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Abstract

The purpose of this work was to investigate whether a probiotic bacterium, Lactobacillus plantarum 299v, could affect Escherichia coli-induced passage of mannitol across the intestinal wall. Sprague-Dawley rats were pretreated for one week by either tube feeding with L. plantarum 299v twice daily, free access to L. plantarum 299v by adding the bacterium in the drinking water, or negative control receiving regular feeding. Intestinal segments were mounted in Ussing chambers and the mucosa was exposed to control medium, E. coli, and L. plantarum 299v (alone or together). [14C]Mannitol was added as a marker of intestinal permeability and samples were taken from the serosal side. E. coli exposure induced a 53% increase in mannitol passage across the intestinal wall (P < 0.05). One week of pretreatment with L. plantarum 299v in the drinking water abolished the E. coli-induced increase in permeability. Tube feeding for one week or short-term addition of L. plantarum 299v in the Ussing chambers had no effect on the permeability provoked by E. coli challenge. Notably, L. plantanum 299v itself did not change the intestinal passage of mannitol. These data demonstrate that pretreatment with L. plantarum 299v, which is a probiotic bacterium, protects against E. coli-induced increase in intestinal permeability, and that L. plantarum 299v alone has no influence on the intestinal permeability. Thus, this study supports the concept that probiotics may exert beneficial effects in the gastrointestinal tract.

Effect of Lactobacillus acidophilus KFRI342 on the development of chemically induced precancerous growths in the rat colon

“…acidophilus KFRI342 reduced the colonization of E. coli and aerobic bacteria (TPC) in the colon, perhaps by colonizing the gut itself, and reduced the pH in the gastrointestinal tract. Lactobacillus plantarum 299v reduced E. coli-induced injury and intestinal permeability in rats (Mangell et al, 2002). A culture condensate of Bifidobacterium longum inhibited E. coli translocation from the gastrointestinal tract in antibiotic-decontaminated specificpathogen-free and germ-free mice (Suzuki et al, 1997).…”

Brain?Gut?Microbe Communication in Health and Disease

“…Pretreatment with a probiotic mixture containing L. helveticus and L. rhamnosus was shown to prevent bacterial translocation and improve intestinal barrier function in rats following chronic psychological stress (Zareie et al, 2006). Similarly, L. Plantarum 299V was shown to inhibit Escherichia coli -induced intestinal permeability (Mangell et al, 2002). The involvement of the vagus nerve is also considered likely in the light of recent studies (Bravo et al, 2011; Cryan and O’Mahony, 2011).…”

The effects of Lactobacillus plantarum on small intestinal barrier function and mucosal gene transcription; a randomized double-blind placebo controlled trial

“…Up to now, no therapeutic agents have been developed that are able to successfully restore the intestinal mucosal barrier and thereby influence disease outcome16. Positive effects on intestinal barrier function have been attributed to probiotic bacteria17181920212223. However, further research is needed to prove these beneficial effects in humans and to gain further insight into the mechanisms through which live bacterial organisms improve the human gut barrier function2425.…”