Experiments were performed to study electron acceleration by intense sub-picosecond laser pulses propagating in sub-mm long plasmas of near critical electron density (NCD). Low density foam layers of 300-500 μm thickness were used as targets. In foams, the NCD-plasma was produced by a mechanism of super-sonic ionization when a well-defined separate ns-pulse was sent onto the foamtarget forerunning the relativistic main pulse. The application of sub-mm thick low density foam layers provided a substantial increase of the electron acceleration path in a NCD-plasma compared to the case of freely expanding plasmas created in the interaction of the ns-laser pulse with solid foils. The performed experiments on the electron heating by a 100 J, 750 fs short laser pulse of 2-5×10 19 W cm −2 intensity demonstrated that the effective temperature of supra-thermal electrons increased from 1.5-2 MeV in the case of the relativistic laser interaction with a metallic foil at high laser contrast up to 13 MeV for the laser shots onto the pre-ionized foam. The observed tendency towards a strong increase of the mean electron energy and the number of ultra-relativistic laseraccelerated electrons is reinforced by the results of gamma-yield measurements that showed a 1000fold increase of the measured doses. The experiment was supported by 3D-PIC and FLUKA simulations, which considered the laser parameters and the geometry of the experimental set-up. Both, measurements and simulations showed a high directionality of the acceleration process, since the strongest increase in the electron energy, charge and corresponding gamma-yield was observed close to the direction of the laser pulse propagation. The charge of super-ponderomotive electrons with energy above 30 MeV reached a very high value of 78 nC.
Bioluminescent solid‐phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer–target complexes during design and development of the specific aptamers. The assay involves Ca2+‐regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.
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