Abstract:Aim.To determine the expression profiles of a set of cancer-associated genes in prostate tumors, using various normalization protocols (with 1, 2 and 4 reference genes) and to optimize a combination of reference genes to calculate the relative expression (RE) of the investigated genes in prostate cancers. Methods. Relative expression level of 23 genes was analyzed by quantitative PCR (qPCR) in 37 prostate cancer tissues (T) with different Gleason scores (GL) and at various stages and compared with 37 correspon… Show more
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