1,2-Diarylethylenediamines as fluorogenic reagents for catecholamines

Umegae, Yoshihiko; Nohta, Hitoshi; Ohkura, Yosuke

doi:10.1016/s0003-2670(00)80736-9

Cited by 14 publications

(2 citation statements)

References 11 publications

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“…48 The DL-or meso-diarylethylenediamines (28 species), vicinal arylaliphatic diamino compounds that have been investigated for detecting fluorogenic reagents for catecholamines, all react with catecholamines under mild conditions (pH 6.5 -6.8, 37 -50° C) in the presence of potassium hexacyanoferrate(III) to produce fluorescence. 49 Of the compounds, 1,2-bis(3,4-dimethoxyphenyl)ethylenediamine, 1,2-bis(4-methoxyphenyl)ethylenediamine and 1,2-bis(4-ethoxyphenyl)ethylenediamine, all in meso form,, are most sensitive to all the catecholamines.…”

Section: Sialic Acids and Dehydroascorbic Acidmentioning

confidence: 98%

High Performance Liquid Chromatography of Bioactive Substances Using Bifunctional Fluorogenic Reagents for Derivatization A Review

Ohkura

1989

ANAL. SCI.

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Pre-and postco umn derivatization methods employing recently-developed Bifunctional fluorogenic reagents for high performance liquid chromatography have been demonstrated to be successful regarding both sensitivity and selectivity in the determination of bioactive substances and their related enzymes. The reagents were designed mostly based on the principle that vicinal or geminal, homo-or hetero-Bifunctional (diamino, amino-imino, aminosulfhydryl or amino-hydroxyl) compounds react with 1,2-dioxo compounds (1,2-ketols, glyoxals, 1,2-diketones or 1,2-quinones) or monooxo compounds (aldehydes) to yield fluorescent cyclic products. These reagents have been introduced into the quantification process of diverse bioactive substances in biological samples at the femtomole level.

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Section: Sialic Acids and Dehydroascorbic Acidmentioning

confidence: 98%

High Performance Liquid Chromatography of Bioactive Substances Using Bifunctional Fluorogenic Reagents for Derivatization A Review

Ohkura

1989

ANAL. SCI.

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“…Thus the fluorescence reaction was selective for catechol compounds. 61 Postcolumn derivatization methods are based on trihydroxyindole (THI)62.63 or OPA reactions. The postcolumn reactions require the use of a reactor, an extra pump, a y-piece, and a reaction coil placed between the column and the fluorometric detector.…”

Section: Fluorometric Detectionmentioning

confidence: 99%

Plasma Catecholamines: Laboratory Aspects

Nyyssönen

1989

Critical Reviews in Clinical Laboratory Sciences

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In this review the methods used for analysis of plasma catecholamines in clinical chemical laboratories are discussed. The physiology of catecholamines as well as their measuring indications are discussed, together with concise evaluation of the methods most commonly used, namely indirect radioenzymatic assays or direct determinations by high-performance liquid chromatography combined with either electrochemical or fluorometric detection. The main advantage of radioenzymatic assay is its sensitivity and thus the need for only a small sample. Liquid chromatographic methods in general are less tedious, relatively rapid, and cheap, and omit the use of radionuclides. Both of these methods, however, are subject to a number of analytical errors, which can only be avoided by proper development of methods and skilled use of these methods. Little routine work is done using either radioimmunoassay or gas-chromatography.

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Macdonald¹,

Lingard²

1993

Dictionary of Analytical Reagents

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1,2-Diarylethylenediamines as fluorogenic reagents for catecholamines

Cited by 14 publications

References 11 publications

High Performance Liquid Chromatography of Bioactive Substances Using Bifunctional Fluorogenic Reagents for Derivatization A Review

High Performance Liquid Chromatography of Bioactive Substances Using Bifunctional Fluorogenic Reagents for Derivatization A Review

Plasma Catecholamines: Laboratory Aspects

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