This study investigated the differences in microbial community abundance, composition, and diversity throughout the depth profiles in soils collected from corn and soybean fields in Iowa (United States) using 16S rRNA amplicon sequencing. The results revealed decreased richness and diversity in microbial communities at increasing soil depth. Soil microbial community composition differed due to crop type only in the top 60 cm and due to location only in the top 90 cm. While the relative abundance of most phyla decreased in deep soils, the relative abundance of the phylum Proteobacteria increased and dominated agricultural soils below the depth of 90 cm. Although soil depth was the most important factor shaping microbial communities, edaphic factors, including soil organic matter, soil bulk density, and the length of time that deep soils were saturated with water, were all significant factors explaining the variation in soil microbial community composition. Soil organic matter showed the highest correlation with the exponential decrease in bacterial abundance with depth. A greater understanding of how soil depth influences the diversity and composition of soil microbial communities is vital for guiding sampling approaches in agricultural soils where plant roots extend beyond the upper soil profile. In the long term, a greater knowledge of the influence of depth on microbial communities should contribute to new strategies that enhance the sustainability of soil, which is a precious resource for food security. IMPORTANCE Determining how microbial properties change across different soils and within the soil depth profile will be potentially beneficial to understanding the long-term processes that are involved in the health of agricultural ecosystems. Most literature on soil microbes has been restricted to the easily accessible surface soils. However, deep soils are important in soil formation, carbon sequestration, and providing nutrients and water for plants. In the most productive agricultural systems in the United States where soybean and corn are grown, crop plant roots extend into the deeper regions of soils (>100 cm), but little is known about the taxonomic diversity or the factors that shape deep-soil microbial communities. The findings reported here highlight the importance of soil depth in shaping microbial communities, provide new information about edaphic factors that influence the deep-soil communities, and reveal more detailed information on taxa that exist in deep agricultural soils.
Revealing the unexplored rhizosphere microbiome of plants in arid environments can help in understanding their interactions between microbial communities and plants during harsh growth conditions. Here, we report the first investigation of rhizospheric fungal and bacterial communities of Adenium obesum, Aloe dhufarensis and Cleome austroarabica using next-generation sequencing approaches. A. obesum and A. dhufarensis grows in dry tropical and C. austroarabica in arid conditions of Arabian Peninsula. The results indicated the presence of 121 fungal and 3662 bacterial operational taxonomic units (OTUs) whilst microbial diversity was significantly high in the rhizosphere of A. obesum and A. dhufarensis and low in C. austroarabica. Among fungal phyla, Ascomycota and Basidiomycota were abundantly associated within rhizospheres of all three plants. However, Mucoromycota was only present in the rhizospheres of A. obesum and A. dhufarensis, suggesting a variation in fungal niche on the basis of host and soil types. In case of bacterial communities, Actinobacteria, Proteobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, and Verrucomicrobia were predominant microbial phyla. These results demonstrated varying abundances of microbial structure across different hosts and locations in arid environments. Rhizosphere’s extracellular enzymes analysis revealed varying quantities, where, glucosidase, cellulase, esterase, and 1-aminocyclopropane-1-carboxylate deaminase were significantly higher in the rhizosphere of A. dhufarensis, while phosphatase and indole-acetic acid were highest in the rhizosphere of A. obesum. In conclusion, current findings usher for the first time the core microbial communities in the rhizospheric regions of three arid plants that vary greatly with location, host and soil conditions, and suggest the presence of extracellular enzymes could help in maintaining plant growth during the harsh environmental conditions.
Much effort has been placed on developing microbial inoculants to replace or supplement fertilizers to improve crop productivity and environmental sustainability. However, many studies ignore the dynamics of plant-microbe interactions and the genotypic specificity of the host plant on the outcome of microbial inoculation. Thus, it is important to study temporal plant responses to inoculation in multiple genotypes within a single species. With the implementation of high-throughput phenotyping, the dynamics of biomass and nitrogen (N) accumulation of four sorghum genotypes with contrasting N-use efficiency were monitored upon the inoculation with synthetic microbial communities (SynComs) under high and low-N. Five SynComs comprising bacteria isolated from field grown sorghum were designed based on the overall phylar composition of bacteria and the enriched host compartment determined from a field-based culture independent study of the sorghum microbiome. We demonstrated that the growth response of sorghum to SynCom inoculation is genotype-specific and dependent on plant N status. The sorghum genotypes that were N-use inefficient were more susceptible to the colonization from a diverse set of inoculated bacteria as compared to the N-use efficient lines especially under low-N. By integrating high-throughput phenotyping with sequencing data, our findings highlight the roles of host genotype and plant nutritional status in determining colonization by bacterial synthetic communities.
17 The determination of how microbial community structure changes within the soil profile, 18 will be beneficial to understanding the long-term health of agricultural soil ecosystems 19 and will provide a first step towards elucidating how deep soil microbial communities 20 contribute to carbon sequestration. This study aimed to investigate the differences in the 21 microbial community abundance, composition and diversity throughout from the surface 22 layers down to deep soils in corn and soybean fields in Iowa, USA. We used 16S rRNA 23 amplicon sequencing of soil samples to characterize the change in microbial community 24 structure. Our results revealed decreased richness and diversity in bacterial community 25 structure with increasing soil depth. We also observed distinct distribution patterns of 26 bacterial community composition along soil profiles. Soil and root data at different 27 depths enabled us to demonstrate that the soil organic matter, soil bulk density and 28 plant water availability were all significant factors in explaining the variation in soil 29 microbial community composition. Our findings provide valuable insights in the changes 30 in microbial community structure to depths of 180 cm in one of the most productive 31 agricultural regions in the world. This knowledge will be important for future 32 management and productivity of agroecosystems in the face of increasing demand for 33 food and climate change. 34 35 36
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