1,3-Propanediol Production Potential by a Locally Isolated Strain of Klebsiella Pneumoniae in Comparison to Clostridium Beijerinckii NRRL B593 from Waste Glycerol

Güngörmüşler, Mine; Gönen, Çağdaş; Azbar, Nuri

doi:10.1007/s10924-011-0326-0

Cited by 13 publications

(14 citation statements)

References 15 publications

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“…Nevertheless, this value is still higher than 7.0 and the data reported in this article also points out that the pH values should be maintained around 7.0. The proposed hypothesis is also supported by previous studies in which a higher 1,3-PDO production was achieved under pH-controlled fermentation conditions (pH = 7) for both microorganisms (Gungormusler et al, 2011). Figure 1 shows the effect of glucose concentration on 1,3-PDO production with K. pneumoniae and C. beijerinckii, respectively.…”

Section: Resultssupporting

confidence: 84%

Comparison of Two Natural 1,3-Propanediol Producers in Regards to the Effect of Glucose Usage as a Co-substrate

Güngörmüşler

Azbar

2015

Energy Sources, Part A: Recovery, Utilization, and Environmenta

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Biodiesel production, in which glycerol is produced as a by-product, has increased significantly and now vast amounts of glycerol have been considered as a waste product. Biotechnological conversion of glycerol into a valuable bioplastic raw material, namely, 1,3-propanediol (1,3-PDO) seems to be very promising. In this study, locally isolated Klebsiella pneumoniae and Clostridium beijerinckii NRRL B593 were comparatively studied for their 1,3-PDO production potential with the addition of different concentrations of glucose together with glycerol. Addition of a co-substrate enhanced the molar yields of 1,3-PDO by 11% only for K. pneumoniae with an addition of 1 g/L of glucose.

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Section: Resultssupporting

confidence: 84%

Comparison of Two Natural 1,3-Propanediol Producers in Regards to the Effect of Glucose Usage as a Co-substrate

Güngörmüşler

Azbar

2015

Energy Sources, Part A: Recovery, Utilization, and Environmenta

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“… Genus Species Reference nos. Klebsiella K. pneumoniae [27] , [15] , [22] , [25] , [33] , [41] , [2] , [17] , [19] , [35] , [16] , [21] , [29] , [18] , [30] K. oxytoca [38] , [39] Clostridium C. butyricum [5] , [11] , [14] , [24] , [36] C. diolis [23] Enterobacter E. agglomerans [4] , [3] , [26] Citrobacter C. freundii [6] , [1] , [8] Lactobacillus L. brevis [9] …”

Section: Introductionmentioning

confidence: 99%

1,3-Propanediol production by Klebsiella oxytoca NRRL-B199 from glycerol. Medium composition and operational conditions

Wojtusik

Rodríguez

Ripoll

et al. 2015

Biotechnology Reports

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“…Moon et al ([ 2010 ]) studied the effects of the impurities in biodiesel-derived glycerol and showed that in spite of having inhibitory effects on cell growth, the impurities only slightly affected volumetric yields, suggesting the effective use of biodiesel-derived glycerol for microbial production of 1,3-PDO. However, regardless of its grade, glycerol concentrations over a certain threshold concentration (approximately 600 mM) negatively impact cell growth rates (Zeng et al [ 1994 ]), reduce glycerol consumption, and reduce 1,3-PDO yields (Gungormusler et al [ 2011a ]). Conversely, greater consumption of glycerol results in greater 1,3-PDO yields.…”

Section: Introductionmentioning

confidence: 99%

Reduced catabolic protein expression in Clostridium butyricum DSM 10702 correlate with reduced 1,3-propanediol synthesis at high glycerol loading

et al. 2014

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Higher initial glycerol loadings (620 mM) have a negative effect on growth and 1,3-propanediol (1,3-PDO) synthesis in Clostridium butyricum DSM 10702 relative to lower initial glycerol concentrations (170 mM). To help understand metabolic shifts associated with elevated glycerol, protein expression levels were quantified by LC/MS/MS analyses. Thirty one (31) proteins involved in conversion of glycerol to 1,3-PDO and other by-products were analyzed by multiple reaction monitoring (MRM). The analyses revealed that high glycerol concentrations reduced cell growth. The expression levels of most proteins in glycerol catabolism pathways were down-regulated, consistent with the slower growth rates observed. However, at high initial glycerol concentrations, some of the proteins involved in the butyrate synthesis pathways such as a putative ethanol dehydrogenase (CBY_3753) and a 3-hydroxybutyryl-CoA dehydrogenase (CBY_3045) were up-regulated in both exponential and stationary growth phases. Expression levels of proteins (CBY_0500, CBY_0501 and CBY_0502) involved in the reductive pathway of glycerol to 1,3-PDO were consistent with glycerol consumption and product concentrations observed during fermentation at both glycerol concentrations, and the molar yields of 1,3-PDO were similar in both cultures. This is the first report that correlates expression levels of glycerol catabolism enzymes with synthesis of 1,3-PDO in C. butyricum. The results revealed that significant differences in the expression of a small subset of proteins were observed between exponential and stationary growth phases at both low and high glycerol concentrations.

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1,3-Propanediol Production Potential by a Locally Isolated Strain of Klebsiella Pneumoniae in Comparison to Clostridium Beijerinckii NRRL B593 from Waste Glycerol

Cited by 13 publications

References 15 publications

Comparison of Two Natural 1,3-Propanediol Producers in Regards to the Effect of Glucose Usage as a Co-substrate

Comparison of Two Natural 1,3-Propanediol Producers in Regards to the Effect of Glucose Usage as a Co-substrate

1,3-Propanediol production by Klebsiella oxytoca NRRL-B199 from glycerol. Medium composition and operational conditions

Reduced catabolic protein expression in Clostridium butyricum DSM 10702 correlate with reduced 1,3-propanediol synthesis at high glycerol loading

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