Phenolic compounds were extracted from thyme (Thymus vulgaris L.), oregano (Origanum vulgare L.) and marjoram (Origanum majorana L.) with 95% ethanol. A number of antioxidant and radical-scavenging capacity tests were performed on the prepared extracts using colorimetric assays and model system studies. Specifically, these included determining the content of total phenolics, antioxidant efficacy in a linoleic acid-ferric thiocyanate model system, reducing power, scavenging effect on 2,2'-diphenyl-1-picrylhydrazyl radical, and hydroxyl radical-scavenging activity by electron paramagnetic resonance spectroscopy. Moreover, the efficacies of the prepared herb extracts were investigated in a real-life food product: the stabilization of butter against oxidation.
Amarowicz R., Żegarska Z., Pegg R. B., Karamać M., Kosińska A. (2007): Antioxidant and radical scavenging activities of a barley crude extract and its fraction. Czech J. Food Sci., 25: 73-80.Phenolic compounds were extracted from the Candle variety of hull-less waxy barley with 80% (v/v) methanol to yield a crude preparation. Seven fractions (I-VII) were separated from the barley extract so obtained on a Sephadex LH-20 column using methanol as the mobile phase. Nearly 80% of the phenolics extracted from barley were comprised in the first three fractions. The measurements of the antioxidant activity using a β-carotene-linoleate model system, radical scavenging capacity against DPPH•, and reducing power based on the reduction of a Fe 3+ /K 3 Fe(CN) 6 complex to the ferrous state were assessed in the barley crude extract and its fractions. The results indicated that barley possess marked antioxidant and antiradical capacities as compared to other grains such as wheat, rye, and triticale. Furthermore, the methanolic extract of the waxy barley sample and its fractions resembled in the aforementioned activities those from leguminous seeds, rapeseed and pulses. Phenolic constituents contained in barley may have a future role as ingredients in the development of functional foods.
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