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Gopko, A. V.; Zakhidov, S. T.; Маршак, Т. Л.; Kulibin, A. Yu.; Semenova, M. L.; Makarov, Alexander А.

doi:10.1023/a:1026156728252

Doklady Biological Sciences

2003

DOI: 10.1023/a:1026156728252

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A. V. Gopko

S. T. Zakhidov

Т. Л. Маршак

et al.

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Cited by 3 publications

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“…We previously suggested that the high level of genetic instability accounts for the fact that a small group of mice of the SAMP1 strain has acquired a new developmental algorithm that might lead to the elimination of accelerated aging [1]. Another hypothesis explains the sharp increase in the average life span in SAMP1 mice from 9.5 to 18-20 months by changes in the microbiological status and elimination of the pathogenic milieu, rather than by genetic factors [13].…”

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Spermatogenic Structure in the Long-Lived SAMP1 Mice Prone to Accelerated Aging

et al. 2005

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We previously described the cytogenetic features of spermatogenesis in SAMP1 mice prone to accelerated aging, which have for the first time overcome the maximum life span limit, 15 months [1]. In 18-to 28-month-old SAMP1 mice, sex cells develop against the background of a high genetic instability, namely, the extremely increased frequency of spontaneous chromosomal mutations and nuclear abnormalities. The main goal of this study was quantitative and morphological analyses of spermatogenic epithelium in these unique animals.Male SAMP1 mice aged 18-28 months were used in this study. They were also compared with young (twoto three-month-old) mice and those of the critical age (14-15-months). All animals were kept under standard vivarium conditions and received water and food ad libitum.After fixation in an acetic acid-glycerol mixture, one testis of each animal was disrupted mechanically, and the homogenate was suspended. Various spermatogenic cells were counted using a hemocytometer and a phase-contrast device (Opton, Germany) at a magnification of × 400. The quantitative data were treated statistically using the STATISTICA software package. Significant differences between mean values were determined using Strudent's t test with the standard 5% significance level.The other testis of each animal was divided in half before fixation in Bouen's solution. Sections 7 µ m in thickness were stained with hematoxylin-eosin, and their histological analysis was made. Figure 1 shows the ultimate results of spermatogenic epithelium cell count in SAMP1 mice prone to accelerated aging. It can be seen that, in the gonads of 18-to 28-month-old males, the number of spermatogonia (stem cells + those undergoing differentiation), as well as the number of pachytene spermatocytes, round spermatids, and spermia, was significantly lower than in two-to threemonth-old and 14-to 15-month-old males. The differences were statistically significant at p < 0.05. Nevertheless, note that the spermatogonium compartment is still numerically stable in 14-to 15-month-old animals, and aging of the latter leads to changes in the meiotic phase of spermatogenesis, which is seen from a significant decrease in the number of pachytene spermatocytes, spermatids, and spermia.Our calculations showed that the population density of somatic multifunctional Sertoli cells integrated into the system of spermatogenesis did not decrease with time.The quantitative analysis leads to the following conclusions. The number of spermatogenic cells changes with age (from 2-3 to 14-15 months) in a way that is in strict conformity with the concept suggesting that the number of these cells decreases with increasing sper- CELL BIOLOGY

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Spermatogenic Structure in the Long-Lived SAMP1 Mice Prone to Accelerated Aging

et al. 2005

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Spermatogenesis pattern in SAMP1 mice prone to accelerated senescence: An experimental study

et al. 2006

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Cysteine as a possible protective agent against damaging action of ionizing radiation on spermatogenic epithelium

Mamina¹

2018

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Цель исследования-сравнительная оценка морфологических изменений структурных компонентов семенника и сперматозоидов у мышей линии BALB/c после облучения в дозе 1 грэй (Гр) с предварительным введением цистеина и без его применения. Материал и методы. Эксперименты проводили на половозрелых самцах линии BALB/c (n=250). Животных разделили на три группы: 1-я группа-контрольная, в нее включены мыши, которых не подвергали облучению; 2-я группа-мыши, получившие облучение в дозе 1 Гр; 3-я группа-мыши, получившие цистеин в дозе 900 мг/кг внутрибрюшинно и (через 15-20 мин) облучение в дозе 1 Гр. Вивисекцию животных производили путем дислокации шейных позвонков на 4, 8, 16, 24, 48-е сутки после облучения. Оценивали динамику морфологических изменений структурных компонентов семенников и сперматозоидов. Морфологические исследования семенников проводили на микропрепаратах, окрашенных гематоксилином и эозином по Майеру, количественный анализ сперматогенных клеток и эндокриноцитов-в суспензии семенника. Подсчет сперматозоидов проводили с помощью камеры Горяева, для морфологического исследования использовали мазки из эпидидимальных сперматозоидов Результаты. При морфологическом анализе семенников выявлены деструктивные изменения в семенных канальцах мышей 2-й и 3-й групп, приводящие к снижению числа сперматогенных клеток и сперматозоидов (p<0,05). Цитологический анализ показал увеличение патологических форм сперматозоидов. Введение цистеина способствовало поддержанию на более высоком уровне количества сперматогенных клеток и сперматозоидов, а также увеличению числа жизнеспособных сперматозоидов у мышей 3-й группы. Выводы. Облучение в дозе 1 Гр вызывает патоморфологические изменения в структурных компонентах семенника, снижение числа сперматогенных клеток и сперматозоидов, увеличение патологических форм сперматозоидов. Введенный до облучения цистеин оказал протективное действие, о чем свидетельствовали количественные показатели сперматогенных клеток, сперматозоидов и характеристика их морфофункционального состояния.

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Cited by 3 publications

References 4 publications

Spermatogenic Structure in the Long-Lived SAMP1 Mice Prone to Accelerated Aging

Spermatogenic Structure in the Long-Lived SAMP1 Mice Prone to Accelerated Aging

Spermatogenesis pattern in SAMP1 mice prone to accelerated senescence: An experimental study

Cysteine as a possible protective agent against damaging action of ionizing radiation on spermatogenic epithelium

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