0033 Sleep Disruption Does Not Modify Sodium Intake Among Rats Fed A Cafeteria-style Diet

Abstract

Introduction: Sleep disruption (SD) and high sodium diets increase hypertension. We determined if SD increased sodium intake, preference for high sodium foods and if sex modified this effect when rats were fed a cafeteria-style diet (CAF-D). We hypothesized that SD would increase sodium intake and preference for savory foods independent of sex.Methods: Sprague-Dawley rats (n=8 male, n=20 female) were fed a CAF-D (rotating selection of 24 sweet and savory human foods + rodent chow ad libitum) and randomized to sleep undisturbed or SD due to environmental noise (8h/d, 16d). Calorie intake, weight gain and estrous cycle phase were determined daily.Results: In contrast to before treatment, undisturbed females gained significantly more weight than undisturbed males. Sleep disrupted males and females had similar weight gain. SD significantly increased calorie intake in males only. Independent of treatment or sex, rats preferred sweet relative to savory foods. Treatment didn't affect sodium intake but sleep disrupted males consumed significantly more sodium than females and ate more calories from savory foods than undisturbed males.Conclusions: Females are more sensitive to CAF-D than males. Despite that SD increased savory food intake in males, SD did not increase sodium consumption because these rats preferred sweet foods.