0.01% Hypochlorous Acid as an Alternative Skin Antiseptic: An In Vitro Comparison

Anagnostopoulos, Apostolos G.; Rong, Andrew J.; Miller, Darlene; Tran, Ann Q; Head, Trajen; Lee, Michael C.; Lee, Wendy W.

doi:10.1097/dss.0000000000001594

Cited by 21 publications

(9 citation statements)

References 16 publications

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“…This includes both HOCl externally added to wound beds and electrochemically produced HOCl (Wang et al 2007;Kiamco et al 2019). Treatment times for externally added HOCl are similar for bacterial and fungal biofilms (Anagnostopoulos et al 2018). As C. albicans can have differences between strains, further studies involving multiple such strains are needed to test our HOCl producing e-scaffold.…”

Section: Discussionmentioning

confidence: 99%

Hypochlorous acid-generating electrochemical scaffold eliminates Candida albicans biofilms

Zmuda

Mohamed

Raval

et al. 2020

Journal of Applied Microbiology

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Aims Wound infections involving Candida albicans can be challenging to treat because of the fungus’ ability to penetrate wound tissue and form biofilms. The goal of this study was to assess the activity of a hypochlorous acid (HOCl)‐generating electrochemical scaffold (e‐scaffold) against C. albicans biofilms in vitro and on porcine dermal explants (ex vivo). Methods and Results C. albicans biofilms were grown either on acrylic‐bottom six‐well plates (in vitro) or on skin tissue excised from porcine ears (ex vivo), and the polarized e‐scaffold was used to generate a continuous supply of low concentration HOCl near biofilm surfaces. C. albicans biofilms grown in vitro were reduced to undetectable amounts within 24 h of e‐scaffold exposure, unlike control biofilms (5·28 ± 0·034 log10 (CFU cm‐2); P < 0·0001). C. albicans biofilms grown on porcine dermal explants were also reduced to undetectable amounts in 24 h, unlike control explant biofilms (4·29 ± 0·057 log10 (CFU cm‐2); P < 0·0001). There was a decrease in the number of viable mammalian cells (35·6 ± 6·4%) in uninfected porcine dermal explants exposed to continuous HOCl‐generating e‐scaffolds for 24 h compared to explants exposed to nonpolarized e‐scaffolds (not generating HOCl) (P < 0·05). Conclusions Our HOCl‐generating e‐scaffold is a potential antifungal‐free strategy to treat C. albicans biofilms in chronic wounds. Significance and Impact of the Study Wound infections caused by C. albicans are difficult to treat due to presence of biofilms in wound beds. Our HOCl producing e‐scaffold provides a promising novel approach to treat wound infections caused by C. albicans.

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Section: Discussionmentioning

confidence: 99%

Hypochlorous acid-generating electrochemical scaffold eliminates Candida albicans biofilms

Zmuda

Mohamed

Raval

et al. 2020

Journal of Applied Microbiology

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“…Combinations of sodium hypochlorite (NaOCl) and hypochlorous acid (HOCl) are new formulations of antiseptic agents used for centuries. Antimicrobial activity relies on increasing the permeability of cells' walls, causing water to inflow into the cells that results in cell lysis [24]. The efficacy of hypochlorites is a controversial subject.…”

Section: Introductionmentioning

confidence: 99%

The High Impact of Staphylococcus aureus Biofilm Culture Medium on In Vitro Outcomes of Antimicrobial Activity of Wound Antiseptics and Antibiotic

et al. 2021

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The staphylococcal biofilm-based infections of wounds still pose a significant therapeutical challenge. Treated improperly, they increase the risk of limb amputation or even death of the patient. The present algorithms of infected wound treatment include, among others, the application of antiseptic substances. In vitro wound biofilm models are applied in order to scrutinize their activity. In the present work, using a spectrum of techniques, we showed how the change of a single variable (medium composition) in the standard in vitro model translates not only to shift in staphylococcal biofilm features but also to the change of efficacy of clinically applied wound antimicrobials such as octenidine, polyhexamethylene biguanide, chlorhexidine, hypochlorite solutions, and locally applied gentamycin. The data presented in this study may be of a pivotal nature, taking into consideration the fact that results of in vitro analyses are frequently used to propagate application of specific antimicrobials in hospitals and ambulatory care units.

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“…HOCl 0.01% had an immediate microbicidal effect on all organisms evaluated compared with commonly used highly concentrated skin antiseptics (isopropyl alcohol 70%, povidone iodine 5%, chlorhexidine gluconate 4%). 5 , 14 This feature allows its use even in particularly sensitive and inflamed areas. In addition, the HOCl ophthalmic spray formulation was easy and comfortable to handle, thus promoting continuity of treatment as suggested by the presented clinical cases and previous literature evidence.…”

Section: Discussionmentioning

confidence: 99%

The role of hypochlorous acid in the management of eye infections: a case series

Bertone¹,

Mollicone²,

Russo³

et al. 2022

DIC

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Experiences from clinical practice suggest that hypochlorous acid (HOCl) used as adjuvant treatment can shorten the time to clinical resolution of blepharitis, particularly in relation to the ophthalmic complications that often occur with a blepharitis diagnosis. In addition, in clinical practice, HOCl adjuvant treatment also resulted in a viable option for the management of ophthalmic disorders other than blepharitis. This case series presents and discusses real-life experiences with using an HOCl ophthalmic spray to manage eye infections, further characterizing the use of HOCl for ophthalmic applications.

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0.01% Hypochlorous Acid as an Alternative Skin Antiseptic: An In Vitro Comparison

Abstract: In vitro studies of HA 0.01% were observed to have equal or more efficacious antiseptic properties compared with IPA, CHG, and PI. Future studies will be needed to investigate its role in periocular use.

Cited by 21 publications

References 16 publications

Hypochlorous acid-generating electrochemical scaffold eliminates Candida albicans biofilms

Hypochlorous acid-generating electrochemical scaffold eliminates Candida albicans biofilms

The High Impact of Staphylococcus aureus Biofilm Culture Medium on In Vitro Outcomes of Antimicrobial Activity of Wound Antiseptics and Antibiotic

The role of hypochlorous acid in the management of eye infections: a case series

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