No abstract

A Gram-negative, short rod-shaped bacterium, strain GA2-M15 T , was isolated from a sea-sand sample at Homi Cape, Pohang city, Republic of Korea. 16S rRNA gene sequence analysis demonstrated that this isolate was unique, showing 95.9 % sequence similarity to the type strain of Thalassobacter stenotrophicus and similarities of 94.0-95.2 % to the type strains of species of the genera Octadecabacter (94.4-95.2 %), Jannaschia (94.0-94.4 %) and Thalassobius (94.0-94.7 %). Chemotaxonomic characteristics (diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine as the major polar lipids and C 18 : 1 v7c as the predominant fatty acid) and DNA G+C content (56 mol%) were also similar to those of Thalassobacter stenotrophicus. 16S rRNA gene sequence similarity, physiological properties and some fatty acid components showed that strain GA2-M15T could be differentiated fromThalassobacter stenotrophicus. On the basis of these results, strain GA2-M15 T is considered to represent a novel species of the genus Thalassobacter, for which the name Thalassobacter arenae sp. nov. is proposed. The type strain is GA2-M15 T (5KACC 12675The genus Thalassobacter was described to accommodate a single bacterial strain isolated from Mediterranean seawater (Macián et al., 2005). A subsequent polyphasic taxonomic approach revealed that the type strains of Jannaschia cystaugens (Adachi et al., 2004) and Thalassobacter stenotrophicus (Macián et al., 2005) were highly similar and should be considered to be members of the same species. Pujalte et al. (2005) thus proposed the unification of the two species as Thalassobacter stenotrophicus and provided an emended description of the genus Thalassobacter. Phylogenetically, this genus is a member of the Alphaproteobacteria, and is closely related to the genera Jannaschia, Octadecabacter and Thalassobius. Thalassobacter was characterized as comprising Gramnegative, strictly aerobic, chemo-organotrophic, slightly halophilic bacteria, with C 18 : 1 v7c as the major fatty acid and phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine as the major polar lipids.Strain GA2-M15 T was isolated from a sea-sand sample collected from the coast of Homi Cape, Pohang city, Republic of Korea. Isolation of the strain was performed by using the standard dilution-plating method on marine agar 2216 (MA; Difco) kept at 30 u C for 10 days. The culture was maintained routinely on MA at 30 uC and was preserved as glycerol suspensions (15 %, v/v) at 280 u C. Strain GA2-M15 T grew weakly on nutrient agar (NA), but did not grow on R2A, trypticase soy agar (TSA) or MacConkey agar (all from Difco). Thalassobacter stenotrophicus DSM 16310 T was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig, Germany, and was used as a reference strain.The methods described by Smibert & Krieg (1994) were used for Gram staining and to detect accumulation of polyhydroxybutyrate, the presence of catalase and oxidase, and hydrolysis of casein, DNA and sta...

Abstract:The temperate and herbaceous genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae. The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, but also extending to the temperate regions of South America and tropical Africa. The use of simple sequence repeat (SSR) markers for Vicia species has not been investigated as extensively as for other crop species. In this study, we assessed the potential for cross-species amplification of cDNA microsatellite markers developed from common vetch (Vicia sativa subsp. sativa). For cross-species amplification of the SSRs, amplification was carried out with genomic DNA isolated from two to eight accessions of 22 different Vicia species. For individual species or subspecies, the transferability rates ranged from 33% for V. ervilia to 82% for V. sativa subsp. nigra with an average rate of 52.0%. Because the rate of successful SSR marker amplification generally correlates with genetic distance, these SSR markers are potentially useful for analyzing genetic relationships between or within Vicia species.

Two aerobic, Gram-positive, rod-shaped bacterial strains, 5YN10-14T and GR21-5T, were isolated from the Yongneup wetland and ginseng soil in Korea, respectively. The two strains formed ellipsoidal or oval spores positioned centrally or paracentrally in swollen sporangia. On the basis of 16S rRNA gene sequence analysis, these strains were related to members of the genus Cohnella. 16S rRNA gene sequence similarity between strains 5YN10-14T and GR21-5T was 95.9 %. Strains 5YN10-14T and GR21-5T showed, respectively, 94.3 and 95.2 % 16S rRNA gene sequence similarity to Cohnella thermotolerans CCUG 47242T, 94.6 and 94.4 % to Cohnella hongkongensis HKU3T, 94.7 and 94.7 % to Cohnella laeviribosi RI-39T, and 95.4 and 94.8 % to Cohnella phaseoli GSPC1T. The major fatty acids of strain 5YN10-14T were anteiso-C15 : 0 (51.1 %), iso-C16 : 0 (18.5 %) and C16 : 0 (13.2 %), and the major fatty acids of strain GR21-5T were anteiso-C15 : 0 (48.9 %), iso-C16 : 0 (15.0 %) and iso-C15 : 0 (12.2 %). The two strains contained menaquinone with seven isoprene units (MK-7) as the predominant quinone, and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as major polar lipids; however, strain 5YN10-14T also contained lysylphosphatidylglycerol as a major polar lipid, whereas strain GR21-5T had an unknown aminophospholipid as another major polar lipid. The DNA G+C contents of strains 5YN10-14T and GR21-5T were 58.8 and 61.3 mol%, respectively. Based on the results of the phylogenetic and phenotypic data presented, it was concluded that the two strains represent two novel species of the genus Cohnella, for which the names Cohnella yongneupensis sp. nov. (type strain 5YN10-14T=KACC 11768T=DSM 18998T) and Cohnella ginsengisoli sp. nov. (type strain GR21-5T=KACC 11771T=DSM 18997T) are proposed.