1,25-Dihydroxyvitamin D3 up-regulates TLR10 while down-regulating TLR2, 4, and 5 in human monocyte THP-1

Verma, Rewa; Jung, Jae-Chul; Kim, Jae Young

doi:10.1016/j.jsbmb.2013.12.012

Cited by 41 publications

(37 citation statements)

References 38 publications

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“…When cells were treated with 100 n M 1,25(OH) 2 D 3 , surface TLR10 expression was significantly enhanced at 24 h, reaching approximately 1.6 times the control level at 36 h and decreased gradually thereafter. Collectively, these results indicate that the surface TLR10 expression on HMO6 cells is upregulated by 1,25(OH) 2 D 3 , similarly to the results in our previous study with human THP-1 monocytes [10]. …”

Section: Resultssupporting

confidence: 89%

“…Thota et al [21] reported that 1,25(OH) 2 D 3 inhibits the lipopolysaccharide (LPS)-induced expression of proinflammatory cytokines, TLR4, and TLR5 in the myometrial smooth muscle, while enhancing IL-10 and TLR10 expression, suggesting that 1,25(OH) 2 D 3 and TLR10 may play inhibitory roles in infection-related inflammation. Similarly, our previous study revealed that TLR10 expression on human monocytes increased in response to treatment with 1,25(OH) 2 D 3 , suggesting possible regulatory roles of 1,25(OH) 2 D 3 and TLR10 in human monocytes [10]. …”

Section: Introductionmentioning

confidence: 63%

“…Our previous study revealed that TLR10 expression on human THP-1 monocytes is markedly enhanced by 1,25(OH) 2 D 3 , suggesting a possible regulatory role of TLR10 in human monocytes [10]. In order to test whether a similar result can be observed with microglia, the resident macrophages in the CNS, we used the immortalized human microglial cell line HMO6, which has previously been established and characterized to express microglia-macrophage lineage-specific antigens and to exert phagocytic activity [22].…”

Section: Resultsmentioning

confidence: 99%

“…It has been shown to enhance the expression of cathelicidin [2,3], CD14 [4], defensin β 2 [5], defensin β 4 [3] and NOD2 (nucleotide-binding oligomerization domain-containing protein 2) [6]. Simultaneously, 1,25(OH) 2 D 3 suppresses the expression of proinflammatory cytokines, MHC class II, CD40 [7,8] and Toll-like receptors (TLRs) on human monocytes [9,10], as well as their immune responses to TLR ligands [11,12,13,14]. …”

Section: Introductionmentioning

confidence: 99%

“…Previous studies suggest that TLR10 may function differently from other TLRs [10,20,21]. Thota et al [21] reported that 1,25(OH) 2 D 3 inhibits the lipopolysaccharide (LPS)-induced expression of proinflammatory cytokines, TLR4, and TLR5 in the myometrial smooth muscle, while enhancing IL-10 and TLR10 expression, suggesting that 1,25(OH) 2 D 3 and TLR10 may play inhibitory roles in infection-related inflammation.…”

Section: Introductionmentioning

confidence: 99%

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1,25-Dihydroxyvitamin D<sub>3</sub> Facilitates M2 Polarization and Upregulates TLR10 Expression on Human Microglial Cells

Verma

Kim²

2016

Neuroimmunomodulation

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Objective: To explore the effects of 1,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃], the active metabolite of vitamin D, on M1/M2 polarization of human microglia and the expression of Toll-like receptor 10 (TLR10) on these cells, which has been suggested to play an inhibitory role in inflammation previously. Methods: Microglial HMO6 cells were treated with 1,25(OH)₂D₃, and mRNA or protein levels of M1 and M2 cytokines and TLR10 were examined. Results: 1,25(OH)₂D₃ upregulated TLR10 in HMO6 cells at both mRNA and protein level. 1,25(OH)₂D₃ enhanced basal mRNA expression of M2 cytokines, such as IL-10 and CCL17, but did not affect the expression of M1 cytokines, including IL-12 and TNF-α. 1,25(OH)₂D₃ downregulated the lipopolysaccharide (LPS)-induced mRNA expression of M1 cytokines IL-12 and TNF-α. Concomitantly, it upregulated not only the M2 cytokines IL-10 and CCL17, but also TLR10 in microglial cells treated with LPS, in a concentration-dependent manner. Conclusions: Our results suggest that 1,25(OH)₂D₃ may exert anti-inflammatory action by facilitating the M2 polarization of human microglial cells.

