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A B S T R A C T Viral noncoding (nc) RNAs have been shown to play important roles in viral life cycle. Many viruses employ different mechanism to produce ncRNAs. Here, we report that coronavirus infectious bronchitis virus (IBV) produces a novel ncRNA in virus-infected cells. This ncRNA consists of 563 nucleotides excluding a poly(A) tail, is mainly derived from the 3′-untranslated region of IBV genome, and contains a 63-nt-long of terminal leader sequence derived from the 5′ end of the viral genome. Using mutagenesis and reverse genetics, we reveal that this ncRNA is a subgenomic RNA generated by discontinuous transcription mechanism.

Leakage of stray current can cause serious problems by accelerating the corrosion process of a buried pipeline in the subway. A multi-physical finite element model of the DC-subway traction system was established in this study, and the dynamic process of the stray current corrosion on buried pipeline was calculated according to the real time traction conditions. In this study, the corrosion rate variation of stray current is evaluated, and the corrosion trend of stray current is quantitatively calculated. The model simulation shows that the stray current corrosion is significantly higher than other processes during the acceleration process of the subway locomotive. And the potential of the buried pipeline reaches a maximum value when an up-rush of traction current occurs. The corrosion is mainly concentrated in the anode region of the buried pipeline, and the closer the buried pipeline is to the rail the more serious the corrosion is. A corrosion experiment of N20 carbon steel was carried out and verified by finite element model. The results further show that the finite element model can quantitatively calculate and predict the mass loss of buried metal caused by stray current corrosion.