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Türe, Mustafa; Haliloğlu, H. İbrahim; Altuntas, Cetin; Boran, Halis; Kutlu, İlyas

doi:10.4194/1303-2712-v14_2_22

Cited by 15 publications

(5 citation statements)

References 8 publications

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“…Trimmomatic (v0.38) (Bolger et al, 2014) was used to remove adapter sequences and trim out bases of low quality. Genome assembly was done with the SPAdes pipeline (v3.15.4) (Nurk et al, 2017) against the reference genome sequence of L. garvieae ATCC 49156 (GenBank assembly accession: GCF_000269925.1) because 16S rRNA sequence and biochemical tests indicated that Lg-per strain was L. garvieae (Ture et al, 2014Ture & Altinok, 2016) and L. petauri RJAA.1 (GenBank assembly accession: GCF_014830225.1). The draft genome was polished using the Burrows-Wheeler Aligner (BWA; v0.7.17) tool (Li & Durbin, 2009) that uses the Burrows-Wheeler Transform (BWT) logarithm for aligning reads to reference sequences and QUAST (v5) to create N50 (Mikheenko et al, 2018).…”

Section: Me Thods and Materials Smentioning

confidence: 99%

“…During the early years of the lactococcosis outbreaks in Turkey, gram-positive, oxidase and catalase-negative, non-motile, nonspore-forming, bacteria were isolated from a lactococcosis outbreak in rainbow trout in Turkey (Ture et al, 2014). Based on investigation of the 16S rRNA gene sequence and biochemical characterization, the Lg-per strain was classified as L. garvieae .…”

Section: Introductionmentioning

confidence: 99%

“…Based on investigation of the 16S rRNA gene sequence and biochemical characterization, the Lg-per strain was classified as L. garvieae . A cumulative mortality rate of 98% was reported after an experimental intraperitoneal challenge with the Lg-per strain (Ture et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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NGS analysis revealed that Lactococcus garvieae Lg‐Per was Lactococcus petauri in Türkiye

Altınok

Öztürk

Türe

2022

Journal of Fish Diseases

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Lactococcus garvieae Lg-per was originally isolated from rainbow trout cultured in cages located on the Turkish coast of the Black Sea in 2011. A whole genome sequence of Lg-per was performed in the present study. The complete genome of Lg-per mapped to the reference genomes of L. garvieae (GCF_000269925.1) and Lactococcus petauri (GCF_014830225.1) had a total of 1,694,407 and 1,945,297 base pairs, respectively.Lg-per had 1955 protein-coding genes and 4 rRNA, 46 tRNA and 1 tmRNA operons.The orthoANI value was 98.30% between Lg-per and L. petauri (GCF_014830225.1) and 93.1% between Lg-per and L. garvieae (GCF_000269925.1). A phylogenetic tree generated from the whole genome sequences (WGS) of several Lactococcus species found that L. petauri (GCA 002154895) was closely related to the Lg-per strain with 98% similarity. Although L. garvieae Lg-per was confirmed as L. garvieae based on phenotypical, biochemical and 16S rRNA sequence, WGS of the Lg-per strain revealed that Lg-per was L. petauri. Using a 16S rRNA-based PCR detection approach, Lg-per was misdiagnosed as L. garvieae since its 16S rRNA gene was 99.9% similar to that of L. garvieae strains. Consequently, the 16S rRNA-based PCR detection approach may not be adequate for the identification of the Lactococcus genus. This is the first study to document the presence of L. petauri in Türkiye. L. garvieae isolates should be analysed using WGS since the same issue might occur in other countries.

