1,25-Dihydroxyvitamin D3 increases Epidermal Growth Factor Receptor gene expression in BT-20 breast carcinoma cells

Desprez, Pierre-Yves; Poujol, Dominique; Falette, Nicole; Lefebvre, M. C.; Saez, S.

doi:10.1016/0006-291x(91)90880-g

Cited by 28 publications

(11 citation statements)

References 19 publications

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“…The cells of the basal layer and of the keratinizing layer may respond differently to the action of these factors due to changes for example in receptor number or affinity toward ligand. Vitamin D3 has been reported to increase the expression of EGF-receptors in a bone-derived cell line (Petkovich et al, 1987) and in breast carcinoma cells (Desprez et al, 1991) in association with increased binding of EGF. Endogenous production in keratinocytes of TGFa, which binds to EGF-receptor, seems to be involved in maintaining the balance between growth and differentiation.…”

Section: Discussionmentioning

confidence: 96%

Vitamin D₃and Calcipotriol Enhance the Secretion of Transforming Growth Factor-β1 and -β2 in Cultured Murine Keratinocytes

Koli

Keski‐Oja

1993

Growth Factors

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Vitamin D3 and its analogue calcipotriol (MC 903) inhibit the proliferation of cultured keratinocytes and induce their differentiation. Since TGF beta s are very potent inhibitors of keratinocyte growth we studied the effects of vitamin D3 and calcipotriol on the secretion of TGF beta in cultured murine keratinocytes. Vitamin D3 and calcipotriol (10(-6)-10(-9) M) inhibited the DNA-synthesis of mouse keratinocytes by 50-80% in a time and dose-dependent manner as measured by [3H]-thymidine incorporation. Analysis of the conditioned medium of the keratinocytes indicated that the cells secreted into their medium activity that inhibited the growth of indicator Mv1Lu mink lung epithelial cells. Neutralizing antibodies against TGF beta 1 and TGF beta 2 decreased, and when used together, prevented the observed growth inhibition of the indicator cells. Heat treatment of the conditioned medium, which activates latent forms of TGF beta, revealed higher levels of growth inhibitory activity in the medium from vitamin D3 and calcipotriol treated than from control cultures indicating that a fraction of TGF beta was in a latent form. Active TGF beta was, however, detected considerably more in vitamin D3 and calcipotriol treated cultures than in control cultures. Immunoblotting analysis of the medium revealed enhanced secretion of TGF beta protein. These results indicate that enhanced TGF beta 1 and TGF beta 2 secretion and activity is associated with vitamin D3-mediated growth inhibition of cultured keratinocytes.

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Section: Discussionmentioning

confidence: 96%

Vitamin D₃and Calcipotriol Enhance the Secretion of Transforming Growth Factor-β1 and -β2 in Cultured Murine Keratinocytes

Koli

Keski‐Oja

1993

Growth Factors

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“…This is consistent with a previous observation by Desprez et al . in breast cancer (33). Interestingly, reduction in EGFR expression has also been reported in breast cancer cell lines (34).…”

Section: Discussionmentioning

confidence: 99%

Impact of Short-term 1,25-Dihydroxyvitamin D3 on the Chemopreventive Efficacy of Erlotinib against Oral Cancer

