1.2% Hydrogen gas inhalation protects the endothelial glycocalyx during hemorrhagic shock: a prospective laboratory study in rats

Sato, Tsunehisa; Mimuro, Soichiro; Katoh, Takasumi; Kurita, Tadayoshi; Truong, Sang Kien; Kobayashi, Kensuke; Makino, Hiroshi; Doi, Matsuyuki; Nakajima, Yoshiyuki

doi:10.1007/s00540-020-02737-3

Cited by 12 publications

(18 citation statements)

References 34 publications

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“…The glycocalyx is present on the surface of glomerular capillaries and podocytes in the kidney and has been shown to be exfoliated by exposure to lipopolysaccharides. Hydrogen is thought to protect the glycocalyx ( 49 ). There were decreased expression levels of syndecan-1 in the glycocalyx when hydrogen was inhaled in a non-traumatic hemorrhagic shock rat model, showing that hydrogen contributed to the prolongation of survival ( 49 ).…”

Section: Discussionmentioning

confidence: 99%

“…Hydrogen is thought to protect the glycocalyx ( 49 ). There were decreased expression levels of syndecan-1 in the glycocalyx when hydrogen was inhaled in a non-traumatic hemorrhagic shock rat model, showing that hydrogen contributed to the prolongation of survival ( 49 ). One study showed that an increase in syndecan-1 levels in tubular epithelial cells was associated with improved graft function and prolonged engraftment ( 50 ).…”

Section: Discussionmentioning

confidence: 99%

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Prevention of Chronic Rejection of Marginal Kidney Graft by Using a Hydrogen Gas-Containing Preservation Solution and Adequate Immunosuppression in a Miniature Pig Model

et al. 2021

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In clinical kidney transplantation, the marginal kidney donors are known to develop chronic allograft rejection more frequently than living kidney donors. In our previous study, we have reported that the hydrogen gas-containing organ preservation solution prevented the development of acute injuries in the kidney of the donor after cardiac death by using preclinical miniature pig model. In the present study, we verified the impact of hydrogen gas treatment in transplantation with the optimal immunosuppressive protocol based on human clinical setting by using the miniature pig model. Marginal kidney processed by hydrogen gas-containing preservation solution has been engrafted for long-term (longer than 100 days). A few cases showed chronic rejection reaction; however, most were found to be free of chronic rejection such as graft tissue fibrosis or renal vasculitis. We concluded that marginal kidney graft from donor after cardiac death is an acceptable model for chronic rejection and that if the transplantation is carried out using a strict immunosuppressive protocol, chronic rejection may be alleviated even with the marginal kidney.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Prevention of Chronic Rejection of Marginal Kidney Graft by Using a Hydrogen Gas-Containing Preservation Solution and Adequate Immunosuppression in a Miniature Pig Model

et al. 2021

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“…Three of the four agents targeting oxidative stress and apoptosis successfully protected the glycocalyx, as reflected by increased thickness of the glycocalyx (24) or reduced shedding of glycocalyx components (59). Addition of hydrogen (H 2 ) gas to mechanical ventilation was investigated by two independent research groups, both revealing a protective effect on the glycocalyx (57,59). Direct targeting of endothelial barrier with sphingosine-1-phosphate (S1P) increased thickness of the glycocalyx (46).…”

Section: Figmentioning

confidence: 99%

Therapeutically Targeting Microvascular Leakage in Experimental Hemorrhagic SHOCK: A Systematic Review and Meta-Analysis

Leeuwen

Borgdorff

Dekker

et al. 2021

Shock

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Background: Microvascular leakage is proposed as main contributor to disturbed microcirculatory perfusion following hemorrhagic shock and fluid resuscitation, leading to organ dysfunction and unfavorable outcome. Currently, no drugs areavailabletoreduceor preventmicrovascular leakageinclinicalpractice.Wethereforeaimedtoprovideanoverviewoftherapeutic agents targeting microvascular leakage following experimental hemorrhagic shock and fluid resuscitation. Methods: PubMed, EMBASE.com, and Cochrane Library were searched in January 2021 for preclinical studies of hemorrhagic shock using any therapeutic agent on top of standard fluid resuscitation. Primary outcome was vascular leakage, defined as edema, macromolecule extravasation, or glycocalyx degradation. Drugs were classified by targeting pathways and subgroup analyses were performed per organ. Results: Forty-five studies, published between 1973 and 2020, fulfilled eligibility criteria. The included studies tested 54 different therapeutics mainly in pulmonary and intestinal vascular beds. Most studies induced trauma besides hemorrhagic shock. Forty-four therapeutics (81%) were found effective to reduce microvascular leakage, edema formation, or glycocalyx degradation in at least one organ. Targeting oxidative stress and apoptosis was the predominantly effective strategy (SMD: À2.18, CI [À3.21, À1.16], P < 0.0001). Vasoactive agents were found noneffective in reducing microvascular leakage (SMD: À0.86, CI [À3.07, 1.36], P ¼ 0.45). Conclusion: Pharmacological modulation of pathways involved in cell metabolism, inflammation, endothelial barrier regulation, sex hormones and especially oxidative stress and apoptosis were effective in reducing microvascular leakage in experimental hemorrhagic shock with fluid resuscitation. Future studies should investigate whether targeting these pathways can restore microcirculatory perfusion and reduce organ injury following hemorrhagic shock. Systematic review registration number: CRD42018095432.

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“…Ethological observations have shown that the inclusion of HRW in the diet of Chinchilla lanigera increases the proportion of active behavior in the daily balance by an average of 24% [44]. Molecular hydrogen can be introduced in various ways: by inhalation of H2; with water enriched in molecular hydrogen; taking molecular hydrogen baths; using eye drops with molecular hydrogen [45][46][47][48][49]. The enrichment of the body with H2 increases the concentration of the hydrogen antioxidant in the blood [50].…”

Section: Introductionmentioning

confidence: 99%

The condition of the hairline of Chinchilla lanigera after the introduction of a hydrogen antioxidant into the diet

2021

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It was found that the inclusion of water enriched with molecular hydrogen into the diet of a long-tailed chinchilla changed the fur quality indicators. In animals of the experimental group, the guard and downy hairs were thinner than in the control group. The length of downy hair in the experimental group was higher, the number of hairs in the follicle in the chinchillas of the experimental group was greater than in the control group, besides, the hair was stronger and softer. The animals of the experimental group showed less tendency to gnaw out fur. When considering the data on the chemical composition, it was found that in the dry matter of the hair of the animals of the experimental group, there were less organic substances, and there were more minerals in comparison with the animals of the control group.

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1.2% Hydrogen gas inhalation protects the endothelial glycocalyx during hemorrhagic shock: a prospective laboratory study in rats

Cited by 12 publications

References 34 publications

Prevention of Chronic Rejection of Marginal Kidney Graft by Using a Hydrogen Gas-Containing Preservation Solution and Adequate Immunosuppression in a Miniature Pig Model

Prevention of Chronic Rejection of Marginal Kidney Graft by Using a Hydrogen Gas-Containing Preservation Solution and Adequate Immunosuppression in a Miniature Pig Model

Therapeutically Targeting Microvascular Leakage in Experimental Hemorrhagic SHOCK: A Systematic Review and Meta-Analysis

The condition of the hairline of Chinchilla lanigera after the introduction of a hydrogen antioxidant into the diet

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