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Section: Me Thods and Materials Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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NGS analysis revealed that Lactococcus garvieae Lg‐Per was Lactococcus petauri in Türkiye

Altınok

Öztürk

Türe

2022

Journal of Fish Diseases

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“…Polysaccharide capsule is the major virulence factor in L. garvieae infection [29]. The capsulated strains resist to phagocytosis, but some non-capsulated strains are pathogenic in fish causing high mortality in rainbow trout [34], thus, the bacterial capsule may not the sole determinant of the bacterial pathogenicity. Haemolytic toxin is known to cause mortality in fish via intramuscular injection and an intracellular toxin with a low leukocidal activity reported by the bacterial isolates recovered from the diseased fish [35].…”

Section: Diseases Caused By Lactococcus Members In Aquaculture 21 Disease Caused By L Garvieaementioning

confidence: 99%

Lactococcosis a Re-Emerging Disease in Aquaculture: Disease Significant and Phytotherapy

Soltani

Baldisserotto

Shekarabi

et al. 2021

Veterinary Sciences

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Lactococcosis, particularly that caused by Lactococcus garvieae, is a major re-emerging bacterial disease seriously affecting the sustainability of aquaculture industry. Medicinal herbs and plants do not have very much in vitro antagonism and in vivo disease resistance towards lactococcosis agents in aquaculture. Most in vitro studies with herbal extractives were performed against L. garvieae with no strong antibacterial activity, but essential oils, especially those that contain thymol or carvacrol, are more effective. The differences exhibited by the bacteriostatic and bactericidal functions for a specific extractive in different studies could be due to different bacterial strains or parts of chemotypes of the same plant. Despite essential oils being shown to have the best anti-L. garvieae activity in in vitro assays, the in vivo bioassays required further study. The extracts tested under in vivo conditions presented moderate efficacy, causing a decrease in mortality in infected animals, probably because they improved immune parameters before challenging tests. This review addressed the efficacy of medicinal herbs to lactococcosis and discussed the presented gaps.

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“…Analyses indicate that the capsular gene cluster is a genomic island, because of the presence of insertion sequences (ISs) on both ends of the capsular gene cluster. Even though the polysaccharide capsule is widely regarded as a major virulence factor of L. garvieae, it has been shown that non-capsulated strains Lgper and ATCC ® 49156 are pathogenic to rainbow trout, causing 89% and 98% mortality, respectively (Türe et al 2014). These results suggest that the presence of the polysaccharide capsule cannot be directly correlated with pathogenicity in fish.…”

Section: Introductionmentioning

confidence: 99%

Detection of virulence factors of South African Lactococcus garvieae isolated from rainbow trout, Oncorhynchus mykiss (Walbaum)

Meyburgh

Bragg

Boucher

2018

Onderstepoort Journal of Veterinary Research

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Lactococcus garvieae is a Gram-positive bacterium that causes mortalities in freshwater and marine fish worldwide and therefore results in severe economic losses in the aquaculture industry. Apart from the apparent integral role of the exopolysaccharide (EPS) capsule in pathogenesis, factors associated with virulence of this bacterium are poorly understood. However, recent studies have indicated that the ability of L. garvieae to cause disease does not depend on the presence of the EPS capsule. Lack of knowledge of virulence factors, pathogenesis and serology of L. garvieae is an impediment to the development of effective typing methods and control measures. This study, therefore, aimed to detect the presence of EPS capsules and other putative virulence factors in South African L. garvieae fish pathogenic isolates and a non-virulent isolate, and to identify possible candidates for subunit vaccine development. No indication of the presence of the EPS capsule was detected by negative staining or amplification of the EPS biosynthesis gene cluster in the virulent isolates or the avirulent strain, discrediting the notion that the EPS capsule is the sole determinant of virulence. However, a set of putative virulence factor genes was detected in all isolates, and candidates for subunit vaccine development (enolase, lactate dehydrogenase phosphoenolpyruvate-protein phosphotransferase) were identified by identification of extracellular proteins of virulent strains.

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Cited by 15 publications

References 8 publications

NGS analysis revealed that Lactococcus garvieae Lg‐Per was Lactococcus petauri in Türkiye

NGS analysis revealed that Lactococcus garvieae Lg‐Per was Lactococcus petauri in Türkiye

Lactococcosis a Re-Emerging Disease in Aquaculture: Disease Significant and Phytotherapy

Detection of virulence factors of South African Lactococcus garvieae isolated from rainbow trout, Oncorhynchus mykiss (Walbaum)

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