1-2B7B: Monoclonal antibody reacting to the 120 kDa polypeptide component of human epidermal hemidesmosomes

Abstract

Immunohistochemical and immunochemical analyses were performed on a monoclonal antibody designated 1-2B7B which was derived from immunizing mice with human prostate epithelial tissue. The 1-2B7B antigen was expressed not only along the acinous basement membrane zone (BMZ) of the prostate and testis, but also along the BMZ of the epithelia of several other organs including the skin, oesophagus, urinary bladder, ureter, stomach, intestine and bile duct. The antigenic epitope was not expressed in these tissues of lower mammals. Immunoelectron microscopic studies on normal human skin revealed that the 1-2B7B antigen was localized mainly just beneath the hemidesmosomes of basal keratinocytes, but not beneath melanocytes. Indirect immunofluorescence and immunoelectron microscopic studies on 1 M NaCl-split skin confirmed that this antigen was not separated from the cytoplasmic membrane of basal cells after salt treatment. SDS polyacrylamide gel electrophoresis of immunochemically purified protein from the epidermis demonstrated the molecular weight of the antigen to be 120 kDa. 1-2B7B monoclonal antibody should be a useful probe for studying the pathomechanism of some blistering diseases, as well as the assembly and function of the epidermal-dermal junction.

Anchoring Junctions As Drug Targets: Role in Contraceptive Development

“…Moreover, ␣6␤1 integrin does not seem to localize to the intermediate filament-based hemidesmosome in the testis but apparently to the actin-based basal ectoplasmic specialization (Chapin et al, 2001;Mulholland et al, 2001). In addition, except for a single report published nearly 2 decades ago that described the presence of an uncharacterized 120-kDa protein in the testis referred to as 1-2B7B (Zhang et al, 1991), there has been no advancement in the biology of the hemidesmosome in this organ. However, it is especially important that future studies address the possibility of cross-talk between hemidesmosomes and the blood-testis barrier, given their physical proximity.…”

Extensive Variant of Cutaneous Amyloidosis: Report of a Case with Electron-Microscopic and Immunohistochemical Studies of the Basement Membrane Zone at Sites of Amyloid Production

“…Naperville. 111.. USA) containing8% glycerin and 16% sucrose and frozen with acetone-carbon dioxide as described pre viously [ 13]. Double-immunofluorescence microscopy was performed using four monoclonal antibodies against BMZ components and a conventional antibody against amyloid P component.…”

“…The ultrastructural localization of BMZ components was deter mined by the avidin-biotin-peroxidase complex method with immunoelcctron microscopy [13], In brief, 6-pm serial cryosections were incubated with anti-type-IV. LDA-I.…”

A Case of Pretibial Dystrophic Epidermolysis Bullosa: Decreased Expression of the Non‐helical Domain of Type VII Collagen Molecule

“…After PBS washes, sections for immunoelectron microscopy were treated with 1% glutaraldehyde solution in PBS for 5 min and then serially incubated with 0.2 mg/ml diaminobenzidine (DAB) and a mixture ofDAB and 0.005% hydrogen peroxide. These sections were then postfixed with 1% osmium tetroxide, dehydrated with a graded alcohol series, and embedded in Spurr's resin, as previously described (13). Ultrathin sections were counterstained with Reynold's lead citrate and examined under an H500 electron microscope (Hitachi, Tokyo, Japan).…”