The study investigated the influence of sewage sludge application at rates of 0 (CK), 30 (ST), 75 (MT), and 150 (HT) t ha−1 to mudflats on bacterial community diversity and predicted functions using amplicon-based sequencing. Soils under sewage sludge treatments, especially the HT treatment, exhibited lower pH, salinity and higher nutrient contents (C, N, and P). Moreover, restructured bacterial communities with significantly higher diversities and distinct core and unique microbiomes were observed in all sewage sludge-amended soils as compared to the control. Specifically, core bacterial families, such as Hyphomicrobiaceae, Cytophagaceae, Pirellulaceae Microbacteriaceae, and Phyllobacteriaceae, were significantly enriched in sewage sludge-amended soils. In addition, sewage sludge amendment significantly improved predicted functional diversities of core microbiomes, with significantly higher accumulative relative abundances of functions related to carbon and nitrogen cycling processes compared to the unamended treatment. Correlation analyses showed that modified soil physicochemical properties were conducive for the improvement of diversities of bacterial communities and predicted functionalities. These outcomes demonstrated that sewage sludge amendment not only alleviated saline–sodic and nutrient deficiency conditions, but also restructured bacterial communities with higher diversities and versatile functions, which may be particularly important for the fertility formation and development of mudflat soils.
China is facing a shortage of arable land resources, and the mudflat salt-affected soil along the east coast of China is an important reserve arable land resource. In this study, we conducted a randomized field trial to investigate the effects of vermicompost application rate (0, 25, 50, 125, and 250 t ha−1) on barley growth and heavy metal accumulation in mudflat salt-affected soil. We found that vermicompost application decreased bulk density, electrical conductivity (EC), and pH of mudflat salt-affected soil while increasing its organic carbon, nitrogen, and phosphorus contents. With the increase in vermicompost application rate, the yield of grain and total biomass of barley plants increased. The yield of grain in the vermicompost application treatments of 25, 50, 125, and 250 t ha−1 increased by 66.0%, 226.0%, 340.0%, and 512.0%, respectively, relative to the control. In addition, the concentrations of heavy metals (Cd, Cr, Cu, and Zn) in mudflat salt-affected soil and barley plant increased as the vermicompost application rate increased.
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