The clinical picture of severe acute respiratory syndrome (SARS) is characterized by an over-exuberant immune response with lung lymphomononuclear cells infilteration and proliferation that may account for tissue damage more than the direct effect of viral replication. To understand how cells response in the early stage of virus-host cell interaction, in this study, a purified recombinant S protein was studied for stimulating murine macrophages (RAW264.7) to produce proinflammatory cytokines (IL-6 and TNF-alpha) and chemokine IL-8. We found that direct induction of IL-6 and TNF-alpha release in the supernatant in a dose-, time-dependent manner and highly spike protein-specific, but no induction of IL-8 was detected. Further experiments showed that IL-6 and TNF-alpha production were dependent on NF-kappaB, which was activated through I-kappaBalpha degradation. These results suggest that SARS-CoV spike protein may play an important role in the pathogenesis of SARS, especially in inflammation and high fever.

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The fundamental mechanism initiating coronal mass ejections (CMEs) remains controversial. One of the leading theories is magnetic breakout, in which magnetic reconnection occurring high in the corona removes the confinement on an energized low-corona structure from the overlying magnetic field, thus allowing it to erupt. Here, we report critical observational evidence of this elusive breakout reconnection in a multi-polar magnetic configuration that leads to a CME and an X-class, long-duration flare. Its occurrence is supported by the presence of pairs of heated cusp-shaped loops around an X-type null point and signatures of reconnection inflows. Other peculiar features new to the breakout picture include sequential loop brightening, coronal hard X-rays at energies up to ∼100 keV, and extended high-corona X-rays above the later restored multi-polar structure. These observations, from a novel perspective with clarity never achieved before, present crucial clues to understanding the initiation mechanism of solar eruptions.

Between July 5th and July 7th 2004, two intriguing fast coronal mass ejection(CME)-streamer interaction events were recorded by the Large Angle and Spectrometric Coronagraph (LASCO). At the beginning of the events, the streamer was pushed aside from their equilibrium position upon the impact of the rapidly outgoing and expanding ejecta; then, the streamer structure, mainly the bright streamer belt, exhibited elegant large scale sinusoidal wavelike motions. The motions were apparently driven by the restoring magnetic forces resulting from the CME impingement, suggestive of magnetohydrodynamic kink mode propagating outwards along the plasma sheet of the streamer. The mode is supported collectively by the streamer-plasma sheet structure and is therefore named " streamer wave" in the present study. With the white light coronagraph data, we show that the streamer wave has a period of about 1 hour, a wavelength varying from 2 to 4 solar radii, an amplitude of about a few tens of solar radii, and a propagating phase speed in the range 300 to 500 km s −1 . We also find that there is a tendancy for the phase speed to decline with increasing heliocentric distance. These observations provide good examples of large scale wave phenomena carried by coronal structures, and have significance in developing seismological techniques for diagnosing plasma and magnetic parameters in the outer corona.

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