1,3-Propandiol production by engineered Hansenula polymorpha expressing dha genes from Klebsiella pneumoniae

Hong, Won-Kyung; Kim, Chul‐Ho; Heo, Sun-Yeon; Luo, Lian Hua; Oh, Baek-Rock; Rairakhwada, Dina; Seo, Jeong-Woo

doi:10.1007/s00449-010-0465-z

Cited by 14 publications

(10 citation statements)

References 18 publications

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“…Among these microorganisms, K. pneumoniae is of particular interest due to its flexible regulation of the carbon and reducing equivalent fluxes under different conditions (Zeng et al 1993) as well as its higher product yield and productivity compared to other strains (Hao et al 2008). In K. pneumoniae, glycerol is first converted to 3-hydroxypropionaldehyde (3-HPA) by a coenzyme B12-dependent glycerol dehydratase (DhaB), which is then reduced to 1,3-PD by a reduced nicotinamide adenine dinucleotide (NADH)-dependent 1,3-PD oxidoreductase (DhaT) (Hong et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Enhance 1,3-propanediol production from crude glycerol in batch and fed-batch fermentation with two-phase pH-controlled strategy

Sattayasamitsathit¹,

Methacanon²,

Prasertsan³

2011

Electro Journal of Biotech

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The batch fermentation of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae SU6 at different crude glycerol concentration (40-100 g l -1 ), pH (6.5-7.5) and temperature (31-40ºC) combined with two-phase pH-controlled strategy was investigated. Effect of feeding rate (0.10-0.15 L h -1 ) was studied in fed-batch fermentation. In batch fermentation, the optimal condition was 60 g l -1 crude glycerol, pH control at 6.5 and cultivation temperature at 37ºC. The maximum 1,3-PD of 20 g l -1 , the yield of 0.34 g 1,3-PD g -1 glycerol consumed and the productivity of 1.25 g l -1 h -1 were achieved at 16 hrs cultivation. The by-products were acetic acid and succinic acid at 2.7 and 1.1 g l -1 , respectively. Two-phase pH-controlled strategy gave better results (24.95 g l -1 1,3-PD and 1.78 g l -1 h -1 productivity) than constant pH-controlled strategy (20 g l -1 and 1.25 g l -1 h -1 , respectively) at 16 hrs incubation. In fed-batch fermentation, the maximum 1,3-PD of 45.35 g l -1 was achieved at constant feeding rate of 0.1 L h -1 . The yield and productivity were 0.44 g g -1 and 1.94 g l -1 h -1 , respectively. The fed-batch fermentation with constant feeding at 0.1 L h -1 with two-phase pH-controlled strategy gave 2.2 folds higher 1,3 PD concentration than the batch fermentation with two-phase pH-controlled strategy. This demonstrated the great impact of combination of pH control and feeding strategies in fed-batch fermentation on enhancing 1,3-propanediol production.

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Section: Introductionmentioning

confidence: 99%

Enhance 1,3-propanediol production from crude glycerol in batch and fed-batch fermentation with two-phase pH-controlled strategy

Sattayasamitsathit¹,

Methacanon²,

Prasertsan³

2011

Electro Journal of Biotech

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“…Hong et al co-expressed dhaB gene cluster and dhaT from K. pneumoniae in Hansenula polymorpha [65]. To test the PDO production ability of the recombinant strain, experiments were conducted in medium containing 2% (w/v) glucose or 2% (w/v) glycerol.…”

Section: Introducing Pdo Production Pathway To a Glycerol-producing Mmentioning

confidence: 99%

Recent developments in the microbial production of 1,3-propanediol

Tang

Liu³

et al. 2013

Biofuels

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1,3-propanediol (PDO) is a valuable chemical monomer to polymerize to polytrimethylene terephthalate with terephthalic acid. Polytrimethylene terephthalate is considered as one kind of prospective new polyester. Production of PDO by microbial fermentation leads to a significantly cost-effective process and decreases nonrenewable resources. This article summarizes the latest developments in the microbial production of PDO, including the selection of high-performance PDO-producing microorganisms, the conversion of crude glycerol derived from biodiesel plants to PDO and different fermentation strategies adopted in the fermentation process. Furthermore, genetic engineering is discussed, a technology that provides a more convenient method to obtain microorganisms with high PDO-producing ability. Microbial production of PDO would directly benefit the environment by reducing the use of fossil fuels and elevating the value of biodiesel production, which has become a hotspot in recent decades.

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“…Regarding native producers, mainly K. pneumoniae , increased 1,3-PDO production has been attempted by overexpression of genes directly involved in the 1,3-PDO biosynthesis pathway and inactivation of genes involved in byproduct formation. Regarding non-native producers, a 1,3-PDO biosynthesis pathway has been constructed in bacteria and yeasts that are not naturally able to produce 1,3-PDO by heterologous overexpression of genes from natural producers [10,15,16]. Among the engineered strains, 1,3-PDO production from glycerol using K. pneumoniae and E. coli strains looks the most promising, as shown by the high 1,3-PDO titers obtained (Table 1).…”

Section: Introductionmentioning

confidence: 99%

Biodiesel biorefinery: opportunities and challenges for microbial production of fuels and chemicals from glycerol waste

Almeida

Fávaro

Quirino

2012

Biotechnol Biofuels

201

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The considerable increase in biodiesel production worldwide in the last 5 years resulted in a stoichiometric increased coproduction of crude glycerol. As an excess of crude glycerol has been produced, its value on market was reduced and it is becoming a “waste-stream” instead of a valuable “coproduct”. The development of biorefineries, i.e. production of chemicals and power integrated with conversion processes of biomass into biofuels, has been singled out as a way to achieve economically viable production chains, valorize residues and coproducts, and reduce industrial waste disposal. In this sense, several alternatives aimed at the use of crude glycerol to produce fuels and chemicals by microbial fermentation have been evaluated. This review summarizes different strategies employed to produce biofuels and chemicals (1,3-propanediol, 2,3-butanediol, ethanol, n-butanol, organic acids, polyols and others) by microbial fermentation of glycerol. Initially, the industrial use of each chemical is briefly presented; then we systematically summarize and discuss the different strategies to produce each chemical, including selection and genetic engineering of producers, and optimization of process conditions to improve yield and productivity. Finally, the impact of the developments obtained until now are placed in perspective and opportunities and challenges for using crude glycerol to the development of biodiesel-based biorefineries are considered. In conclusion, the microbial fermentation of glycerol represents a remarkable alternative to add value to the biodiesel production chain helping the development of biorefineries, which will allow this biofuel to be more competitive.

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1,3-Propandiol production by engineered Hansenula polymorpha expressing dha genes from Klebsiella pneumoniae

Cited by 14 publications

References 18 publications

Enhance 1,3-propanediol production from crude glycerol in batch and fed-batch fermentation with two-phase pH-controlled strategy

Enhance 1,3-propanediol production from crude glycerol in batch and fed-batch fermentation with two-phase pH-controlled strategy

Recent developments in the microbial production of 1,3-propanediol

Biodiesel biorefinery: opportunities and challenges for microbial production of fuels and chemicals from glycerol waste

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