Bothwell

Shaurova

Merzianu³

et al. 2015

Cancer Prevention Research

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Activation of the epidermal growth factor receptor (EGFR) pathway is an early event in head and neck carcinogenesis. As a result, targeting EGFR for chemoprevention of head and neck squamous cell carcinomas (HNSCC) has received considerable attention. In the present study, we examined the impact of 1,25(OH)2D3, the active metabolite of the nutritional supplement vitamin D on the chemopreventive efficacy of the EGFR inhibitor, erlotinib, against HNSCC. Experimental studies were conducted in patient derived xenografts (PDX) and the 4-nitroquinoline-1-oxide (4NQO) carcinogen-induced model of HNSCC. Short-term treatment (4 weeks) of PDX-bearing mice with 1,25(OH)2D3 and erlotinib resulted in significant inhibition of tumor growth. Non-invasive magnetic resonance imaging (MRI) enabled longitudinal monitoring of disease progression in the 4NQO model with 100% of control animals showing evidence of neoplastic lesions by 24 weeks. Among the experimental groups, animals treated with the combination regimen showed the greatest reduction in tumor incidence and volume (p<0.05). Combination treatment was well tolerated and was not associated with any significant change in body weight. Histopathologic assessment revealed a significant reduction in the degree of dysplasia with combination treatment. Immunoblot analysis of whole tongue extracts showed down regulation of phospho-EGFR and phospho-Akt with the combination regimen. These results highlight the potential of 1,25(OH)2D3 to augment the efficacy of erlotinib against HNSCC. Further optimization of schedule and sequence of this combination regimen along with investigation into the activity of less calcemic analogues or dietary vitamin D is essential to fully realize the potential of this approach.

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“…Bareis et al., 57 however, demonstrated that the interaction between EGF and vitamin D is quite complex, as EGF treatment increased VDR expression in highly differentiated, slowly dividing colon cancer cell lines, but decreased expression in poorly differentiated, highly proliferative lines. Variable effects of 1,25‐dihydroxyvitamin D on EGFR have also been reported in different studies of breast cancer cell lines, with some finding that calcitriol decreased EGFR whereas others showed increased EGFR levels 36,58,59 . Calcitriol has been reported to alter intracellular EGF processing in BT‐20 breast cancer cells 60 …”

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confidence: 90%

Vitamin D's role in cell proliferation and differentiation

2008

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Vitamin D has pleiotropic effects that go beyond its traditional role in calcium homeostasis. Hundreds of genes with vitamin D receptor response elements directly or indirectly influence cell cycling and proliferation, differentiation, and apoptosis. Vitamin D compounds also have effects on cell function that are nongenomic. The noncalcemic actions of vitamin D influence normal and pathological cell growth, carcinogenesis, immune function, and cardiovascular physiology. This review examines many of the various mechanisms by which vitamin D alters cellular growth and differentiation and explores cell-specific factors that influence responsiveness to vitamin D.

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1,25-Dihydroxyvitamin D3 increases Epidermal Growth Factor Receptor gene expression in BT-20 breast carcinoma cells

Cited by 28 publications

References 19 publications

Vitamin D₃and Calcipotriol Enhance the Secretion of Transforming Growth Factor-β1 and -β2 in Cultured Murine Keratinocytes

Vitamin D₃and Calcipotriol Enhance the Secretion of Transforming Growth Factor-β1 and -β2 in Cultured Murine Keratinocytes

Impact of Short-term 1,25-Dihydroxyvitamin D3 on the Chemopreventive Efficacy of Erlotinib against Oral Cancer

Vitamin D's role in cell proliferation and differentiation

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1,25-Dihydroxyvitamin D3 increases Epidermal Growth Factor Receptor gene expression in BT-20 breast carcinoma cells

Cited by 28 publications

References 19 publications

Vitamin D3and Calcipotriol Enhance the Secretion of Transforming Growth Factor-β1 and -β2 in Cultured Murine Keratinocytes

Vitamin D3and Calcipotriol Enhance the Secretion of Transforming Growth Factor-β1 and -β2 in Cultured Murine Keratinocytes

Impact of Short-term 1,25-Dihydroxyvitamin D3 on the Chemopreventive Efficacy of Erlotinib against Oral Cancer

Vitamin D's role in cell proliferation and differentiation

Contact Info

Product

Resources

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Vitamin D₃and Calcipotriol Enhance the Secretion of Transforming Growth Factor-β1 and -β2 in Cultured Murine Keratinocytes

Vitamin D₃and Calcipotriol Enhance the Secretion of Transforming Growth Factor-β1 and -β2 in Cultured Murine Keratinocytes