino-4-hydroxybutanoic Acid

Combining Aldolases and Transaminases for the Synthesis of 2-Amino-4-hydroxybutanoic Acid

Salting-out as a Fast and Simple Method for First-Step Separation of Biobased 1,3-Propanediol

Cascade Synthesis of l-Homoserine Catalyzed by Lyophilized Whole Cells Containing Transaminase and Aldolase Activities: The Mathematical Modeling Approach

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