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Papaceit, Montserrat; Juan, Elvira

doi:10.1023/a:1009218508672

Cited by 21 publications

(10 citation statements)

References 18 publications

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“…This finding means that chromosome 4 is the only haplosufficient autosome and is also by far the longest region of haplosufficiency among autosomes in D. melanogaster. In this respect, it is interesting to note that in the only species tested within the genus Drosophila where POF is not detected, D. willistoni, the F element is fused to one of the autosomes, element E corresponding to 3R in D. melanogaster (32). We speculate that the potential POF-supported regulatory mechanism is not needed when the F element is part of one of the major autosomes.…”

Section: Discussionmentioning

confidence: 89%

“…We did not see any signal in Drosophila willistoni either. It is interesting to note that D. willistoni is the only tested species that has the F element fused to one of the major autosomes (32). It is also the only Drosophila where we fail to detect POF with any of the four used antibodies.…”

Section: Male X-specific Binding Is Conserved In Evolution Our Earlimentioning

confidence: 87%

“…There was no signal in Scaptodrosophila lebanonensis, which is the form farthest away from D. melanogaster. We tested S. lebanonensis because it has been suggested to have the F element fused to the X chromosome in the fashion of D. busckii (32). We did not see any signal in Drosophila willistoni either.…”

Section: Male X-specific Binding Is Conserved In Evolution Our Earlimentioning

confidence: 94%

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Painting of fourth in genus Drosophila suggests autosome-specific gene regulation

Larsson

Svensson

Stenberg

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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Painting of fourth (POF) is a chromosome-specific protein in Drosophila and represents the first example of an autosome-specific protein. POF binds to chromosome 4 in Drosophila melanogaster, initiating at the proximal region, followed by a spreading dependent on chromosome 4-specific sequences or structures. Chromosome-specific gene regulation is known thus far only as a mechanism to equalize the transcriptional activity of the single male X chromosome with that of the two female X chromosomes. In Drosophila, a complex including the male-specific lethal proteins, ''paints'' the male X chromosome, mediating its hypertranscription, explained to some extent by the acetylation of lysine 16 on histone H4. Here, we show that Pof is essential for viability in both sexes and for female fertility. POF binding to an autosome, the F element, is conserved in genus Drosophila, indicating functional conservation of the autosome specificity. In three of nine studied species, POF binds to the male X chromosome. When bound to the male X, it also colocalizes with the dosage compensation protein male-specific lethal 3, suggesting a relationship to dosage compensation. The chromosome specificity is determined at the species level and not by the amino acid sequence. We argue that POF is involved in a chromosome-specific regulatory function.

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Section: Discussionmentioning

confidence: 89%

Section: Male X-specific Binding Is Conserved In Evolution Our Earlimentioning

confidence: 87%

Section: Male X-specific Binding Is Conserved In Evolution Our Earlimentioning

confidence: 94%

See 1 more Smart Citation

Painting of fourth in genus Drosophila suggests autosome-specific gene regulation

Larsson

Svensson

Stenberg

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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“…In a few described species, the F element is fused to one of the major chromosome arm. In Drosophila willistoni , the F element is fused to element E (Papaceit and Juan 1998), and in Drosophila busckii and Scaptodrosophila lebanonensis , the F element is fused to element A and thus is part of the X chromosome in these two species (Krivshenko 1955; Papaceit and Juan 1998). This may actually represent the ancestral state, i.e., the F element may originally have been part of the X chromosome (Fig.…”

Section: Autosome-specific Gene Regulationmentioning

confidence: 99%

Buffering and the evolution of chromosome-wide gene regulation

Stenberg

Larsson

2011

Chromosoma

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Copy number variation (CNV) in terms of aneuploidies of both entire chromosomes and chromosomal segments is an important evolutionary driving force, but it is inevitably accompanied by potentially problematic variations in gene doses and genomic instability. Thus, a delicate balance must be maintained between mechanisms that compensate for variations in gene doses (and thus allow such genomic variability) and selection against destabilizing CNVs. In Drosophila, three known compensatory mechanisms have evolved: a general segmental aneuploidy-buffering system and two chromosome-specific systems. The two chromosome-specific systems are the male-specific lethal complex, which is important for dosage compensation of the male X chromosome, and Painting of fourth, which stimulates expression of the fourth chromosome. In this review, we discuss the origin and function of buffering and compensation using Drosophila as a model.

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“…A dot chromosome is absent in D. willistoni , and chromosome III was hypothesized by Sturtevant and Novitski (1941) to have originated from the fusion of Muller elements E and F. Years later, Papaceit and Juan (1998) mapped three genes in D. willistoni by ISH, cubitus interrupitus ( ci ), ankyrin ( Ank ), and eyeless ( ey ), which are located in the D. melanogaster dot chromosome (Muller element F). They located the three genes in the centromeric region of D. willistoni chromosome III, corroborating with molecular techniques, the fusion of Muller elements E and F. This event has already been confirmed for all willistoni subgroup species ( Powell et al 2011 ; Pita et al 2014 ) with a timing of occurrence estimated at 15 Mya.…”

Section: Resultsmentioning

confidence: 99%

Reassignment of Drosophila willistoni Genome Scaffolds to Chromosome II Arms

Garcia

Delprat

Ruíz

et al. 2015

G3 Genes|Genomes|Genetics

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Drosophila willistoni is a geographically widespread Neotropical species. The genome of strain Gd-H4-1 from Guadeloupe Island (Caribbean) was sequenced in 2007 as part of the 12 Drosophila Genomes Project. The assembled scaffolds were joined based on conserved linkage and assigned to polytene chromosomes based on a handful of genetic and physical markers. This paucity of markers was particularly striking in the metacentric chromosome II, comprised two similarly sized arms, IIL and IIR, traditionally considered homologous to Muller elements C and B, respectively. In this paper we present the cytological mapping of 22 new gene markers to increase the number of markers mapped by in situ hybridization and to test the assignment of scaffolds to the polytene chromosome II arms. For this purpose, we generated, by polymerase chain reaction amplification, one or two gene probes from each scaffold assigned to the chromosome II arms and mapped these probes to the Gd-H4-1 strain’s polytene chromosomes by nonfluorescent in situ hybridization. Our findings show that chromosome arms IIL and IIR correspond to Muller elements B and C, respectively, directly contrasting the current homology assignments in D. willistoni and constituting a major reassignment of the scaffolds to chromosome II arms.

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Cited by 21 publications

References 18 publications

Painting of fourth in genus Drosophila suggests autosome-specific gene regulation

Painting of fourth in genus Drosophila suggests autosome-specific gene regulation

Buffering and the evolution of chromosome-wide gene regulation

Reassignment of Drosophila willistoni Genome Scaffolds to Chromosome II Arms

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