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Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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1,25-Dihydroxyvitamin D<sub>3</sub> Facilitates M2 Polarization and Upregulates TLR10 Expression on Human Microglial Cells

Verma

Kim²

2016

Neuroimmunomodulation

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Vitamin D₃Metabolites Enhance the NLRP3-Dependent Secretion of IL-1β From Human THP-1 Monocytic Cells

et al. 2015

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Vitamin D3 has emerged as an important regulator of the immune system. With metabolic enzymes for vitamin D3 activation and vitamin D receptors (VDR) now identified in a variety of immune cells, the active vitamin D3 metabolite 1,25(OH)2D3, is thought to possess immunomodulatory properties. We examined whether 1,25(OH)2D3 might also enhance the NLRP3-dependent release of mature IL-1β from macrophages. PMA-differentiated THP-1 cells were stimulated with vitamin D3 metabolites and assessed for CYP27, CYP24, NLRP3, ASC, pro-caspase-1 expression by western blot and real-time qPCR as well as inflammasome activation with pro-inflammatory cytokine IL-1β release measured by ELISA. Exposure to 1,25(OH)2D3 had no effect on the basal expression levels of VDR; however, CYP27A1 transcript was suppressed and CYP24A1 transcript was substantively elevated. Both 1,25(OH)2D3 - and 25(OH)D3 induced IL-1β release from THP-1 cells, and these effects were blocked with application of the caspase-1 inhibitor YVAD and the NLRP3 inhibitors glyburide and Bay 11-7082. Interestingly, 1,25 (OH)2D3 exposure reduced NLRP3 protein expression but had no effect on ASC or pro-caspase-1 protein levels. The increase in mature IL-1β elicited by 1,25(OH)2D3 was modest compared to that found for ATP or C. difficile toxins. However, co-treatment of THP-1 cells with ATP and 1,25(OH)2D3 resulted in more IL-1β secretion than ATP or 1,25(OH)2D3 alone.

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TLR10 and Its Role in Immunity

Fore

Budipranama

Destiawan

2021

Handbook of Experimental Pharmacology

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1,25-Dihydroxyvitamin D3 up-regulates TLR10 while down-regulating TLR2, 4, and 5 in human monocyte THP-1

Cited by 41 publications

References 38 publications

1,25-Dihydroxyvitamin D<sub>3</sub> Facilitates M2 Polarization and Upregulates TLR10 Expression on Human Microglial Cells

1,25-Dihydroxyvitamin D<sub>3</sub> Facilitates M2 Polarization and Upregulates TLR10 Expression on Human Microglial Cells

Vitamin D₃Metabolites Enhance the NLRP3-Dependent Secretion of IL-1β From Human THP-1 Monocytic Cells

TLR10 and Its Role in Immunity

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1,25-Dihydroxyvitamin D3 up-regulates TLR10 while down-regulating TLR2, 4, and 5 in human monocyte THP-1

Cited by 41 publications

References 38 publications

1,25-Dihydroxyvitamin D<sub>3</sub> Facilitates M2 Polarization and Upregulates TLR10 Expression on Human Microglial Cells

1,25-Dihydroxyvitamin D<sub>3</sub> Facilitates M2 Polarization and Upregulates TLR10 Expression on Human Microglial Cells

Vitamin D3Metabolites Enhance the NLRP3-Dependent Secretion of IL-1β From Human THP-1 Monocytic Cells

TLR10 and Its Role in Immunity

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Product

Resources

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Vitamin D₃Metabolites Enhance the NLRP3-Dependent Secretion of IL-1β From Human THP-1 Monocytic Cells